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The map label for this gene is anfA [H]

Identifier: 183219679

GI number: 183219679

Start: 245072

End: 246568

Strand: Direct

Name: anfA [H]

Synonym: LEPBI_I0254

Alternate gene names: 183219679

Gene position: 245072-246568 (Clockwise)

Preceding gene: 183219678

Following gene: 183219680

Centisome position: 6.81

GC content: 39.68

Gene sequence:

>1497_bases
ATGTCTGTAAAACAGGATATTTCCGGCACTTTAAGGAAAATCCAAAAAGAAATCCAACAACTTCCGAATATTACGGATCG
ATTGAATTTCATTTTGGATATGACTCTTACCCTTTTTGGTGCTTCAACTGGAAGTATCTCCATCATGGACCAAGAAGAAA
AGGTTCTTACCATTGTCGCGGCCAAAGGTATGGACTGGGAGAAAAAAATTGCCGCAAAACTTCCGTTTAATTTAGGTGTT
ACGGGACGAGCCGCTTCCTCAAGAGAAATCATCTATGTGCCTGATGTTACTTTGGACAAAGACTATGTAAAACTCATTGA
AACAGTTCGTTCGGAACTTGCCATTCCACTTCTCACTAGAGATTCGACAGTCGGAGTCCTCAATTTAGAATCTGACAAAG
TTAACTTTTTTTCACCCGACATCATCAACCAAGCCACCCTATTTGCTTCACAACTAACGATTGTCATTCTAGAAGAAAGA
ATCGCAAAAGAAGCATTTGAAAAATCCAAACGAGAAGAAGACCCAGTGGAAGAAGTACTTGGTTATGATCCGAGTATTTT
GTTTTTAAAACACCGGATTCGTCAAGTGGGTCCTTCTGATATTTCTGTTATGATTATTGGAGAAGAAGGATCAGGAAAGA
AGTTAGTGGCTAAGGCATTACATTTCATTTCGCAAAGAAAAAATGCTCCATTTTCAACCGTAGATTGTTCGGGTCTTAGT
TATGAATTATTGGAAGCTGAACTCTTCGGAAGTTTTAGCGGAAAAATATTCAATCCAGGAAAATTAGAACAATCCAATGG
TGGCTCCTTATACATTGAATCCATTGGAGATCTTCCTCCCAATTTACAAACAAAACTCTTTCAAATATTACGAGATAAAA
CCATCCCAAACCCAACTTCCAAAAAAAAAGAAGAAGTGTTGAACATTCGTATTTTTACAGGGAGTAAACGAGATTTATTA
GAAGACATCCAAAAAGAAACCTTCTCTATGGATTTGTATTACCGACTAGCAGAAGTTCCACTTCGTGTGCCACCTTTGCG
TGAACGAAGAGGAGACATTCCACTCCTTGCCCATCATTATTTATACCAATACAACAAACAATACGGAAGGAACAAATCGT
TTTCAACAGATGCACTCAAGGCACTCACAGGGATGCCGTGGAGTGGCAATGTGCGCCAACTGCAAAGTGTCATCCAGTAT
GCAGTCCTTGTCCCACAAGAAACGGTTCTTGAACCATATTCCTTTCAACAAGATGGAAAACGCGAAGAGGAGTCTCACCA
AAAAGTTTCAAGATTCGGTGAAGGCATGGAAGTTCTTGCACCAAGTGAGAATTTGTCTTTGAACTTAGCGATTGAAAGAC
TTGAGGCAATTTGGATCAAAGAAGCCTTCCAAAGGGCCTCAACACAAGAAGAGGTCGCAAAACTTTTGGGGATCAGCCGT
GGTTCCTTGCAATATAAACTCAAAAATAACCAATTTCTGGACGGTTTTAGCACCTAA

Upstream 100 bases:

>100_bases
GGTAATGTCCACGCAGTGATGGATGGAGACTTGGAAGATTTTATAATCGCTTATTTAAAATACCTGACAAATCAAAAGGC
AAACGCTAAAGTATAACCCC

Downstream 100 bases:

>100_bases
TCACCAGAAAAGAATGGATCGAACGGCCTCTTTGGTCGATACTACTAGAAAGGAGCTTCGAAGTGGCAGAAAGTTATTAC
CGTACCATTAGTGGTAAACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSVKQDISGTLRKIQKEIQQLPNITDRLNFILDMTLTLFGASTGSISIMDQEEKVLTIVAAKGMDWEKKIAAKLPFNLGV
TGRAASSREIIYVPDVTLDKDYVKLIETVRSELAIPLLTRDSTVGVLNLESDKVNFFSPDIINQATLFASQLTIVILEER
IAKEAFEKSKREEDPVEEVLGYDPSILFLKHRIRQVGPSDISVMIIGEEGSGKKLVAKALHFISQRKNAPFSTVDCSGLS
YELLEAELFGSFSGKIFNPGKLEQSNGGSLYIESIGDLPPNLQTKLFQILRDKTIPNPTSKKKEEVLNIRIFTGSKRDLL
EDIQKETFSMDLYYRLAEVPLRVPPLRERRGDIPLLAHHYLYQYNKQYGRNKSFSTDALKALTGMPWSGNVRQLQSVIQY
AVLVPQETVLEPYSFQQDGKREEESHQKVSRFGEGMEVLAPSENLSLNLAIERLEAIWIKEAFQRASTQEEVAKLLGISR
GSLQYKLKNNQFLDGFST

Sequences:

>Translated_498_residues
MSVKQDISGTLRKIQKEIQQLPNITDRLNFILDMTLTLFGASTGSISIMDQEEKVLTIVAAKGMDWEKKIAAKLPFNLGV
TGRAASSREIIYVPDVTLDKDYVKLIETVRSELAIPLLTRDSTVGVLNLESDKVNFFSPDIINQATLFASQLTIVILEER
IAKEAFEKSKREEDPVEEVLGYDPSILFLKHRIRQVGPSDISVMIIGEEGSGKKLVAKALHFISQRKNAPFSTVDCSGLS
YELLEAELFGSFSGKIFNPGKLEQSNGGSLYIESIGDLPPNLQTKLFQILRDKTIPNPTSKKKEEVLNIRIFTGSKRDLL
EDIQKETFSMDLYYRLAEVPLRVPPLRERRGDIPLLAHHYLYQYNKQYGRNKSFSTDALKALTGMPWSGNVRQLQSVIQY
AVLVPQETVLEPYSFQQDGKREEESHQKVSRFGEGMEVLAPSENLSLNLAIERLEAIWIKEAFQRASTQEEVAKLLGISR
GSLQYKLKNNQFLDGFST
>Mature_497_residues
SVKQDISGTLRKIQKEIQQLPNITDRLNFILDMTLTLFGASTGSISIMDQEEKVLTIVAAKGMDWEKKIAAKLPFNLGVT
GRAASSREIIYVPDVTLDKDYVKLIETVRSELAIPLLTRDSTVGVLNLESDKVNFFSPDIINQATLFASQLTIVILEERI
AKEAFEKSKREEDPVEEVLGYDPSILFLKHRIRQVGPSDISVMIIGEEGSGKKLVAKALHFISQRKNAPFSTVDCSGLSY
ELLEAELFGSFSGKIFNPGKLEQSNGGSLYIESIGDLPPNLQTKLFQILRDKTIPNPTSKKKEEVLNIRIFTGSKRDLLE
DIQKETFSMDLYYRLAEVPLRVPPLRERRGDIPLLAHHYLYQYNKQYGRNKSFSTDALKALTGMPWSGNVRQLQSVIQYA
VLVPQETVLEPYSFQQDGKREEESHQKVSRFGEGMEVLAPSENLSLNLAIERLEAIWIKEAFQRASTQEEVAKLLGISRG
SLQYKLKNNQFLDGFST

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789233, Length=339, Percent_Identity=32.7433628318584, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=39.1666666666667, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=31.8611987381703, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788550, Length=318, Percent_Identity=35.2201257861635, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082117, Length=315, Percent_Identity=32.6984126984127, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1790437, Length=232, Percent_Identity=34.9137931034483, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=32.3262839879154, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI87082152, Length=237, Percent_Identity=36.7088607594937, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI87081872, Length=233, Percent_Identity=31.7596566523605, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787583, Length=332, Percent_Identity=27.4096385542169, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=28.9808917197452, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786524, Length=288, Percent_Identity=30.5555555555556, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789828, Length=210, Percent_Identity=25.2380952380952, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56128; Mature: 55997

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKQDISGTLRKIQKEIQQLPNITDRLNFILDMTLTLFGASTGSISIMDQEEKVLTIVA
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEE
AKGMDWEKKIAAKLPFNLGVTGRAASSREIIYVPDVTLDKDYVKLIETVRSELAIPLLTR
ECCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEC
DSTVGVLNLESDKVNFFSPDIINQATLFASQLTIVILEERIAKEAFEKSKREEDPVEEVL
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GYDPSILFLKHRIRQVGPSDISVMIIGEEGSGKKLVAKALHFISQRKNAPFSTVDCSGLS
CCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCC
YELLEAELFGSFSGKIFNPGKLEQSNGGSLYIESIGDLPPNLQTKLFQILRDKTIPNPTS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
KKKEEVLNIRIFTGSKRDLLEDIQKETFSMDLYYRLAEVPLRVPPLRERRGDIPLLAHHY
HHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHH
LYQYNKQYGRNKSFSTDALKALTGMPWSGNVRQLQSVIQYAVLVPQETVLEPYSFQQDGK
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCC
REEESHQKVSRFGEGMEVLAPSENLSLNLAIERLEAIWIKEAFQRASTQEEVAKLLGISR
CCHHHHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
GSLQYKLKNNQFLDGFST
CCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SVKQDISGTLRKIQKEIQQLPNITDRLNFILDMTLTLFGASTGSISIMDQEEKVLTIVA
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEE
AKGMDWEKKIAAKLPFNLGVTGRAASSREIIYVPDVTLDKDYVKLIETVRSELAIPLLTR
ECCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEC
DSTVGVLNLESDKVNFFSPDIINQATLFASQLTIVILEERIAKEAFEKSKREEDPVEEVL
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GYDPSILFLKHRIRQVGPSDISVMIIGEEGSGKKLVAKALHFISQRKNAPFSTVDCSGLS
CCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCC
YELLEAELFGSFSGKIFNPGKLEQSNGGSLYIESIGDLPPNLQTKLFQILRDKTIPNPTS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
KKKEEVLNIRIFTGSKRDLLEDIQKETFSMDLYYRLAEVPLRVPPLRERRGDIPLLAHHY
HHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHH
LYQYNKQYGRNKSFSTDALKALTGMPWSGNVRQLQSVIQYAVLVPQETVLEPYSFQQDGK
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCC
REEESHQKVSRFGEGMEVLAPSENLSLNLAIERLEAIWIKEAFQRASTQEEVAKLLGISR
CCHHHHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
GSLQYKLKNNQFLDGFST
CCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]