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The map label for this gene is prfB [H]

Identifier: 183219678

GI number: 183219678

Start: 243940

End: 245067

Strand: Direct

Name: prfB [H]

Synonym: LEPBI_I0253

Alternate gene names: 183219678

Gene position: 243940-245067 (Clockwise)

Preceding gene: 183219677

Following gene: 183219679

Centisome position: 6.78

GC content: 38.74

Gene sequence:

>1128_bases
ATGGATAGATCATTAAAAGAATTAAAAAAACAAACTACTGAGATGATAGAATCATTTCAAACCTATTGGACCGCACAAAA
TTTCCAAGAGGATTACGATCGGTTATTGTCACTCATTGAAAAAGCAAACGATCCCAAGTTATGGGATTCACCTGACCAAG
CAAAAAACGTTACACAAAAACGTAACGAACTTCAAATGAAATTGGATCCTTGGTTAGGGTTAAAAAAAGAATTATTGGAT
TTTCCTGACTTGATTGAACTTACATCGGAAGAGATGGGTGAAAGTGGTTTAAAATCTTTAAACGATGATTTTGATCGTAT
GTTTGAATCATTTGAAAACTTGCAAATGTTAGATGCACTTTCAGGAAAAGATGATGGAAAGGCAGCCTTTATCAACATTC
ACCCTGGAGCTGGTGGAACTGAGTCACAGGACTGGGCTGATATGTTACTACGAATGTATACTCGGTTCGCGGAACAAAAA
GGATATCGTGCTGAACTTGTGGACTACCAACCAGGTGAAACAGCGGGAATCAAAAACGCCACTCTTTATATTCAAGGTGA
TCATCCCTTTGGGTATTTAAAATGTGAATCAGGAGTCCATCGATTGGTACGAATTTCACCGTTTGATTCCAATAAACGGA
GACATACCTCTTTTGCATCCGTGTATGTCACTCCAGAAGTCGATGATGATATCCAAGTCAATATTGAAGAAAAAGACTTA
CGAGTGGATGTGTATCGATCTTCAGGAGCTGGTGGTCAGCACGTCAACACAACAGACTCTGCCGTTCGAATCACCCACAT
ACCTACAGGGGTAGTTGTTTCCTGTCAGATGGAAAGATCCCAAATCAAAAACCGTGATACAGCTATGAAGATGTTGAAAG
CACGGCTTTATGAGATGGAAAAACAAAAAGCCGAAGAGGAAAATGCTAAAAAAGCCGGTGAAAAACGTGATATTGCATGG
GGTTCACAAATTCGAAGTTATGTGTTCCATCCTTATAATTTGGTAAAAGACCATCGCACAGATTTTGAAACAGGTAATGT
CCACGCAGTGATGGATGGAGACTTGGAAGATTTTATAATCGCTTATTTAAAATACCTGACAAATCAAAAGGCAAACGCTA
AAGTATAA

Upstream 100 bases:

>100_bases
AAGGGAATACACCGCAACTACGAGAAACAACATTCCAATGGGAAGTGGAACGTTTGCGTAAGGATTCTTTGTTTTAATAT
ACAATTCGGTTTTACACATT

Downstream 100 bases:

>100_bases
CCCCATGTCTGTAAAACAGGATATTTCCGGCACTTTAAGGAAAATCCAAAAAGAAATCCAACAACTTCCGAATATTACGG
ATCGATTGAATTTCATTTTG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MDRSLKELKKQTTEMIESFQTYWTAQNFQEDYDRLLSLIEKANDPKLWDSPDQAKNVTQKRNELQMKLDPWLGLKKELLD
FPDLIELTSEEMGESGLKSLNDDFDRMFESFENLQMLDALSGKDDGKAAFINIHPGAGGTESQDWADMLLRMYTRFAEQK
GYRAELVDYQPGETAGIKNATLYIQGDHPFGYLKCESGVHRLVRISPFDSNKRRHTSFASVYVTPEVDDDIQVNIEEKDL
RVDVYRSSGAGGQHVNTTDSAVRITHIPTGVVVSCQMERSQIKNRDTAMKMLKARLYEMEKQKAEEENAKKAGEKRDIAW
GSQIRSYVFHPYNLVKDHRTDFETGNVHAVMDGDLEDFIIAYLKYLTNQKANAKV

Sequences:

>Translated_375_residues
MDRSLKELKKQTTEMIESFQTYWTAQNFQEDYDRLLSLIEKANDPKLWDSPDQAKNVTQKRNELQMKLDPWLGLKKELLD
FPDLIELTSEEMGESGLKSLNDDFDRMFESFENLQMLDALSGKDDGKAAFINIHPGAGGTESQDWADMLLRMYTRFAEQK
GYRAELVDYQPGETAGIKNATLYIQGDHPFGYLKCESGVHRLVRISPFDSNKRRHTSFASVYVTPEVDDDIQVNIEEKDL
RVDVYRSSGAGGQHVNTTDSAVRITHIPTGVVVSCQMERSQIKNRDTAMKMLKARLYEMEKQKAEEENAKKAGEKRDIAW
GSQIRSYVFHPYNLVKDHRTDFETGNVHAVMDGDLEDFIIAYLKYLTNQKANAKV
>Mature_375_residues
MDRSLKELKKQTTEMIESFQTYWTAQNFQEDYDRLLSLIEKANDPKLWDSPDQAKNVTQKRNELQMKLDPWLGLKKELLD
FPDLIELTSEEMGESGLKSLNDDFDRMFESFENLQMLDALSGKDDGKAAFINIHPGAGGTESQDWADMLLRMYTRFAEQK
GYRAELVDYQPGETAGIKNATLYIQGDHPFGYLKCESGVHRLVRISPFDSNKRRHTSFASVYVTPEVDDDIQVNIEEKDL
RVDVYRSSGAGGQHVNTTDSAVRITHIPTGVVVSCQMERSQIKNRDTAMKMLKARLYEMEKQKAEEENAKKAGEKRDIAW
GSQIRSYVFHPYNLVKDHRTDFETGNVHAVMDGDLEDFIIAYLKYLTNQKANAKV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=255, Percent_Identity=40, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI34577120, Length=224, Percent_Identity=40.625, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI166795305, Length=154, Percent_Identity=38.961038961039, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=48.8165680473373, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1787462, Length=288, Percent_Identity=40.2777777777778, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=305, Percent_Identity=30.4918032786885, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=255, Percent_Identity=38.4313725490196, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19921226, Length=237, Percent_Identity=42.6160337552743, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 43021; Mature: 43021

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSLKELKKQTTEMIESFQTYWTAQNFQEDYDRLLSLIEKANDPKLWDSPDQAKNVTQK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RNELQMKLDPWLGLKKELLDFPDLIELTSEEMGESGLKSLNDDFDRMFESFENLQMLDAL
HHHHEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKDDGKAAFINIHPGAGGTESQDWADMLLRMYTRFAEQKGYRAELVDYQPGETAGIKNA
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCE
TLYIQGDHPFGYLKCESGVHRLVRISPFDSNKRRHTSFASVYVTPEVDDDIQVNIEEKDL
EEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCE
RVDVYRSSGAGGQHVNTTDSAVRITHIPTGVVVSCQMERSQIKNRDTAMKMLKARLYEME
EEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHH
KQKAEEENAKKAGEKRDIAWGSQIRSYVFHPYNLVKDHRTDFETGNVHAVMDGDLEDFII
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCHHHHHH
AYLKYLTNQKANAKV
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MDRSLKELKKQTTEMIESFQTYWTAQNFQEDYDRLLSLIEKANDPKLWDSPDQAKNVTQK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RNELQMKLDPWLGLKKELLDFPDLIELTSEEMGESGLKSLNDDFDRMFESFENLQMLDAL
HHHHEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKDDGKAAFINIHPGAGGTESQDWADMLLRMYTRFAEQKGYRAELVDYQPGETAGIKNA
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCE
TLYIQGDHPFGYLKCESGVHRLVRISPFDSNKRRHTSFASVYVTPEVDDDIQVNIEEKDL
EEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCE
RVDVYRSSGAGGQHVNTTDSAVRITHIPTGVVVSCQMERSQIKNRDTAMKMLKARLYEME
EEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHH
KQKAEEENAKKAGEKRDIAWGSQIRSYVFHPYNLVKDHRTDFETGNVHAVMDGDLEDFII
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCHHHHHH
AYLKYLTNQKANAKV
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA