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The map label for this gene is dus1 [H]

Identifier: 18311449

GI number: 18311449

Start: 2823152

End: 2824132

Strand: Reverse

Name: dus1 [H]

Synonym: CPE2467

Alternate gene names: 18311449

Gene position: 2824132-2823152 (Counterclockwise)

Preceding gene: 18311450

Following gene: 18311448

Centisome position: 93.16

GC content: 32.93

Gene sequence:

>981_bases
ATGAAAATATCAAACATAGTTTTAGAAAATAATGTTTTCTTAGCACCTATGGCAGGGGTTACAGATATATCTTTTAGGGG
ATTATGTAAAGAGATGGGGGCAGGAATTGTTTGCACTGAAATGGTTAGTGCAAAGGCATTGTACTATGGGAGTGAAAATA
CAAAAGAATTAATGAGAATTGCGGATGAAGAAAAACCGGTATCTTGTCAGATGTTTGGAAATGATCCAGAGATAATGGCT
TATGTCACTGAGAAGCATTTTAATACAAATGATGATATATGTATAATAGATGTGAATATGGGATGTCCTGCACCTAAGAT
AGTAAAAAATGGTGAAGGTTCAGCCTTAATGAAAAATCCTAAATTAGCTTATGAGATCATGAGTAGCATAAAAAAAGTTG
CAACAAAGCCTGTAACAGTTAAGTTTAGAATGGGTTTTGATGAAGAAAATATAAATGCTGTTGAGTTTGCTAAAATCATG
GAAGAAGCAGGAGTAGATGCCATTGCTGTACATGGAAGAACTAGGGCTCAAATGTATGAAGGAAAAGCTAACTGGGATAT
AATAAGACAAGTAAAGGAAGCAGTTAATATTCCTGTTATAGGAAATGGAGATGTATTTACTGCGGAGGATGCTTTAAGTC
TTAAAAATCAAACTAACTGTGATGGAATTATGGTTGCAAGAGGAAGTATGGGAAACCCATGGATCTTCAAGCAAATAGTT
CAGAAGTTAAATGGAGAAGAGGTTTATATGCCTACTCCAGAAGAAAAAATAGATATGTGTATAAGACACTATAATTTATC
ACTTAAATATGATGGAGAGTATAAGGCTGTAAGAGAAATGAGAAAACATGCAGCTTGGTATATAAAAGGATTAAATAATT
GTACTGAAGTTAAGAATAGGCTTAATTTAGAAGCAGATCCAGAGAAGATTATGGGTATCCTTAATGAGTATAAAGAATAT
TTAATGAAAAATGATTTATAA

Upstream 100 bases:

>100_bases
ACTTGTTTATAGCGAAACGGATGTAATTGATGAAGTAGATCGTAAATTAACTTTAGAAGGGCTTAAGATTCTTTACGAAA
AAAACAAGGAGTAGAAAGTA

Downstream 100 bases:

>100_bases
TATTTATCATAAATGGGGATATAACTGGATATAATAAAGCATCAGTAAAATATTAGATGTTTAATTTATACAAATAAAGG
TGAGAATTAAAGCTAAATAA

Product: putative dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKISNIVLENNVFLAPMAGVTDISFRGLCKEMGAGIVCTEMVSAKALYYGSENTKELMRIADEEKPVSCQMFGNDPEIMA
YVTEKHFNTNDDICIIDVNMGCPAPKIVKNGEGSALMKNPKLAYEIMSSIKKVATKPVTVKFRMGFDEENINAVEFAKIM
EEAGVDAIAVHGRTRAQMYEGKANWDIIRQVKEAVNIPVIGNGDVFTAEDALSLKNQTNCDGIMVARGSMGNPWIFKQIV
QKLNGEEVYMPTPEEKIDMCIRHYNLSLKYDGEYKAVREMRKHAAWYIKGLNNCTEVKNRLNLEADPEKIMGILNEYKEY
LMKNDL

Sequences:

>Translated_326_residues
MKISNIVLENNVFLAPMAGVTDISFRGLCKEMGAGIVCTEMVSAKALYYGSENTKELMRIADEEKPVSCQMFGNDPEIMA
YVTEKHFNTNDDICIIDVNMGCPAPKIVKNGEGSALMKNPKLAYEIMSSIKKVATKPVTVKFRMGFDEENINAVEFAKIM
EEAGVDAIAVHGRTRAQMYEGKANWDIIRQVKEAVNIPVIGNGDVFTAEDALSLKNQTNCDGIMVARGSMGNPWIFKQIV
QKLNGEEVYMPTPEEKIDMCIRHYNLSLKYDGEYKAVREMRKHAAWYIKGLNNCTEVKNRLNLEADPEKIMGILNEYKEY
LMKNDL
>Mature_326_residues
MKISNIVLENNVFLAPMAGVTDISFRGLCKEMGAGIVCTEMVSAKALYYGSENTKELMRIADEEKPVSCQMFGNDPEIMA
YVTEKHFNTNDDICIIDVNMGCPAPKIVKNGEGSALMKNPKLAYEIMSSIKKVATKPVTVKFRMGFDEENINAVEFAKIM
EEAGVDAIAVHGRTRAQMYEGKANWDIIRQVKEAVNIPVIGNGDVFTAEDALSLKNQTNCDGIMVARGSMGNPWIFKQIV
QKLNGEEVYMPTPEEKIDMCIRHYNLSLKYDGEYKAVREMRKHAAWYIKGLNNCTEVKNRLNLEADPEKIMGILNEYKEY
LMKNDL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=324, Percent_Identity=29.0123456790123, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI239788462, Length=320, Percent_Identity=29.375, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=31.304347826087, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=30.2788844621514, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=27.4336283185841, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=36.4485981308411, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788462, Length=300, Percent_Identity=28.6666666666667, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI145693211, Length=264, Percent_Identity=25, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=241, Percent_Identity=32.7800829875519, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=30.0448430493274, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=31.9838056680162, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17507177, Length=228, Percent_Identity=28.9473684210526, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=284, Percent_Identity=28.5211267605634, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323560, Length=281, Percent_Identity=29.1814946619217, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323437, Length=225, Percent_Identity=28.4444444444444, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585320, Length=281, Percent_Identity=30.6049822064057, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24580595, Length=229, Percent_Identity=32.3144104803493, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19920448, Length=229, Percent_Identity=32.3144104803493, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19921524, Length=285, Percent_Identity=26.3157894736842, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45549423, Length=248, Percent_Identity=30.6451612903226, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36654; Mature: 36654

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISNIVLENNVFLAPMAGVTDISFRGLCKEMGAGIVCTEMVSAKALYYGSENTKELMRI
CCCCCEEEECCEEEEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHEEEECCCCHHHHHHH
ADEEKPVSCQMFGNDPEIMAYVTEKHFNTNDDICIIDVNMGCPAPKIVKNGEGSALMKNP
HCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCCCCCHHHEECCCCCEEECCC
KLAYEIMSSIKKVATKPVTVKFRMGFDEENINAVEFAKIMEEAGVDAIAVHGRTRAQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHCC
GKANWDIIRQVKEAVNIPVIGNGDVFTAEDALSLKNQTNCDGIMVARGSMGNPWIFKQIV
CCCCHHHHHHHHHHHCCCEECCCCEEECHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHH
QKLNGEEVYMPTPEEKIDMCIRHYNLSLKYDGEYKAVREMRKHAAWYIKGLNNCTEVKNR
HHCCCCEEEECCCHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
LNLEADPEKIMGILNEYKEYLMKNDL
CCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKISNIVLENNVFLAPMAGVTDISFRGLCKEMGAGIVCTEMVSAKALYYGSENTKELMRI
CCCCCEEEECCEEEEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHEEEECCCCHHHHHHH
ADEEKPVSCQMFGNDPEIMAYVTEKHFNTNDDICIIDVNMGCPAPKIVKNGEGSALMKNP
HCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCCCCCHHHEECCCCCEEECCC
KLAYEIMSSIKKVATKPVTVKFRMGFDEENINAVEFAKIMEEAGVDAIAVHGRTRAQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHCC
GKANWDIIRQVKEAVNIPVIGNGDVFTAEDALSLKNQTNCDGIMVARGSMGNPWIFKQIV
CCCCHHHHHHHHHHHCCCEECCCCEEECHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHH
QKLNGEEVYMPTPEEKIDMCIRHYNLSLKYDGEYKAVREMRKHAAWYIKGLNNCTEVKNR
HHCCCCEEEECCCHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
LNLEADPEKIMGILNEYKEYLMKNDL
CCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]