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The map label for this gene is ksgA

Identifier: 18311499

GI number: 18311499

Start: 2881782

End: 2882639

Strand: Reverse

Name: ksgA

Synonym: CPE2517

Alternate gene names: 18311499

Gene position: 2882639-2881782 (Counterclockwise)

Preceding gene: 18311500

Following gene: 18311498

Centisome position: 95.09

GC content: 29.25

Gene sequence:

>858_bases
ATGGATATAAACGAAATAAAAGATATAAAGACAAAGGAGCTTGTTCAGAAGTATAATTTTAGATTTTCAAAAAGCTTAGG
ACAAAACTTTTTAATTGATGATTCTGTTCCAAGAGATATAGTAAATGGAGCAGATGTTTGTGAAGATGATTTAGTAATAG
AAATTGGACCTGGAGTTGGTACTCTTACTGTACAATTACTTAAAAGAGCAAAGAGAGTTGTTGCTATTGAATTAGATAGT
TCGCTTATTCCAATATTAACAGCTGAGCTTGGAGATAATCCAAAGTTTCAATTAATACATAATGATGCCTTAAAAGTTGA
TTTTAATGAAATTATAGGAGATGAAAAAAGTGTTAAGCTAGTTGCAAACTTACCTTATTATGTAACTACTCCTATAATAG
TAAATTTATTAAAAGGTGGATATAACTTTAAGTCATTAACTATAATGATACAAAAAGAAGTAGCAGAAAGAATGAATGCA
GAACCTAATTGTAAAGATTATGGTGCTTTATCAATTTTAGTTCAATACTACTGTAATACTAAAATAGTTAGAAAGGTACC
ACCTTCATGCTTCATACCAAGACCAAAGGTTGATTCTATAGTAATAAGATTAGAAAGATTAGAAGAGCCAAGTGTTAAAG
TTAAAAATGAAAAATTATTCTTTGAAATTGTTAGACATGCATTTAACATGAGAAGAAAAACTTTATGGAATGCAACTAAA
AATGTAAAACTTCCTAAGGAATTAATGGAAAAGGCTTATGAAGAGGCTGGAATAGATCCTAAGAGAAGAGGAGAGACTTT
AAGCTTAGCAGAGTTTGGTGCTCTTTCAGATGCAATAGATAAATATATGAATAACTAG

Upstream 100 bases:

>100_bases
CAAACCAAGTTCTTTCAAGATTAAATAACTATGGCATAACAAAAGAAGAATTTGTTAAAGCTATAGAAAAGATAGAAAAG
ATGATATAGGAGAGTAGTGA

Downstream 100 bases:

>100_bases
AATATTTATAGAGAATAAGAATTTTTAAATTTTTATTCTCTTTTTTTTTGTTCTCTCATATACTGTAGAGAATAAAATTA
TATTGGAGGGACCAGAGAAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MDINEIKDIKTKELVQKYNFRFSKSLGQNFLIDDSVPRDIVNGADVCEDDLVIEIGPGVGTLTVQLLKRAKRVVAIELDS
SLIPILTAELGDNPKFQLIHNDALKVDFNEIIGDEKSVKLVANLPYYVTTPIIVNLLKGGYNFKSLTIMIQKEVAERMNA
EPNCKDYGALSILVQYYCNTKIVRKVPPSCFIPRPKVDSIVIRLERLEEPSVKVKNEKLFFEIVRHAFNMRRKTLWNATK
NVKLPKELMEKAYEEAGIDPKRRGETLSLAEFGALSDAIDKYMNN

Sequences:

>Translated_285_residues
MDINEIKDIKTKELVQKYNFRFSKSLGQNFLIDDSVPRDIVNGADVCEDDLVIEIGPGVGTLTVQLLKRAKRVVAIELDS
SLIPILTAELGDNPKFQLIHNDALKVDFNEIIGDEKSVKLVANLPYYVTTPIIVNLLKGGYNFKSLTIMIQKEVAERMNA
EPNCKDYGALSILVQYYCNTKIVRKVPPSCFIPRPKVDSIVIRLERLEEPSVKVKNEKLFFEIVRHAFNMRRKTLWNATK
NVKLPKELMEKAYEEAGIDPKRRGETLSLAEFGALSDAIDKYMNN
>Mature_285_residues
MDINEIKDIKTKELVQKYNFRFSKSLGQNFLIDDSVPRDIVNGADVCEDDLVIEIGPGVGTLTVQLLKRAKRVVAIELDS
SLIPILTAELGDNPKFQLIHNDALKVDFNEIIGDEKSVKLVANLPYYVTTPIIVNLLKGGYNFKSLTIMIQKEVAERMNA
EPNCKDYGALSILVQYYCNTKIVRKVPPSCFIPRPKVDSIVIRLERLEEPSVKVKNEKLFFEIVRHAFNMRRKTLWNATK
NVKLPKELMEKAYEEAGIDPKRRGETLSLAEFGALSDAIDKYMNN

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=292, Percent_Identity=30.4794520547945, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI7657198, Length=236, Percent_Identity=34.7457627118644, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1786236, Length=255, Percent_Identity=35.6862745098039, Blast_Score=158, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI25141369, Length=298, Percent_Identity=30.2013422818792, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI25146882, Length=188, Percent_Identity=35.1063829787234, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=241, Percent_Identity=31.1203319502075, Blast_Score=105, Evalue=8e-24,
Organism=Drosophila melanogaster, GI21358017, Length=245, Percent_Identity=35.5102040816327, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21357273, Length=290, Percent_Identity=27.9310344827586, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_CLOP1 (Q0TMD6)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_697204.1
- ProteinModelPortal:   Q0TMD6
- SMR:   Q0TMD6
- STRING:   Q0TMD6
- GeneID:   4202275
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2840
- TIGR:   CPF_2840
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   CPER195103:CPF_2840-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32363; Mature: 32363

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 29-29 BINDING 31-31 BINDING 56-56 BINDING 77-77 BINDING 102-102 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDINEIKDIKTKELVQKYNFRFSKSLGQNFLIDDSVPRDIVNGADVCEDDLVIEIGPGVG
CCCHHHHCCHHHHHHHHHCCHHHHHCCCCEEECCCCCHHHHCCCCCCCCCEEEEECCCCH
TLTVQLLKRAKRVVAIELDSSLIPILTAELGDNPKFQLIHNDALKVDFNEIIGDEKSVKL
HHHHHHHHHHHHEEEEEECCCCCCEEECCCCCCCCEEEEECCCEEEEHHHHHCCCCCEEE
VANLPYYVTTPIIVNLLKGGYNFKSLTIMIQKEVAERMNAEPNCKDYGALSILVQYYCNT
EECCCCHHHHHHHHHHHHCCCCEEHEEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
KIVRKVPPSCFIPRPKVDSIVIRLERLEEPSVKVKNEKLFFEIVRHAFNMRRKTLWNATK
EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCC
NVKLPKELMEKAYEEAGIDPKRRGETLSLAEFGALSDAIDKYMNN
CCCCCHHHHHHHHHHHCCCHHHCCCEECHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDINEIKDIKTKELVQKYNFRFSKSLGQNFLIDDSVPRDIVNGADVCEDDLVIEIGPGVG
CCCHHHHCCHHHHHHHHHCCHHHHHCCCCEEECCCCCHHHHCCCCCCCCCEEEEECCCCH
TLTVQLLKRAKRVVAIELDSSLIPILTAELGDNPKFQLIHNDALKVDFNEIIGDEKSVKL
HHHHHHHHHHHHEEEEEECCCCCCEEECCCCCCCCEEEEECCCEEEEHHHHHCCCCCEEE
VANLPYYVTTPIIVNLLKGGYNFKSLTIMIQKEVAERMNAEPNCKDYGALSILVQYYCNT
EECCCCHHHHHHHHHHHHCCCCEEHEEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
KIVRKVPPSCFIPRPKVDSIVIRLERLEEPSVKVKNEKLFFEIVRHAFNMRRKTLWNATK
EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCC
NVKLPKELMEKAYEEAGIDPKRRGETLSLAEFGALSDAIDKYMNN
CCCCCHHHHHHHHHHHCCCHHHCCCEECHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA