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The map label for this gene is pheS

Identifier: 183219928

GI number: 183219928

Start: 508461

End: 509486

Strand: Direct

Name: pheS

Synonym: LEPBI_I0510

Alternate gene names: 183219928

Gene position: 508461-509486 (Clockwise)

Preceding gene: 183219927

Following gene: 183219933

Centisome position: 14.13

GC content: 43.47

Gene sequence:

>1026_bases
ATGAGCCTATCCCAAGAAATTGTATCTTTAGTCAAAGAGGCAGAAACTGTATTAAGTTCTGCAACCACTGAACAAGAATT
AGATGCTCTAAAAAACCAGTTCCTTGGTAAAAAAGGAAAACTTACCTCTGTCCTGAAGGGCCTTGCTTCCCTCACAGTCG
AAGAAAAAAAGACGGTTGGGAAAGAGGCAAATGAAGCCCAAACAAAACTGGAACAATTTGTCGAAGCCAAACGGACGATT
CTCAAAGAAAGTTTTTATGAAAACCAATTGGGAAAAGAATCCTTCGATACCCTTCGTCCACTTCCCAAAAAAGAAAGAGG
AAGTTTGCATCCCATTTCCCAAATCCAATACGAGATTGAAGATATTTTTACCTCTATGGGTTTTTCTGTGATGGATGGCC
CGGAAGTGGAAACCGATGAAAACAATTTTGGTGCCTTAAACTTTACCGAAGACCATCCCGCACGGGACATGCAAGATACG
TTTTATACGGTGGATGGCAATTTACTTAGGACTCACACATCCGCCATCCAAGTGCGCGCTTTACGAAAACTAAAACCACC
GTTTCGTATCATTGCTCCAGGCCGTGTGTTCCGGTATGAAGAAGTGGACGCCTCGCATGAAAATACTTTCTACCAAGTGG
AAGGCATGGTTGTGGGCGAAAACATTTCTGTTGCCCATTTGATATACACAATGGAGACACTTTTATCACGAGTGTTCCGA
AAAGAAATCAAAACAAGACTGCGTCCAGGATACTTTCCGTTTGTGGAACCAGGCTTTGAGCTGGATATCAATTGTCTTGT
TTGCAGTGGAGACGGGTGTAGTGTTTGCAAACAATCAGGATGGCTCGAACTCCTTCCTTGTGGACTCGTCCATCCCAATG
TCTTAGAAGCCGCTGGCCTCGACTCCAAAAAGTGGACAGGTTTTGCCTTTGGACTTGGCCTTGACCGTTTGGTGATGATG
CGTTACGGCATCCACGACATCCGTTATTTCCAATCTGGGAATTTACGTTTTTTGAAACAGTTTTAA

Upstream 100 bases:

>100_bases
AATACAAAAAAGAAAAGCTCATCATCCTTGCCATGTCCAATGGTGCCTTTGGTGGGATTTATTCTGAATTAAAATCCTTA
ATGGAAACACGAGAATCACT

Downstream 100 bases:

>100_bases
TTGAGAAGCCAAACAAGGAACCCTTGTTTGGCTGGCTTGTATCTTAACCCAATACAACTGCTTGGATTTTTCCATCGATG
ATTTGGAATTCGACTTTGGT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSLSQEIVSLVKEAETVLSSATTEQELDALKNQFLGKKGKLTSVLKGLASLTVEEKKTVGKEANEAQTKLEQFVEAKRTI
LKESFYENQLGKESFDTLRPLPKKERGSLHPISQIQYEIEDIFTSMGFSVMDGPEVETDENNFGALNFTEDHPARDMQDT
FYTVDGNLLRTHTSAIQVRALRKLKPPFRIIAPGRVFRYEEVDASHENTFYQVEGMVVGENISVAHLIYTMETLLSRVFR
KEIKTRLRPGYFPFVEPGFELDINCLVCSGDGCSVCKQSGWLELLPCGLVHPNVLEAAGLDSKKWTGFAFGLGLDRLVMM
RYGIHDIRYFQSGNLRFLKQF

Sequences:

>Translated_341_residues
MSLSQEIVSLVKEAETVLSSATTEQELDALKNQFLGKKGKLTSVLKGLASLTVEEKKTVGKEANEAQTKLEQFVEAKRTI
LKESFYENQLGKESFDTLRPLPKKERGSLHPISQIQYEIEDIFTSMGFSVMDGPEVETDENNFGALNFTEDHPARDMQDT
FYTVDGNLLRTHTSAIQVRALRKLKPPFRIIAPGRVFRYEEVDASHENTFYQVEGMVVGENISVAHLIYTMETLLSRVFR
KEIKTRLRPGYFPFVEPGFELDINCLVCSGDGCSVCKQSGWLELLPCGLVHPNVLEAAGLDSKKWTGFAFGLGLDRLVMM
RYGIHDIRYFQSGNLRFLKQF
>Mature_340_residues
SLSQEIVSLVKEAETVLSSATTEQELDALKNQFLGKKGKLTSVLKGLASLTVEEKKTVGKEANEAQTKLEQFVEAKRTIL
KESFYENQLGKESFDTLRPLPKKERGSLHPISQIQYEIEDIFTSMGFSVMDGPEVETDENNFGALNFTEDHPARDMQDTF
YTVDGNLLRTHTSAIQVRALRKLKPPFRIIAPGRVFRYEEVDASHENTFYQVEGMVVGENISVAHLIYTMETLLSRVFRK
EIKTRLRPGYFPFVEPGFELDINCLVCSGDGCSVCKQSGWLELLPCGLVHPNVLEAAGLDSKKWTGFAFGLGLDRLVMMR
YGIHDIRYFQSGNLRFLKQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=250, Percent_Identity=32.8, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=29.368029739777, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1788007, Length=335, Percent_Identity=41.1940298507463, Blast_Score=278, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI32563657, Length=295, Percent_Identity=32.2033898305085, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=295, Percent_Identity=32.2033898305085, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=251, Percent_Identity=33.0677290836653, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=368, Percent_Identity=28.804347826087, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=315, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI18858079, Length=283, Percent_Identity=31.8021201413428, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137424, Length=289, Percent_Identity=28.3737024221453, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_LEPBA (B0SBK8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961607.1
- ProteinModelPortal:   B0SBK8
- SMR:   B0SBK8
- GeneID:   6387501
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0490
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   LBIF355278:LBF_0490-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38569; Mature: 38437

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSQEIVSLVKEAETVLSSATTEQELDALKNQFLGKKGKLTSVLKGLASLTVEEKKTVG
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
KEANEAQTKLEQFVEAKRTILKESFYENQLGKESFDTLRPLPKKERGSLHPISQIQYEIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DIFTSMGFSVMDGPEVETDENNFGALNFTEDHPARDMQDTFYTVDGNLLRTHTSAIQVRA
HHHHHCCCEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHH
LRKLKPPFRIIAPGRVFRYEEVDASHENTFYQVEGMVVGENISVAHLIYTMETLLSRVFR
HHHCCCCEEEECCCCEEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
KEIKTRLRPGYFPFVEPGFELDINCLVCSGDGCSVCKQSGWLELLPCGLVHPNVLEAAGL
HHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHCCCEEEECCCCCCCCHHHHCCC
DSKKWTGFAFGLGLDRLVMMRYGIHDIRYFQSGNLRFLKQF
CCCCCCCEEECCCHHHHHHHHHCHHHHHEEECCCEEEEECC
>Mature Secondary Structure 
SLSQEIVSLVKEAETVLSSATTEQELDALKNQFLGKKGKLTSVLKGLASLTVEEKKTVG
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
KEANEAQTKLEQFVEAKRTILKESFYENQLGKESFDTLRPLPKKERGSLHPISQIQYEIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DIFTSMGFSVMDGPEVETDENNFGALNFTEDHPARDMQDTFYTVDGNLLRTHTSAIQVRA
HHHHHCCCEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHH
LRKLKPPFRIIAPGRVFRYEEVDASHENTFYQVEGMVVGENISVAHLIYTMETLLSRVFR
HHHCCCCEEEECCCCEEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
KEIKTRLRPGYFPFVEPGFELDINCLVCSGDGCSVCKQSGWLELLPCGLVHPNVLEAAGL
HHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHCCCEEEECCCCCCCCHHHHCCC
DSKKWTGFAFGLGLDRLVMMRYGIHDIRYFQSGNLRFLKQF
CCCCCCCEEECCCHHHHHHHHHCHHHHHEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA