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The map label for this gene is dapF [H]

Identifier: 183220003

GI number: 183220003

Start: 587723

End: 588583

Strand: Direct

Name: dapF [H]

Synonym: LEPBI_I0585

Alternate gene names: 183220003

Gene position: 587723-588583 (Clockwise)

Preceding gene: 183220001

Following gene: 183220006

Centisome position: 16.33

GC content: 44.48

Gene sequence:

>861_bases
GTGAAAAGAGAGGGACCGATGAAAATCAATTTCACCAAAATGGAAGGGATCGGCAATGACTACGTGTACATTGATGCCAC
GAAAAACGATATCCGACTCAGTCCAGAACAAATCCAAAAACTTTCCGATCGTAATTTTGGAATCGGCGGTGATGGTGTGA
TTTTCATTCGAAATTCCAACTCTGGTGAATTCCAAATGGATATGTACAACTCCGATGGAAGTTCCTCTGAGATGTGTGGG
AATGGCGTACGCTGCGTGGGAAAATTTGTCTATGACCATGGTCTTACGAAAAACCAAAAGCCAACCATCGAAACGGGAAA
AGGTGTCCTCACCTTAGATCTGAAAACTGGCACAAATGGAAAAGTGGAAATGGTGACTGTGGATATGGGAGAACCCATCC
TAAAACCATCCCTTGTTCCAATAGTATGGAAAGGGGACGAACCAGTCATCAACCAAGTGATTGAAGTCCAAGGAAAACAA
TACCATTTTACGGCCGTTAGTATGGGGAATCCACATTGTGTGATTTATGTAGACGATGCCGATGATTTTCCAGTGCGCGA
AATTGGACCCATCATTGAAAATCATCCATTCTTCCCGAGACGCGTGAATGTTGAATTTGTATCCATCAAAGGAAAAGACC
ATCTTTACCAAAGGACTTGGGAAAGAGGAACAGGCGAAACCTTGGCTTGCGGAACGGGAGCTTGTGCTGTGACCGTTGCC
TCCATCCTCAATGGCAAAACCGGACGTTCTGTACGAATTGATCTACGTGGTGGAACCCTTCACATCGAATGGCAGGAAAA
TGGATCTGTTTTTATGACAGGCCCCGCCAAAGAAGTGTTCTCTGGAGAAGTAGAGATTTAA

Upstream 100 bases:

>100_bases
GATTTCATGTTCGATTCTGTCAGTCTAATCGTATAGAACAAGGGTCAAACTAAAAATCCTTGATACGGCTTGCCAAGAGC
ATGGGCAAAAAAAACATAGA

Downstream 100 bases:

>100_bases
ATATCTTTAAGCAATCTTTAACAACAGTTGGTTACTTTTTTAAGTAATCAACTTTTTGTAATTCGCCCATTCGCCGTTTG
GTTTCCTCGGAAACGGCCAC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKREGPMKINFTKMEGIGNDYVYIDATKNDIRLSPEQIQKLSDRNFGIGGDGVIFIRNSNSGEFQMDMYNSDGSSSEMCG
NGVRCVGKFVYDHGLTKNQKPTIETGKGVLTLDLKTGTNGKVEMVTVDMGEPILKPSLVPIVWKGDEPVINQVIEVQGKQ
YHFTAVSMGNPHCVIYVDDADDFPVREIGPIIENHPFFPRRVNVEFVSIKGKDHLYQRTWERGTGETLACGTGACAVTVA
SILNGKTGRSVRIDLRGGTLHIEWQENGSVFMTGPAKEVFSGEVEI

Sequences:

>Translated_286_residues
MKREGPMKINFTKMEGIGNDYVYIDATKNDIRLSPEQIQKLSDRNFGIGGDGVIFIRNSNSGEFQMDMYNSDGSSSEMCG
NGVRCVGKFVYDHGLTKNQKPTIETGKGVLTLDLKTGTNGKVEMVTVDMGEPILKPSLVPIVWKGDEPVINQVIEVQGKQ
YHFTAVSMGNPHCVIYVDDADDFPVREIGPIIENHPFFPRRVNVEFVSIKGKDHLYQRTWERGTGETLACGTGACAVTVA
SILNGKTGRSVRIDLRGGTLHIEWQENGSVFMTGPAKEVFSGEVEI
>Mature_286_residues
MKREGPMKINFTKMEGIGNDYVYIDATKNDIRLSPEQIQKLSDRNFGIGGDGVIFIRNSNSGEFQMDMYNSDGSSSEMCG
NGVRCVGKFVYDHGLTKNQKPTIETGKGVLTLDLKTGTNGKVEMVTVDMGEPILKPSLVPIVWKGDEPVINQVIEVQGKQ
YHFTAVSMGNPHCVIYVDDADDFPVREIGPIIENHPFFPRRVNVEFVSIKGKDHLYQRTWERGTGETLACGTGACAVTVA
SILNGKTGRSVRIDLRGGTLHIEWQENGSVFMTGPAKEVFSGEVEI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=37.7224199288256, Blast_Score=213, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31525; Mature: 31525

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREGPMKINFTKMEGIGNDYVYIDATKNDIRLSPEQIQKLSDRNFGIGGDGVIFIRNSN
CCCCCCEEEEEEEECCCCCCEEEEEECCCCEEECHHHHHHHHCCCCCCCCCCEEEEEECC
SGEFQMDMYNSDGSSSEMCGNGVRCVGKFVYDHGLTKNQKPTIETGKGVLTLDLKTGTNG
CCEEEEEEECCCCCCHHHCCCCHHEEEEHHHHCCCCCCCCCCEECCCEEEEEEEECCCCC
KVEMVTVDMGEPILKPSLVPIVWKGDEPVINQVIEVQGKQYHFTAVSMGNPHCVIYVDDA
EEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCEEEEEEECC
DDFPVREIGPIIENHPFFPRRVNVEFVSIKGKDHLYQRTWERGTGETLACGTGACAVTVA
CCCCHHHHCHHHHCCCCCCEEEEEEEEEEECCCHHHHHHHHCCCCCEEEECCCHHHHHHH
SILNGKTGRSVRIDLRGGTLHIEWQENGSVFMTGPAKEVFSGEVEI
HHHCCCCCCEEEEEEECCEEEEEECCCCCEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MKREGPMKINFTKMEGIGNDYVYIDATKNDIRLSPEQIQKLSDRNFGIGGDGVIFIRNSN
CCCCCCEEEEEEEECCCCCCEEEEEECCCCEEECHHHHHHHHCCCCCCCCCCEEEEEECC
SGEFQMDMYNSDGSSSEMCGNGVRCVGKFVYDHGLTKNQKPTIETGKGVLTLDLKTGTNG
CCEEEEEEECCCCCCHHHCCCCHHEEEEHHHHCCCCCCCCCCEECCCEEEEEEEECCCCC
KVEMVTVDMGEPILKPSLVPIVWKGDEPVINQVIEVQGKQYHFTAVSMGNPHCVIYVDDA
EEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCEEEEEEECC
DDFPVREIGPIIENHPFFPRRVNVEFVSIKGKDHLYQRTWERGTGETLACGTGACAVTVA
CCCCHHHHCHHHHCCCCCCEEEEEEEEEEECCCHHHHHHHHCCCCCEEEECCCHHHHHHH
SILNGKTGRSVRIDLRGGTLHIEWQENGSVFMTGPAKEVFSGEVEI
HHHCCCCCCEEEEEEECCEEEEEECCCCCEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA