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The map label for this gene is dfpA [H]

Identifier: 183220130

GI number: 183220130

Start: 733563

End: 734093

Strand: Direct

Name: dfpA [H]

Synonym: LEPBI_I0718

Alternate gene names: 183220130

Gene position: 733563-734093 (Clockwise)

Preceding gene: 183220129

Following gene: 183220131

Centisome position: 20.38

GC content: 44.26

Gene sequence:

>531_bases
ATGAAAGAAATCATCATCGCAGTCTCAGGTTCCATTGCTTCTTACAAGGCATGTGATTTGGTAAGAGGGCTTACCAAAAC
TGGTTACCCTGTGCGAGTGATCATGACTTCCAACGCCACAAAATTTGTGGGTAAAATTACCTTCGAAGCCCTAACAGGAA
AACCTGTACGTGTGGATGAATTTGATACAGGGATGGCCCATATCGAAATCAAAAACATTGCGTCTGTGTTTGCCGTCGTT
CCTGCTTCCGCCAATATCATTGGAAAAATGGCAAACGGAATTGCCGATGATTTAGTGACGTCAACCTATCTCGCTTGCAC
CTCACCTGTATTAGTTGCTCCTTCGATGAATCCTGGGATGTATCTCCATCCAGCAGTGCAGAGGAATTTAAAAACACTAG
AGGCCGATGGCGTACACATTGTATCCCCAGAAAAGGGCATAGTGGTTTGTGGTGATGAAGGGTATGGCAAACTGGCGACA
GTCGAAACCATTATGGAACAAATCATCGAACTCCACAAAAAGAATTCATGA

Upstream 100 bases:

>100_bases
AATGGAAGGAAACAAAATCATTTCCATCCTTTTCACGCCGAAATTAGAAGAAGACCATGATTCCGAAATGGAACGGGAAC
TCTCTTACGAGGATCGGTAA

Downstream 100 bases:

>100_bases
ATTTAAAATTCAAACGAGTCATTGTCACCTCAGGACCCACGAGAGAATGGATTGACCCAGTTCGGTACATCTCGAATGCC
TCATCTGGCAAAATGGGATA

Product: phosphopantothenoylcysteine decarboxylase

Products: NA

Alternate protein names: DNA/pantothenate metabolism flavoprotein; Phosphopantothenoylcysteine decarboxylase; PPCDC; CoaC; Phosphopantothenate--cysteine ligase; CoaB; PPC synthetase; PPCS; Phosphopantothenoylcysteine synthase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MKEIIIAVSGSIASYKACDLVRGLTKTGYPVRVIMTSNATKFVGKITFEALTGKPVRVDEFDTGMAHIEIKNIASVFAVV
PASANIIGKMANGIADDLVTSTYLACTSPVLVAPSMNPGMYLHPAVQRNLKTLEADGVHIVSPEKGIVVCGDEGYGKLAT
VETIMEQIIELHKKNS

Sequences:

>Translated_176_residues
MKEIIIAVSGSIASYKACDLVRGLTKTGYPVRVIMTSNATKFVGKITFEALTGKPVRVDEFDTGMAHIEIKNIASVFAVV
PASANIIGKMANGIADDLVTSTYLACTSPVLVAPSMNPGMYLHPAVQRNLKTLEADGVHIVSPEKGIVVCGDEGYGKLAT
VETIMEQIIELHKKNS
>Mature_176_residues
MKEIIIAVSGSIASYKACDLVRGLTKTGYPVRVIMTSNATKFVGKITFEALTGKPVRVDEFDTGMAHIEIKNIASVFAVV
PASANIIGKMANGIADDLVTSTYLACTSPVLVAPSMNPGMYLHPAVQRNLKTLEADGVHIVSPEKGIVVCGDEGYGKLAT
VETIMEQIIELHKKNS

Specific function: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine [H]

COG id: COG0452

COG function: function code H; Phosphopantothenoylcysteine synthetase/decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PPC synthetase family [H]

Homologues:

Organism=Homo sapiens, GI71725351, Length=186, Percent_Identity=31.1827956989247, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI87082301, Length=172, Percent_Identity=38.953488372093, Blast_Score=122, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560194, Length=176, Percent_Identity=30.1136363636364, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322762, Length=195, Percent_Identity=30.2564102564103, Blast_Score=81, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24657262, Length=186, Percent_Identity=31.7204301075269, Blast_Score=83, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005252
- InterPro:   IPR007085
- InterPro:   IPR003382 [H]

Pfam domain/function: PF04127 DFP; PF02441 Flavoprotein [H]

EC number: =4.1.1.36; =6.3.2.5 [H]

Molecular weight: Translated: 18750; Mature: 18750

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIIIAVSGSIASYKACDLVRGLTKTGYPVRVIMTSNATKFVGKITFEALTGKPVRVDE
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEEECCCCCEEECC
FDTGMAHIEIKNIASVFAVVPASANIIGKMANGIADDLVTSTYLACTSPVLVAPSMNPGM
CCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCE
YLHPAVQRNLKTLEADGVHIVSPEKGIVVCGDEGYGKLATVETIMEQIIELHKKNS
EECHHHHHCHHEECCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEIIIAVSGSIASYKACDLVRGLTKTGYPVRVIMTSNATKFVGKITFEALTGKPVRVDE
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEEECCCCCEEECC
FDTGMAHIEIKNIASVFAVVPASANIIGKMANGIADDLVTSTYLACTSPVLVAPSMNPGM
CCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCE
YLHPAVQRNLKTLEADGVHIVSPEKGIVVCGDEGYGKLATVETIMEQIIELHKKNS
EECHHHHHCHHEECCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]