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The map label for this gene is mpl [H]

Identifier: 183219927

GI number: 183219927

Start: 507070

End: 508464

Strand: Direct

Name: mpl [H]

Synonym: LEPBI_I0509

Alternate gene names: 183219927

Gene position: 507070-508464 (Clockwise)

Preceding gene: 183219926

Following gene: 183219928

Centisome position: 14.09

GC content: 40.07

Gene sequence:

>1395_bases
TTGAAGATCTTTTTGGTAGGAATTGGTGGGATTGCCATGGGCAATTTAGCCTATATGTTGAAACAACAAGGTCATGAAGT
ATCTGGTTCAGACCAAAACCTATACCCACCGATGTCTGATAAATTGTTAGAGTGGGGTCTTTCACCTAAGTCTGGTTATC
GTAAAGAAAATGTGAAAGGTGCAGACCTTGTCATCATTGGAAATGCGATCTCACGTGGGAATCCTGAAGTCGAAGAAGTT
TTGAATACGGGAATTGAATACATGAGTATGGCAGAAGCGATTGGACATTTTTTCCTCAAAGGGAAAAAACCAATCGTGAT
CTCAGGAACCCATGGCAAAACCACCACTACCTTTCTGACTCATTGGATTTTGGAATCGATTGGACTGAGACCAGGTCTTT
TTGTAGGTGGAATCCGTAAGGATGGATTTCCTGGATTTGCTCTAGGTGAAGGGGATTATTTTGTGATTGAAGGGGATGAA
TATGATTCTGCTTTCTTTGATAAAAGTTCCAAGTTTTTACATTACAGACCCTATTACCTGGTGATGAATGCACTTGACTT
TGACCATGCTGACATCTTTGCCAATTTGGATGCGATTAAAGTCATGTTCAAACGGCTGTTAAATTTAGTTCCGAGTCGTG
GAAAGGTATTTTATTGGAAAGGCTCCAAAAATTTAAACGATATCACAAAAGACTACCAGCACGCTCCCTTGGAAACCTTT
GAACTAGGTGATAAAAATTCCATTTTTAAATATGAAAAAGGAATTTTAACGGAGATCCGCACAAAGGCGAAACTCAAACC
ATCTCTCATCGGGTCACATAACTATCGTAATGTGGAAGTCGCCGTGCGAGTGTGCCTAGAAATTGCTCCCCAAAAACGAA
AAGAAATTTTGGAAGCCGTGGAATCCTTTCCTGGTGTGAAACGGAGACAAGAAAACCTTTATGTTTCCGAAACCAGTTTA
CTTGTGGAAGACTTTGCCCACCACCCAGTAGCCATCCAAGAAACGATCAAAGCCCACAAAGAAGCTTACCCTGGTTACAA
AATCATCGCTTTATTCGAACCGAGGAGTGCCACCTCACATAGAAATGTCTTCCAAGATGATTTTGCAAAGTGTTTCAAAG
GAAGTGATGTCAGCATCGTCACCGAAGTGTACCAGGTGGATAAAGTGAGTAAGTCACTTCGTCTCAATGTCAAAAAATTG
GTAAAAGACATCAAAACCAATACCAAAAAAGAATCGATCTATGCTTCCACACCAAAAGAGATTCCTTCCCTTTTAAAAAA
GATATTACCCAAATACAAAAAAGAAAAGCTCATCATCCTTGCCATGTCCAATGGTGCCTTTGGTGGGATTTATTCTGAAT
TAAAATCCTTAATGGAAACACGAGAATCACTATGA

Upstream 100 bases:

>100_bases
CGTTCGATTTTTTATTCTTTTTGATGAAAGTTGTCTTGTTCTTTTTGGTTCTGCTGTCGATTCCATTATTCCTTTATAAC
CTCGTTAGGACGGTACTCTT

Downstream 100 bases:

>100_bases
GCCTATCCCAAGAAATTGTATCTTTAGTCAAAGAGGCAGAAACTGTATTAAGTTCTGCAACCACTGAACAAGAATTAGAT
GCTCTAAAAAACCAGTTCCT

Product: UDP-n-acetylmuramate:l-alanyl-gamma-d-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKIFLVGIGGIAMGNLAYMLKQQGHEVSGSDQNLYPPMSDKLLEWGLSPKSGYRKENVKGADLVIIGNAISRGNPEVEEV
LNTGIEYMSMAEAIGHFFLKGKKPIVISGTHGKTTTTFLTHWILESIGLRPGLFVGGIRKDGFPGFALGEGDYFVIEGDE
YDSAFFDKSSKFLHYRPYYLVMNALDFDHADIFANLDAIKVMFKRLLNLVPSRGKVFYWKGSKNLNDITKDYQHAPLETF
ELGDKNSIFKYEKGILTEIRTKAKLKPSLIGSHNYRNVEVAVRVCLEIAPQKRKEILEAVESFPGVKRRQENLYVSETSL
LVEDFAHHPVAIQETIKAHKEAYPGYKIIALFEPRSATSHRNVFQDDFAKCFKGSDVSIVTEVYQVDKVSKSLRLNVKKL
VKDIKTNTKKESIYASTPKEIPSLLKKILPKYKKEKLIILAMSNGAFGGIYSELKSLMETRESL

Sequences:

>Translated_464_residues
MKIFLVGIGGIAMGNLAYMLKQQGHEVSGSDQNLYPPMSDKLLEWGLSPKSGYRKENVKGADLVIIGNAISRGNPEVEEV
LNTGIEYMSMAEAIGHFFLKGKKPIVISGTHGKTTTTFLTHWILESIGLRPGLFVGGIRKDGFPGFALGEGDYFVIEGDE
YDSAFFDKSSKFLHYRPYYLVMNALDFDHADIFANLDAIKVMFKRLLNLVPSRGKVFYWKGSKNLNDITKDYQHAPLETF
ELGDKNSIFKYEKGILTEIRTKAKLKPSLIGSHNYRNVEVAVRVCLEIAPQKRKEILEAVESFPGVKRRQENLYVSETSL
LVEDFAHHPVAIQETIKAHKEAYPGYKIIALFEPRSATSHRNVFQDDFAKCFKGSDVSIVTEVYQVDKVSKSLRLNVKKL
VKDIKTNTKKESIYASTPKEIPSLLKKILPKYKKEKLIILAMSNGAFGGIYSELKSLMETRESL
>Mature_464_residues
MKIFLVGIGGIAMGNLAYMLKQQGHEVSGSDQNLYPPMSDKLLEWGLSPKSGYRKENVKGADLVIIGNAISRGNPEVEEV
LNTGIEYMSMAEAIGHFFLKGKKPIVISGTHGKTTTTFLTHWILESIGLRPGLFVGGIRKDGFPGFALGEGDYFVIEGDE
YDSAFFDKSSKFLHYRPYYLVMNALDFDHADIFANLDAIKVMFKRLLNLVPSRGKVFYWKGSKNLNDITKDYQHAPLETF
ELGDKNSIFKYEKGILTEIRTKAKLKPSLIGSHNYRNVEVAVRVCLEIAPQKRKEILEAVESFPGVKRRQENLYVSETSL
LVEDFAHHPVAIQETIKAHKEAYPGYKIIALFEPRSATSHRNVFQDDFAKCFKGSDVSIVTEVYQVDKVSKSLRLNVKKL
VKDIKTNTKKESIYASTPKEIPSLLKKILPKYKKEKLIILAMSNGAFGGIYSELKSLMETRESL

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=467, Percent_Identity=36.830835117773, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1786279, Length=470, Percent_Identity=25.9574468085106, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 52240; Mature: 52240

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFLVGIGGIAMGNLAYMLKQQGHEVSGSDQNLYPPMSDKLLEWGLSPKSGYRKENVKG
CEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
ADLVIIGNAISRGNPEVEEVLNTGIEYMSMAEAIGHFFLKGKKPIVISGTHGKTTTTFLT
CCEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHH
HWILESIGLRPGLFVGGIRKDGFPGFALGEGDYFVIEGDEYDSAFFDKSSKFLHYRPYYL
HHHHHHHCCCCCEEECCCCCCCCCCEEECCCCEEEEECCCCCHHHHCCCCCEEEECCCEE
VMNALDFDHADIFANLDAIKVMFKRLLNLVPSRGKVFYWKGSKNLNDITKDYQHAPLETF
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCEE
ELGDKNSIFKYEKGILTEIRTKAKLKPSLIGSHNYRNVEVAVRVCLEIAPQKRKEILEAV
ECCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
ESFPGVKRRQENLYVSETSLLVEDFAHHPVAIQETIKAHKEAYPGYKIIALFEPRSATSH
HHCCCCHHHHCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
RNVFQDDFAKCFKGSDVSIVTEVYQVDKVSKSLRLNVKKLVKDIKTNTKKESIYASTPKE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHH
IPSLLKKILPKYKKEKLIILAMSNGAFGGIYSELKSLMETRESL
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIFLVGIGGIAMGNLAYMLKQQGHEVSGSDQNLYPPMSDKLLEWGLSPKSGYRKENVKG
CEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
ADLVIIGNAISRGNPEVEEVLNTGIEYMSMAEAIGHFFLKGKKPIVISGTHGKTTTTFLT
CCEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHH
HWILESIGLRPGLFVGGIRKDGFPGFALGEGDYFVIEGDEYDSAFFDKSSKFLHYRPYYL
HHHHHHHCCCCCEEECCCCCCCCCCEEECCCCEEEEECCCCCHHHHCCCCCEEEECCCEE
VMNALDFDHADIFANLDAIKVMFKRLLNLVPSRGKVFYWKGSKNLNDITKDYQHAPLETF
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCEE
ELGDKNSIFKYEKGILTEIRTKAKLKPSLIGSHNYRNVEVAVRVCLEIAPQKRKEILEAV
ECCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
ESFPGVKRRQENLYVSETSLLVEDFAHHPVAIQETIKAHKEAYPGYKIIALFEPRSATSH
HHCCCCHHHHCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
RNVFQDDFAKCFKGSDVSIVTEVYQVDKVSKSLRLNVKKLVKDIKTNTKKESIYASTPKE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHH
IPSLLKKILPKYKKEKLIILAMSNGAFGGIYSELKSLMETRESL
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]