Click here to switch to the map view.

The map label for this gene is tktB [C]

Identifier: 183220057

GI number: 183220057

Start: 648747

End: 649559

Strand: Direct

Name: tktB [C]

Synonym: LEPBI_I0644

Alternate gene names: 183220057

Gene position: 648747-649559 (Clockwise)

Preceding gene: 183220056

Following gene: 183220058

Centisome position: 18.02

GC content: 39.85

Gene sequence:

>813_bases
ATGGAAAAAGTTGAAGTCGCAAAGAAATTTGCAAAAGATATCCGAATCCAAGTAATCAAAATGGTTACAGCTGCCAATTC
TGGCCACCCAGGTGGACCACTTGGACTTGCTGATATCTACGCTGCACTTTACACTTCCATTTTAAACCACGATCCTAAAA
ATCCAGAATTGCCAGAAAGAGATCGATTGATTTTATCCAACGGACACGTTTGCGCCGTTCGTTATGCCGCAATGGGACTT
TCTGGTTACTTCCCAGTGGAAGACCTTCTCACGTTTCGTAATATCAATTCCTACTTACAAGGCCACCCATCCACTCGTTA
TATGAAAGGGATTGAGTCTAGTTCTGGTTCCCTTGGACAAGGTTTATCTGTATCGGTTGGACTTGCCCTTGGTGCAAAAC
TAAAAAAAGAGACATATAAAATTTATACATGCATTTCTGATGGGGAATGTGGTGAAGGGATGACTTGGGAAGCAGCACAA
TCTGCTGTTCACTTCAAAACGGATAACCTCATTGCCTTTATGGATCGTAACTACATTCAAATTGATGGTAATACAGAAGA
AGTCATGAAGTTAGAACCACTTGATAAAAAATTTGAAATGTTTGGTTGGAATGTGATCAATGCAGACGGACATAATATGG
AAGAAATTTTTTCTGCATTTGCCAAAGCAAAACAACATACAGGTGGACCAACGCTGATTGTCTTTAGAACCATTTTAGGG
AAAGGTGTTTCTTATATGGAAAACAATCCGAAATGGCATGGCACTCCACCAAATAAAGAACAAGAAGCACAAGCTCTTGC
AGAATTAGCTTAA

Upstream 100 bases:

>100_bases
TCTACATGTAAAATTGCATTACATCTTTTTGCCATTGTACACGATTGTTTATATCTCTGGTCTGTTTTTATTTCAATCAA
AACCGCTTTAAGGAATGTTC

Downstream 100 bases:

>100_bases
AGTTTTTGTTTCGATTGACTTTTTCATTTTAAGTTAGATAGTTTTAGCATGGGACAAAACTCTTTTCCTGATTTTTACCC
GGATGATATCACTCGTTTAT

Product: putative transketolase

Products: NA

Alternate protein names: TK [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MEKVEVAKKFAKDIRIQVIKMVTAANSGHPGGPLGLADIYAALYTSILNHDPKNPELPERDRLILSNGHVCAVRYAAMGL
SGYFPVEDLLTFRNINSYLQGHPSTRYMKGIESSSGSLGQGLSVSVGLALGAKLKKETYKIYTCISDGECGEGMTWEAAQ
SAVHFKTDNLIAFMDRNYIQIDGNTEEVMKLEPLDKKFEMFGWNVINADGHNMEEIFSAFAKAKQHTGGPTLIVFRTILG
KGVSYMENNPKWHGTPPNKEQEAQALAELA

Sequences:

>Translated_270_residues
MEKVEVAKKFAKDIRIQVIKMVTAANSGHPGGPLGLADIYAALYTSILNHDPKNPELPERDRLILSNGHVCAVRYAAMGL
SGYFPVEDLLTFRNINSYLQGHPSTRYMKGIESSSGSLGQGLSVSVGLALGAKLKKETYKIYTCISDGECGEGMTWEAAQ
SAVHFKTDNLIAFMDRNYIQIDGNTEEVMKLEPLDKKFEMFGWNVINADGHNMEEIFSAFAKAKQHTGGPTLIVFRTILG
KGVSYMENNPKWHGTPPNKEQEAQALAELA
>Mature_270_residues
MEKVEVAKKFAKDIRIQVIKMVTAANSGHPGGPLGLADIYAALYTSILNHDPKNPELPERDRLILSNGHVCAVRYAAMGL
SGYFPVEDLLTFRNINSYLQGHPSTRYMKGIESSSGSLGQGLSVSVGLALGAKLKKETYKIYTCISDGECGEGMTWEAAQ
SAVHFKTDNLIAFMDRNYIQIDGNTEEVMKLEPLDKKFEMFGWNVINADGHNMEEIFSAFAKAKQHTGGPTLIVFRTILG
KGVSYMENNPKWHGTPPNKEQEAQALAELA

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI205277463, Length=269, Percent_Identity=35.3159851301115, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI4507521, Length=269, Percent_Identity=35.3159851301115, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI133778974, Length=270, Percent_Identity=34.8148148148148, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI225637459, Length=267, Percent_Identity=27.3408239700375, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI225637461, Length=267, Percent_Identity=25.8426966292135, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI225637463, Length=230, Percent_Identity=26.5217391304348, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788808, Length=274, Percent_Identity=37.5912408759124, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI48994911, Length=261, Percent_Identity=37.5478927203065, Blast_Score=170, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17539652, Length=247, Percent_Identity=38.0566801619433, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6325331, Length=266, Percent_Identity=37.593984962406, Blast_Score=155, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6319593, Length=264, Percent_Identity=36.7424242424242, Blast_Score=155, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45551847, Length=256, Percent_Identity=37.109375, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI45550715, Length=256, Percent_Identity=37.109375, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24666278, Length=262, Percent_Identity=37.0229007633588, Blast_Score=170, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24645119, Length=217, Percent_Identity=37.7880184331797, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: =2.2.1.1 [H]

Molecular weight: Translated: 29716; Mature: 29716

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00801 TRANSKETOLASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVEVAKKFAKDIRIQVIKMVTAANSGHPGGPLGLADIYAALYTSILNHDPKNPELPER
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DRLILSNGHVCAVRYAAMGLSGYFPVEDLLTFRNINSYLQGHPSTRYMKGIESSSGSLGQ
CEEEEECCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
GLSVSVGLALGAKLKKETYKIYTCISDGECGEGMTWEAAQSAVHFKTDNLIAFMDRNYIQ
CCEEEEHHHHCCHHHCCEEEEEEEECCCCCCCCCCHHHHHCEEEEECCCEEEEECCCEEE
IDGNTEEVMKLEPLDKKFEMFGWNVINADGHNMEEIFSAFAKAKQHTGGPTLIVFRTILG
ECCCHHHHEEECCCCHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KGVSYMENNPKWHGTPPNKEQEAQALAELA
CCCHHHCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MEKVEVAKKFAKDIRIQVIKMVTAANSGHPGGPLGLADIYAALYTSILNHDPKNPELPER
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DRLILSNGHVCAVRYAAMGLSGYFPVEDLLTFRNINSYLQGHPSTRYMKGIESSSGSLGQ
CEEEEECCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
GLSVSVGLALGAKLKKETYKIYTCISDGECGEGMTWEAAQSAVHFKTDNLIAFMDRNYIQ
CCEEEEHHHHCCHHHCCEEEEEEEECCCCCCCCCCHHHHHCEEEEECCCEEEEECCCEEE
IDGNTEEVMKLEPLDKKFEMFGWNVINADGHNMEEIFSAFAKAKQHTGGPTLIVFRTILG
ECCCHHHHEEECCCCHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KGVSYMENNPKWHGTPPNKEQEAQALAELA
CCCHHHCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]