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The map label for this gene is glpF [H]

Identifier: 18311536

GI number: 18311536

Start: 2924929

End: 2925636

Strand: Reverse

Name: glpF [H]

Synonym: CPE2554

Alternate gene names: 18311536

Gene position: 2925636-2924929 (Counterclockwise)

Preceding gene: 18311537

Following gene: 18311535

Centisome position: 96.51

GC content: 35.31

Gene sequence:

>708_bases
ATGGTAAATCAATTCTTGGCTGAGCTTTTTGGAACTGCGCTTTTGATATTGTTAGGGGATGGAGTAGTTGCAAATATAGC
TCTTAAAAAAACTAAGGGTAATGGCGCTGATCTTATTGTAGTCACTGTAGGATGGGCAATAGCTGTTGCTATTCCTGTTT
ATATCTTTTCTAGGGTGAGTGGAGCTGTATTTAATCCAGCGGTAACTATTGCATTAGGTATAACTGGAGGATTAACATGG
AGTCAGGTTATAGTATTTTGTATAGCACAATTTATAGGAGCTTTCATAGGTGCTGTACTAGTTTATTTAACTTATTATAC
TCATTTTGAGTCTACTGAAGATCATGATGCAAAGCTTGGTACATTTTGTACTATACCAGCTATTAGAAATTTTAAATGGA
ATTTTTTTACTGAATTTATAGGTACTTTTGTTTTAATGTTTGGAATTCTAGGACTTGGTGATGCGAAGATGGCAGATGGA
ATTATGCCAATAGCCGTTGGATTATTAATTTTAGTTATAGGAGTATCTTTAGGCGGACCAACTGGATATGCAATTAATCC
AGCTAGAGATTTCTCACCTAGACTTGCACATTTCTTGCTTCCTATGAAAAATAAAGGAGGCTCAGATTGGGCTTATGCTT
GGATTCCAATTGTAGGACCAATACTTGGAGCTATAGCAGGAGCAATGGTATATACAAATATATTTTAA

Upstream 100 bases:

>100_bases
AGTTAAACACGAGAAAAAAATTTAGTTTTTTTTGTTTAGCTCTTTTTTATTTTAAGCTTTTTTAAAATTAAATATATTAA
TTACTAGGTGGTGGATAGTC

Downstream 100 bases:

>100_bases
GGAGATATTAATTTTGGACAATTTTAAGGAGGCTATATAAAATGATTAATTTTAAAAGAATACTTGAGGACAACCCTATT
GTGGCAGCTGTAAAGGATAT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MVNQFLAELFGTALLILLGDGVVANIALKKTKGNGADLIVVTVGWAIAVAIPVYIFSRVSGAVFNPAVTIALGITGGLTW
SQVIVFCIAQFIGAFIGAVLVYLTYYTHFESTEDHDAKLGTFCTIPAIRNFKWNFFTEFIGTFVLMFGILGLGDAKMADG
IMPIAVGLLILVIGVSLGGPTGYAINPARDFSPRLAHFLLPMKNKGGSDWAYAWIPIVGPILGAIAGAMVYTNIF

Sequences:

>Translated_235_residues
MVNQFLAELFGTALLILLGDGVVANIALKKTKGNGADLIVVTVGWAIAVAIPVYIFSRVSGAVFNPAVTIALGITGGLTW
SQVIVFCIAQFIGAFIGAVLVYLTYYTHFESTEDHDAKLGTFCTIPAIRNFKWNFFTEFIGTFVLMFGILGLGDAKMADG
IMPIAVGLLILVIGVSLGGPTGYAINPARDFSPRLAHFLLPMKNKGGSDWAYAWIPIVGPILGAIAGAMVYTNIF
>Mature_235_residues
MVNQFLAELFGTALLILLGDGVVANIALKKTKGNGADLIVVTVGWAIAVAIPVYIFSRVSGAVFNPAVTIALGITGGLTW
SQVIVFCIAQFIGAFIGAVLVYLTYYTHFESTEDHDAKLGTFCTIPAIRNFKWNFFTEFIGTFVLMFGILGLGDAKMADG
IMPIAVGLLILVIGVSLGGPTGYAINPARDFSPRLAHFLLPMKNKGGSDWAYAWIPIVGPILGAIAGAMVYTNIF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=246, Percent_Identity=35.7723577235772, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4826645, Length=246, Percent_Identity=36.5853658536585, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI4502187, Length=245, Percent_Identity=33.469387755102, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=31.3008130081301, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI310133356, Length=185, Percent_Identity=30.8108108108108, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI310114181, Length=185, Percent_Identity=30.8108108108108, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790362, Length=253, Percent_Identity=40.7114624505929, Blast_Score=130, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71994009, Length=242, Percent_Identity=35.1239669421488, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17544068, Length=251, Percent_Identity=33.4661354581673, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531429, Length=241, Percent_Identity=34.8547717842324, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17531431, Length=241, Percent_Identity=34.8547717842324, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71992966, Length=247, Percent_Identity=31.9838056680162, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32564052, Length=242, Percent_Identity=29.3388429752066, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533613, Length=242, Percent_Identity=29.3388429752066, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71992961, Length=245, Percent_Identity=29.7959183673469, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558372, Length=185, Percent_Identity=28.1081081081081, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=203, Percent_Identity=31.5270935960591, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322985, Length=277, Percent_Identity=29.6028880866426, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45550503, Length=243, Percent_Identity=28.3950617283951, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24998; Mature: 24998

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNQFLAELFGTALLILLGDGVVANIALKKTKGNGADLIVVTVGWAIAVAIPVYIFSRVS
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHC
GAVFNPAVTIALGITGGLTWSQVIVFCIAQFIGAFIGAVLVYLTYYTHFESTEDHDAKLG
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TFCTIPAIRNFKWNFFTEFIGTFVLMFGILGLGDAKMADGIMPIAVGLLILVIGVSLGGP
CEEECCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCCC
TGYAINPARDFSPRLAHFLLPMKNKGGSDWAYAWIPIVGPILGAIAGAMVYTNIF
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVNQFLAELFGTALLILLGDGVVANIALKKTKGNGADLIVVTVGWAIAVAIPVYIFSRVS
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHC
GAVFNPAVTIALGITGGLTWSQVIVFCIAQFIGAFIGAVLVYLTYYTHFESTEDHDAKLG
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TFCTIPAIRNFKWNFFTEFIGTFVLMFGILGLGDAKMADGIMPIAVGLLILVIGVSLGGP
CEEECCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCCC
TGYAINPARDFSPRLAHFLLPMKNKGGSDWAYAWIPIVGPILGAIAGAMVYTNIF
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]