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The map label for this gene is algI [H]

Identifier: 183219734

GI number: 183219734

Start: 308934

End: 310490

Strand: Direct

Name: algI [H]

Synonym: LEPBI_I0310

Alternate gene names: 183219734

Gene position: 308934-310490 (Clockwise)

Preceding gene: 183219724

Following gene: 183219735

Centisome position: 8.58

GC content: 38.6

Gene sequence:

>1557_bases
ATGCTTTTTAACACTTTCGTCTTTTTATTATTCTTTCTCATCGTGTATTCGGTTTTTTTGGGTTTTGGATGGTTTGCCAA
AAAACAGAAATGGGCCTACCGTGCTCAAAACCTTTGGTTACTCTTAGCCTCCTACTTTTTTTATGGGTGGTGGGAATGGT
TTTTCCTTACCCTCATTTTAGTCAGTACCATCATTGATTACATCGCAGCAAGGCTCATTGAATCTTCTCAACATCAAATC
CGTCGAAGGATCTATCTATCAGTTTCGATTATAGCCAATTTGGGCCTACTGTTCGTAATGAAGTATTATGATTTTTTTGC
AGTGAATCTGATTGATTCTTGGAACCAAATGTCCATTTGGCTAGGTGGAACGGCTGCCACAGATTCGAGTACTTATCTTT
TGAAAAACATCGTACTCCCTGTCGGGATTAGTTTTTATACCTTCCAAACTATGTCCTACACCATCGATGTGTACAGAAAA
CAAATCCAAGCAGAGCGTGATTTTTTTGACTTCGCTTTGTTTGTGAATTTTTTCCCTCAGCTCGTGGCGGGGCCCATAGA
AAGGGCACAAGACCTACTCCCACAATTGAAACAACCTAAGTTCCCCACAATAGAAGGCGTACAAAAAGGTTTGTATGACA
TTTTACTTGGTTACTTTATGAAAGTGTATGTCGCTGATAATTTAGCTACGTATGTGGACCAAGTATTTTTTGCTGGGAAA
TCTTTTTATGTGCAAAATCCGGAAATCATACATTCGGTAGACGGCTCACAAGTGTTCGCTGGTGGTTTTTTATTTTTAAC
TCAGATATATTGTGACTTTGCTGGTTATTCTTTCATAGCGCTTGGAATCTCTCGTTTACTTGGTGTGACACTCACCGTGA
ATTTTGAAACACCAGAATTTTCCAAAACTCCAACGGAATTCTGGAATCGATGGCACGTAACATTGAATCGTTGGTTTCGC
GATTATATTTATATCTCTCTTGGGGGAAGTAAATACGGTAAATTTGCGCAATACAGAAATTTATGTATCATCTTTTTCCT
TTCAGGGCTTTGGCATGGTGCCAATTGGACCTTCATCACTTGGGGTTGTTTGCAAGGAGTGTATACCATTATCTATTTAG
TGGGATTTTCCAAAAAGAAAGAAGACCTAGGTGAGAATCCAAACGAATCCACTCACTCCATTTCTTCCCAGTTTATCCGA
ATCCTTTCTGGGTCCTTCAGTCGCGTATTGGTGTATGCGTTGGTTGCCTTTAGTGCGGTTGCTTTTCGTTCCTATGATGC
AAACATGATGTTAGTGTACTTCCAAAAATTCCTTTCGTTTTGGACTTGGGATTTGTCTCCAAATAATAACGTCAAAAATA
TGTTTGGACTTTGGGAAGAATATTTTAAAATCTTTCTACCACTCCTAATCATTGATGGGATCACCTATTTCAAAAAAGAA
AGGTATTGGTTGTTTTTAACACATCCACTCATCCAAGCAGTTGTTTTATCTTTTATGGCATTTCTCATCCTCACCAGAGG
AGTTTTTGGAAAAGAGGTAATTTACTTTGCGTTCTAA

Upstream 100 bases:

>100_bases
TTTTTGGGAAATAGGAACGGGTCAACGATAAGATTCTCTCTTTTTTACGCATTTTTGAATTTTACAGAATCCCATGACCT
TGAAATTGTAACGAAGACCG

Downstream 100 bases:

>100_bases
ATTTTTAGGCATCGGACTCGCTCTTTTGTTTTTTTTGGTTTTGGAAATCACAGTCCGTGTGACAGGCATTCATTATTTGG
AACAACCTGAAATCTTCTTT

Product: putative poly(beta-D-mannuronate) O-acetylase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MLFNTFVFLLFFLIVYSVFLGFGWFAKKQKWAYRAQNLWLLLASYFFYGWWEWFFLTLILVSTIIDYIAARLIESSQHQI
RRRIYLSVSIIANLGLLFVMKYYDFFAVNLIDSWNQMSIWLGGTAATDSSTYLLKNIVLPVGISFYTFQTMSYTIDVYRK
QIQAERDFFDFALFVNFFPQLVAGPIERAQDLLPQLKQPKFPTIEGVQKGLYDILLGYFMKVYVADNLATYVDQVFFAGK
SFYVQNPEIIHSVDGSQVFAGGFLFLTQIYCDFAGYSFIALGISRLLGVTLTVNFETPEFSKTPTEFWNRWHVTLNRWFR
DYIYISLGGSKYGKFAQYRNLCIIFFLSGLWHGANWTFITWGCLQGVYTIIYLVGFSKKKEDLGENPNESTHSISSQFIR
ILSGSFSRVLVYALVAFSAVAFRSYDANMMLVYFQKFLSFWTWDLSPNNNVKNMFGLWEEYFKIFLPLLIIDGITYFKKE
RYWLFLTHPLIQAVVLSFMAFLILTRGVFGKEVIYFAF

Sequences:

>Translated_518_residues
MLFNTFVFLLFFLIVYSVFLGFGWFAKKQKWAYRAQNLWLLLASYFFYGWWEWFFLTLILVSTIIDYIAARLIESSQHQI
RRRIYLSVSIIANLGLLFVMKYYDFFAVNLIDSWNQMSIWLGGTAATDSSTYLLKNIVLPVGISFYTFQTMSYTIDVYRK
QIQAERDFFDFALFVNFFPQLVAGPIERAQDLLPQLKQPKFPTIEGVQKGLYDILLGYFMKVYVADNLATYVDQVFFAGK
SFYVQNPEIIHSVDGSQVFAGGFLFLTQIYCDFAGYSFIALGISRLLGVTLTVNFETPEFSKTPTEFWNRWHVTLNRWFR
DYIYISLGGSKYGKFAQYRNLCIIFFLSGLWHGANWTFITWGCLQGVYTIIYLVGFSKKKEDLGENPNESTHSISSQFIR
ILSGSFSRVLVYALVAFSAVAFRSYDANMMLVYFQKFLSFWTWDLSPNNNVKNMFGLWEEYFKIFLPLLIIDGITYFKKE
RYWLFLTHPLIQAVVLSFMAFLILTRGVFGKEVIYFAF
>Mature_518_residues
MLFNTFVFLLFFLIVYSVFLGFGWFAKKQKWAYRAQNLWLLLASYFFYGWWEWFFLTLILVSTIIDYIAARLIESSQHQI
RRRIYLSVSIIANLGLLFVMKYYDFFAVNLIDSWNQMSIWLGGTAATDSSTYLLKNIVLPVGISFYTFQTMSYTIDVYRK
QIQAERDFFDFALFVNFFPQLVAGPIERAQDLLPQLKQPKFPTIEGVQKGLYDILLGYFMKVYVADNLATYVDQVFFAGK
SFYVQNPEIIHSVDGSQVFAGGFLFLTQIYCDFAGYSFIALGISRLLGVTLTVNFETPEFSKTPTEFWNRWHVTLNRWFR
DYIYISLGGSKYGKFAQYRNLCIIFFLSGLWHGANWTFITWGCLQGVYTIIYLVGFSKKKEDLGENPNESTHSISSQFIR
ILSGSFSRVLVYALVAFSAVAFRSYDANMMLVYFQKFLSFWTWDLSPNNNVKNMFGLWEEYFKIFLPLLIIDGITYFKKE
RYWLFLTHPLIQAVVLSFMAFLILTRGVFGKEVIYFAF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 60609; Mature: 60609

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
>Mature Secondary Structure
MLFNTFVFLLFFLIVYSVFLGFGWFAKKQKWAYRAQNLWLLLASYFFYGWWEWFFLTLIL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSTIIDYIAARLIESSQHQIRRRIYLSVSIIANLGLLFVMKYYDFFAVNLIDSWNQMSIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LGGTAATDSSTYLLKNIVLPVGISFYTFQTMSYTIDVYRKQIQAERDFFDFALFVNFFPQ
ECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAGPIERAQDLLPQLKQPKFPTIEGVQKGLYDILLGYFMKVYVADNLATYVDQVFFAGK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SFYVQNPEIIHSVDGSQVFAGGFLFLTQIYCDFAGYSFIALGISRLLGVTLTVNFETPEF
CEEECCCHHEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEEEECCCCC
SKTPTEFWNRWHVTLNRWFRDYIYISLGGSKYGKFAQYRNLCIIFFLSGLWHGANWTFIT
CCCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
WGCLQGVYTIIYLVGFSKKKEDLGENPNESTHSISSQFIRILSGSFSRVLVYALVAFSAV
HHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AFRSYDANMMLVYFQKFLSFWTWDLSPNNNVKNMFGLWEEYFKIFLPLLIIDGITYFKKE
HHHCCCCCHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RYWLFLTHPLIQAVVLSFMAFLILTRGVFGKEVIYFAF
CEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]