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The map label for this gene is nifS [H]

Identifier: 18311629

GI number: 18311629

Start: 3018663

End: 3019823

Strand: Direct

Name: nifS [H]

Synonym: CPE2647

Alternate gene names: 18311629

Gene position: 3018663-3019823 (Clockwise)

Preceding gene: 18311628

Following gene: 18311630

Centisome position: 99.58

GC content: 30.15

Gene sequence:

>1161_bases
ATGAATAAAATATATTTTGATAATGCAGCAACTACTTTCCCTAAACCTGACTCTGTAATAAAAGCAATGTTTGATTACAT
GAGTTTTGAAGGTGGAAGTGCTAATAGAGGATCCTCATCTACAGCTCTACAAAGTAGTAGAGCTGTCTATGAATGTAGAT
ATGAAATAGCTAAATTTTTTAATTTTCCTAAAAGTGAAAATGTTATTTTCACCAATAATATTACAACATCATTAAATATG
TTACTTTTAGGAATAATTAAATCTGATTGGCATATAATTACTACATCTATGGAACATAATTCTGTCTTAAGACCTTTAGT
AAAAATTAGCGAGGAGCTTCCTAATGTAGAACTAGATATAGTTCAATGTAATAATGAAGGTCTAGTATCAGTTGAAAAAA
TAAAAGAGAAATTAAAGAGTAACACAAAGCTTATAATTTTATCTCATGCATCAAACCTAGTTGGAACAATTCAACCAATT
AAAGAAATAGGGAAGCTTTGCAAAGAAAATAATATCTTTTTTATTTTAGATTCTGCTCAAACAGCAGGGGTTATTCCAAT
TGATATGACTGAACTTAATTTAAATGCATTAGCCTTTACAGGTCATAAGGCTCTTTTGGGTCCTCAAGGAATAGGTGGTT
TTATTATAGATGATAAATTAAATTCTATATGTAAAAATATCTTTTCTGGCGGAACAGGAAGTAATTCCTCACTAATAGAA
CATCCTCAAGAATTGCCTGATAAATTTGAATATGGAACTTTAAACACTCCAGGAATAATAGGGCTTCTAGAGGGAATAAA
ATTCATAGAAAAAGAAGGCATTGAAAATATAAAAGCAAAAGAAGAAGTATTATGCCAAAAAGCTATGGATTTATTATCTG
AAATTCCAGAAGTTAAGATTTATGGTCCTATGGATGCCAAAAAGAAAACTTCAACAATATCTTTCAATATAGAAGGTATG
GATCCTGAATTTACAGGATTCTTGTTAGATAGTGAATTTAACATAACATGTAGGACTGGAATTCATTGTACTCCGCTTGC
TCATAAAACAGTTGGTTCATACCCAGCTGGAAGCATAAGAATAAGTTTAGGGTACTTTAATACAATAGAAGAAGTCTATA
GATTTGTTGAGGTTATAAAAGAATTAATTTCAAGGAGGTAG

Upstream 100 bases:

>100_bases
CTGGTAAGGCTGATAAAAGTGTAAAATATTTTATGATTTTACTTGGATTAGGATTTACTATATACGGAATATTTAAAATT
TTTATGTGAGGTTTTTGCTT

Downstream 100 bases:

>100_bases
ACTTCTGACCTATGAAAAATTTAATAATCAAAAGTGAAAAAATAAGTGTGTTTTTTATTTTATACACACTTATATTTTTT
ACGTTTTTTAATACATTAAA

Product: cysteine desulfurase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MNKIYFDNAATTFPKPDSVIKAMFDYMSFEGGSANRGSSSTALQSSRAVYECRYEIAKFFNFPKSENVIFTNNITTSLNM
LLLGIIKSDWHIITTSMEHNSVLRPLVKISEELPNVELDIVQCNNEGLVSVEKIKEKLKSNTKLIILSHASNLVGTIQPI
KEIGKLCKENNIFFILDSAQTAGVIPIDMTELNLNALAFTGHKALLGPQGIGGFIIDDKLNSICKNIFSGGTGSNSSLIE
HPQELPDKFEYGTLNTPGIIGLLEGIKFIEKEGIENIKAKEEVLCQKAMDLLSEIPEVKIYGPMDAKKKTSTISFNIEGM
DPEFTGFLLDSEFNITCRTGIHCTPLAHKTVGSYPAGSIRISLGYFNTIEEVYRFVEVIKELISRR

Sequences:

>Translated_386_residues
MNKIYFDNAATTFPKPDSVIKAMFDYMSFEGGSANRGSSSTALQSSRAVYECRYEIAKFFNFPKSENVIFTNNITTSLNM
LLLGIIKSDWHIITTSMEHNSVLRPLVKISEELPNVELDIVQCNNEGLVSVEKIKEKLKSNTKLIILSHASNLVGTIQPI
KEIGKLCKENNIFFILDSAQTAGVIPIDMTELNLNALAFTGHKALLGPQGIGGFIIDDKLNSICKNIFSGGTGSNSSLIE
HPQELPDKFEYGTLNTPGIIGLLEGIKFIEKEGIENIKAKEEVLCQKAMDLLSEIPEVKIYGPMDAKKKTSTISFNIEGM
DPEFTGFLLDSEFNITCRTGIHCTPLAHKTVGSYPAGSIRISLGYFNTIEEVYRFVEVIKELISRR
>Mature_386_residues
MNKIYFDNAATTFPKPDSVIKAMFDYMSFEGGSANRGSSSTALQSSRAVYECRYEIAKFFNFPKSENVIFTNNITTSLNM
LLLGIIKSDWHIITTSMEHNSVLRPLVKISEELPNVELDIVQCNNEGLVSVEKIKEKLKSNTKLIILSHASNLVGTIQPI
KEIGKLCKENNIFFILDSAQTAGVIPIDMTELNLNALAFTGHKALLGPQGIGGFIIDDKLNSICKNIFSGGTGSNSSLIE
HPQELPDKFEYGTLNTPGIIGLLEGIKFIEKEGIENIKAKEEVLCQKAMDLLSEIPEVKIYGPMDAKKKTSTISFNIEGM
DPEFTGFLLDSEFNITCRTGIHCTPLAHKTVGSYPAGSIRISLGYFNTIEEVYRFVEVIKELISRR

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=24.25, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI156713448, Length=444, Percent_Identity=23.6486486486486, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787970, Length=387, Percent_Identity=29.1989664082687, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI48994898, Length=399, Percent_Identity=26.5664160401003, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789175, Length=387, Percent_Identity=25.3229974160207, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25143064, Length=394, Percent_Identity=27.9187817258883, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17533177, Length=346, Percent_Identity=30.0578034682081, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193211090, Length=345, Percent_Identity=27.8260869565217, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25147498, Length=441, Percent_Identity=24.7165532879819, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319831, Length=395, Percent_Identity=29.3670886075949, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI20129463, Length=404, Percent_Identity=27.4752475247525, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42753; Mature: 42753

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIYFDNAATTFPKPDSVIKAMFDYMSFEGGSANRGSSSTALQSSRAVYECRYEIAKFF
CCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NFPKSENVIFTNNITTSLNMLLLGIIKSDWHIITTSMEHNSVLRPLVKISEELPNVELDI
CCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEE
VQCNNEGLVSVEKIKEKLKSNTKLIILSHASNLVGTIQPIKEIGKLCKENNIFFILDSAQ
EEECCCCCEEHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TAGVIPIDMTELNLNALAFTGHKALLGPQGIGGFIIDDKLNSICKNIFSGGTGSNSSLIE
CCCEEEEEEEEECCEEEEEECCCEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHH
HPQELPDKFEYGTLNTPGIIGLLEGIKFIEKEGIENIKAKEEVLCQKAMDLLSEIPEVKI
CHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEE
YGPMDAKKKTSTISFNIEGMDPEFTGFLLDSEFNITCRTGIHCTPLAHKTVGSYPAGSIR
ECCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEECCCEECCCHHHHCCCCCCCEEE
ISLGYFNTIEEVYRFVEVIKELISRR
EEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKIYFDNAATTFPKPDSVIKAMFDYMSFEGGSANRGSSSTALQSSRAVYECRYEIAKFF
CCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NFPKSENVIFTNNITTSLNMLLLGIIKSDWHIITTSMEHNSVLRPLVKISEELPNVELDI
CCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEE
VQCNNEGLVSVEKIKEKLKSNTKLIILSHASNLVGTIQPIKEIGKLCKENNIFFILDSAQ
EEECCCCCEEHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TAGVIPIDMTELNLNALAFTGHKALLGPQGIGGFIIDDKLNSICKNIFSGGTGSNSSLIE
CCCEEEEEEEEECCEEEEEECCCEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHH
HPQELPDKFEYGTLNTPGIIGLLEGIKFIEKEGIENIKAKEEVLCQKAMDLLSEIPEVKI
CHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEE
YGPMDAKKKTSTISFNIEGMDPEFTGFLLDSEFNITCRTGIHCTPLAHKTVGSYPAGSIR
ECCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEECCCEECCCHHHHCCCCCCCEEE
ISLGYFNTIEEVYRFVEVIKELISRR
EEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]