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The map label for this gene is proB

Identifier: 18311561

GI number: 18311561

Start: 2954670

End: 2955479

Strand: Reverse

Name: proB

Synonym: CPE2579

Alternate gene names: 18311561

Gene position: 2955479-2954670 (Counterclockwise)

Preceding gene: 18311562

Following gene: 18311560

Centisome position: 97.49

GC content: 30.62

Gene sequence:

>810_bases
ATGAGAAAAGCATTAAAAGATAGTAAAAGAATAGTTGTTAAGGTTGGAACTTCTACATTAACTTATGAAAATGGAAGTGT
AAACTTAATGAGAATAGAAAAATTATCTAGAGTAATTTCAGATATATGTAATATGGGAAAAGAAGTTGTTCTTGTTTCAT
CAGGAGCAATTGGAGTTGGAGTTGGAAAACTTAGACTTGAGAAAAAACCAGAGACTATAAGAGAAAAGCAAGCTGTAGCT
GCCGTTGGACAGTGCGCTTTAATGCATATATATAATAAATTTTTTGGAGAATATAGTCATGTTGTGGCACAGGTTCTTTT
AACTAGAGATGTACTAGAAAATGAAAGAATGAAAAATAACGTATGTAATACATTTGATACTTTATTAGAAAAAGGAATTA
TACCTATAGTTAATGAGAATGATGCTATATCAATAGATGAGATACAAAACATATTAAACTTTGGAGATAATGATAATCTT
TCAGCTATAGTTTCTACTTTAGTAAATGCAGATACTTTAATAATATTATCTGATATAGATGGTTTCTACGATTCAGATCC
AAGAACTAATGAGGATAGTAAGATGCTTAAAGAAGTTTATGAAATAACTCCTGAAATAGAAGAATGTGCAGGGGGAGCTG
GAAGTAATAGAGGAACAGGCGGAATGGTAACTAAACTTTCAGCAGCTAAGGTAGCAACAAGTAATGGAATAGATATGATT
TTAGCTAATGGAGAAAATCCAGAAATATTAATTGACATATTAAATGGTGAAGATATAGGAACTATGTTTGTAGCTAAGAA
GGGGGAATAA

Upstream 100 bases:

>100_bases
TTATAAATGGGTAGTTTCATGATTTAACGTATTAGAATATAATATACTTATGATTTAATGGAAAAATAATTTTTATAAAT
AAAAAGGGGGAAACCTACAA

Downstream 100 bases:

>100_bases
AAATGAATAATGAACTTATTATAAAAGGTAAAAAAGCAAAAGAAGCTTCTTACACACTTTCATTTGCATCTACTAATGAA
AAAGATAATGGATTATTAAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRKALKDSKRIVVKVGTSTLTYENGSVNLMRIEKLSRVISDICNMGKEVVLVSSGAIGVGVGKLRLEKKPETIREKQAVA
AVGQCALMHIYNKFFGEYSHVVAQVLLTRDVLENERMKNNVCNTFDTLLEKGIIPIVNENDAISIDEIQNILNFGDNDNL
SAIVSTLVNADTLIILSDIDGFYDSDPRTNEDSKMLKEVYEITPEIEECAGGAGSNRGTGGMVTKLSAAKVATSNGIDMI
LANGENPEILIDILNGEDIGTMFVAKKGE

Sequences:

>Translated_269_residues
MRKALKDSKRIVVKVGTSTLTYENGSVNLMRIEKLSRVISDICNMGKEVVLVSSGAIGVGVGKLRLEKKPETIREKQAVA
AVGQCALMHIYNKFFGEYSHVVAQVLLTRDVLENERMKNNVCNTFDTLLEKGIIPIVNENDAISIDEIQNILNFGDNDNL
SAIVSTLVNADTLIILSDIDGFYDSDPRTNEDSKMLKEVYEITPEIEECAGGAGSNRGTGGMVTKLSAAKVATSNGIDMI
LANGENPEILIDILNGEDIGTMFVAKKGE
>Mature_269_residues
MRKALKDSKRIVVKVGTSTLTYENGSVNLMRIEKLSRVISDICNMGKEVVLVSSGAIGVGVGKLRLEKKPETIREKQAVA
AVGQCALMHIYNKFFGEYSHVVAQVLLTRDVLENERMKNNVCNTFDTLLEKGIIPIVNENDAISIDEIQNILNFGDNDNL
SAIVSTLVNADTLIILSDIDGFYDSDPRTNEDSKMLKEVYEITPEIEECAGGAGSNRGTGGMVTKLSAAKVATSNGIDMI
LANGENPEILIDILNGEDIGTMFVAKKGE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=291, Percent_Identity=32.6460481099656, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI21361368, Length=293, Percent_Identity=32.7645051194539, Blast_Score=147, Evalue=7e-36,
Organism=Escherichia coli, GI1786437, Length=262, Percent_Identity=41.2213740458015, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17569967, Length=281, Percent_Identity=36.6548042704626, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17569969, Length=281, Percent_Identity=36.6548042704626, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321822, Length=249, Percent_Identity=42.1686746987952, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320506, Length=244, Percent_Identity=41.3934426229508, Blast_Score=177, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=34.4827586206897, Blast_Score=147, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_CLOPE (Q8XHA6)

Other databases:

- EMBL:   AJ420783
- EMBL:   BA000016
- RefSeq:   NP_563495.1
- ProteinModelPortal:   Q8XHA6
- SMR:   Q8XHA6
- GeneID:   990956
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE2579
- NMPDR:   fig|195102.1.peg.2642
- HOGENOM:   HBG507643
- OMA:   LINTIDP
- PhylomeDB:   Q8XHA6
- ProtClustDB:   PRK12314
- BioCyc:   CPER195102:CPE2579-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.11

Molecular weight: Translated: 29215; Mature: 29215

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS00153 ATPASE_GAMMA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKALKDSKRIVVKVGTSTLTYENGSVNLMRIEKLSRVISDICNMGKEVVLVSSGAIGVG
CCCCCCCCCEEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCEEC
VGKLRLEKKPETIREKQAVAAVGQCALMHIYNKFFGEYSHVVAQVLLTRDVLENERMKNN
EEHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCNTFDTLLEKGIIPIVNENDAISIDEIQNILNFGDNDNLSAIVSTLVNADTLIILSDID
HHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCC
GFYDSDPRTNEDSKMLKEVYEITPEIEECAGGAGSNRGTGGMVTKLSAAKVATSNGIDMI
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEEHHHHHCCCCCCEEE
LANGENPEILIDILNGEDIGTMFVAKKGE
EECCCCCEEEEEEECCCCCEEEEEEECCC
>Mature Secondary Structure
MRKALKDSKRIVVKVGTSTLTYENGSVNLMRIEKLSRVISDICNMGKEVVLVSSGAIGVG
CCCCCCCCCEEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCEEC
VGKLRLEKKPETIREKQAVAAVGQCALMHIYNKFFGEYSHVVAQVLLTRDVLENERMKNN
EEHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCNTFDTLLEKGIIPIVNENDAISIDEIQNILNFGDNDNLSAIVSTLVNADTLIILSDID
HHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCC
GFYDSDPRTNEDSKMLKEVYEITPEIEECAGGAGSNRGTGGMVTKLSAAKVATSNGIDMI
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEEHHHHHCCCCCCEEE
LANGENPEILIDILNGEDIGTMFVAKKGE
EECCCCCEEEEEEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842