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The map label for this gene is cysH [H]

Identifier: 183220580

GI number: 183220580

Start: 1232476

End: 1233201

Strand: Reverse

Name: cysH [H]

Synonym: LEPBI_I1184

Alternate gene names: 183220580

Gene position: 1233201-1232476 (Counterclockwise)

Preceding gene: 183220581

Following gene: 183220579

Centisome position: 34.26

GC content: 44.63

Gene sequence:

>726_bases
ATGGGAAATTTGGAAGTTTTGACAGAATCTTATGCCAACCGCTCGCTTGTGGATGGTTTACGCCAATTGAGCTCCGAATT
CCCGGGCAAAGTGGTCTTCACCACAAGTTTCGGACTCGAAGACCAAGCCATCACCCATGCCATCCTATCCGAACAAATCC
CCATCCGCATCGCCACTTTGGATACTGGACGGCTTTTCCAAGAAACCTATGATGTTTGGCAAAAAACAAACATTCGTTAT
GGGGCAAATATTGAAACCCATTTTCCCAATACAAAAGAGATCCAAAATTTTGTGGATACAAACGGCCCCAATGCATTTTA
CGATTCGCAAGACCTAAGAAAAGAATGTTGTCGGATCCGAAAACTAGTCCCTCTTGATTCCTTACTCACTGGGATGGAAG
TTTGGGTGACTGGCCTTAGAAAAGACCAATCCGGATTTCGGACAGAGATGTCTTTATTCGAATCCGATCCAGCACGAAAT
TTAATCAAATACCAACCCCTACTCCATTGGAGTTTTGAAGATACTTGGAAGTACATCCGCGAACACAATGTACCTTATAA
TCTTTTGCATGACAAAGGATTCCCAAGCATCGGTTGTGCACCATGCACACGAGCCATCGAACCGGGCGAAGACTTTCGTG
CGGGAAGGTGGTGGTGGGAACAAGAATCTAAGAAAGAGTGTGGTTTGCATTGGGTAGATGGAAAACTCACACCGAAAAAA
GGATAA

Upstream 100 bases:

>100_bases
CAAAAGTTTACGTAGAAGACTTCTCTATCTAATAATTTCCGCTAATATACTTTACAAATCGTCCAATATACAAAACATTG
GATGCTAGGACGGGGAAAAT

Downstream 100 bases:

>100_bases
CAGGCCATGACAAACACACATCGACTTTCACATTTAGACCAATTAGAATCAGAAGCAATTTATATCTTACGGGAAGTAGC
TGCCCAATTCGAAAGACCCG

Product: 5'-adenylylsulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MGNLEVLTESYANRSLVDGLRQLSSEFPGKVVFTTSFGLEDQAITHAILSEQIPIRIATLDTGRLFQETYDVWQKTNIRY
GANIETHFPNTKEIQNFVDTNGPNAFYDSQDLRKECCRIRKLVPLDSLLTGMEVWVTGLRKDQSGFRTEMSLFESDPARN
LIKYQPLLHWSFEDTWKYIREHNVPYNLLHDKGFPSIGCAPCTRAIEPGEDFRAGRWWWEQESKKECGLHWVDGKLTPKK
G

Sequences:

>Translated_241_residues
MGNLEVLTESYANRSLVDGLRQLSSEFPGKVVFTTSFGLEDQAITHAILSEQIPIRIATLDTGRLFQETYDVWQKTNIRY
GANIETHFPNTKEIQNFVDTNGPNAFYDSQDLRKECCRIRKLVPLDSLLTGMEVWVTGLRKDQSGFRTEMSLFESDPARN
LIKYQPLLHWSFEDTWKYIREHNVPYNLLHDKGFPSIGCAPCTRAIEPGEDFRAGRWWWEQESKKECGLHWVDGKLTPKK
G
>Mature_240_residues
GNLEVLTESYANRSLVDGLRQLSSEFPGKVVFTTSFGLEDQAITHAILSEQIPIRIATLDTGRLFQETYDVWQKTNIRYG
ANIETHFPNTKEIQNFVDTNGPNAFYDSQDLRKECCRIRKLVPLDSLLTGMEVWVTGLRKDQSGFRTEMSLFESDPARNL
IKYQPLLHWSFEDTWKYIREHNVPYNLLHDKGFPSIGCAPCTRAIEPGEDFRAGRWWWEQESKKECGLHWVDGKLTPKKG

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=206, Percent_Identity=26.2135922330097, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325425, Length=94, Percent_Identity=44.6808510638298, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 27835; Mature: 27704

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNLEVLTESYANRSLVDGLRQLSSEFPGKVVFTTSFGLEDQAITHAILSEQIPIRIATL
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEE
DTGRLFQETYDVWQKTNIRYGANIETHFPNTKEIQNFVDTNGPNAFYDSQDLRKECCRIR
CCHHHHHHHHHHHHHCCCEECCEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KLVPLDSLLTGMEVWVTGLRKDQSGFRTEMSLFESDPARNLIKYQPLLHWSFEDTWKYIR
HCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCEEECCHHHHHHHHH
EHNVPYNLLHDKGFPSIGCAPCTRAIEPGEDFRAGRWWWEQESKKECGLHWVDGKLTPKK
HCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCEEECCEECCCC
G
C
>Mature Secondary Structure 
GNLEVLTESYANRSLVDGLRQLSSEFPGKVVFTTSFGLEDQAITHAILSEQIPIRIATL
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEE
DTGRLFQETYDVWQKTNIRYGANIETHFPNTKEIQNFVDTNGPNAFYDSQDLRKECCRIR
CCHHHHHHHHHHHHHCCCEECCEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KLVPLDSLLTGMEVWVTGLRKDQSGFRTEMSLFESDPARNLIKYQPLLHWSFEDTWKYIR
HCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCEEECCHHHHHHHHH
EHNVPYNLLHDKGFPSIGCAPCTRAIEPGEDFRAGRWWWEQESKKECGLHWVDGKLTPKK
HCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCEEECCEECCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]