Click here to switch to the map view.

The map label for this gene is yaaO [H]

Identifier: 18311435

GI number: 18311435

Start: 2799143

End: 2800573

Strand: Reverse

Name: yaaO [H]

Synonym: CPE2453

Alternate gene names: 18311435

Gene position: 2800573-2799143 (Counterclockwise)

Preceding gene: 18311436

Following gene: 18311434

Centisome position: 92.38

GC content: 25.09

Gene sequence:

>1431_bases
TTGAGAAATATTCCTATAATTAATGGAATATCAATGTACTTAAGAGAAGAAATAGTACCTTTTTCTATGCCAGGTCATAA
GAATGGACGAGGCTTTAAGGGATTTGAAGAAATACTTTTAAAAGGTGATTTAACTGAAGTTGAAGGATTAGATAATTTGC
AGCATCCAGAGGGAATAATTAAAGAAGCTGAGGATAAACTTTCTAATTTATATAAAAGTAAAAAGAGTTATTTTTTAGTT
AATGGAAGTACTTGTGGAAATTTAATAATGATATTTTCTTTGTTTAAAGAAGGAGATAAAGTTTTAGTAGAAAGAAACTG
TCACAAAAGTATAATGAATGGAATAATAATGAGAAAATTAAAACCAGTTTTTATAAATAATATATTTCATTCAAAATTAA
AAGCACCAGTAGGAATTGATTTAAATCATTTAAAAGAGCAAATAAGTTTAAATAAAGATTTAAAAGGAATAATATTAACT
TACCCTAATTATTATGGCGTAGGAGTTAATATAGATGAGACTATAAAAATAGCTAGAGAAGCAAATTTAAAAGTAGGAAT
AGATTCAGCTCATGGAGCACACTTTGGATTTAACAAGTTATTGCCTGAAAGTGTACAAGAATTAAAGGCTGATATAGTTG
TAATGAGTGCTCATAAAACTTTACCAAGTTTAACACAGACTGCTTGGATGCATGTTAACAATGAAGATTTTATTGAAGAA
ATAGAATTTTATAAAAATATTTTTATGAGTACTAGCCCTTCATATATGTTTATGATGAGTTTAGATTATTCAAGAAACTT
TTTAGAAGAACATGGTGAAGAGGCATATAATAACTTATTTAAGATCATAGAGGATTTTAAAAATCATATTAAGGACTTAT
CATATTTTAAAATATTAGATAAGGAATTTTTAATTAATGGGTTAGAAAAAAATATTAAGATAAATGATATTAAGATAGAT
AATAGTAGAATAGTACTAAACTTAAGGGAAAATTTAAATGGTAATAAACTATTAGATTATTTAAGAAGTAAAAATATACA
ATGTGAAATGAGTGATAATAAAAATGTTGTTTTAATACCGTCACCATTTAATACTGAAGAAGATTTTAAAATTTTAAAAG
ATGCTTTAAAATCTTGTGATATAAATTGTCTTAAAGATGAAGAAATAGATTTTTATTTAACAGATTTACCAGAGAAAAAA
TTAGAACCTTTTGAAGTTTTGGATTTAGAATATGAAAGAATTAATATAAATGATGCTGTAGGTAAAATTGTGGCAGAGAG
TATAGTTCCATACCCACCAGGAGTTCCTATGATAATAATGGGAGAAGTGATAGAAGAAAGACATATTAAATTACTTAATG
AGTATATGAAAAGCAAGGTAGATTTAATAGGTATAAAGGATAAAAAAGTTAAGGTAATAAAGAATAAGTAA

Upstream 100 bases:

>100_bases
TAAGTTGTGATATTAATAGTGAATTCTATGAGTTTTATAAAAATAGATTAAAAGAAGAAGTTTATAAGAAAAAATTAGGA
TAACAGAGGAGTGGTTAACT

Downstream 100 bases:

>100_bases
ATACTTAAAGAATATATTAGTTAGGTTGTATCGAAGTTAGGAGTTAAATAAAGAATATAATAAGTATTTTTTATTTATAA
GATTTTGGTACAACCTTTTT

Product: Orn/Lys/Arg decarboxylase

Products: 1,5-Diaminopentane; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MRNIPIINGISMYLREEIVPFSMPGHKNGRGFKGFEEILLKGDLTEVEGLDNLQHPEGIIKEAEDKLSNLYKSKKSYFLV
NGSTCGNLIMIFSLFKEGDKVLVERNCHKSIMNGIIMRKLKPVFINNIFHSKLKAPVGIDLNHLKEQISLNKDLKGIILT
YPNYYGVGVNIDETIKIAREANLKVGIDSAHGAHFGFNKLLPESVQELKADIVVMSAHKTLPSLTQTAWMHVNNEDFIEE
IEFYKNIFMSTSPSYMFMMSLDYSRNFLEEHGEEAYNNLFKIIEDFKNHIKDLSYFKILDKEFLINGLEKNIKINDIKID
NSRIVLNLRENLNGNKLLDYLRSKNIQCEMSDNKNVVLIPSPFNTEEDFKILKDALKSCDINCLKDEEIDFYLTDLPEKK
LEPFEVLDLEYERININDAVGKIVAESIVPYPPGVPMIIMGEVIEERHIKLLNEYMKSKVDLIGIKDKKVKVIKNK

Sequences:

>Translated_476_residues
MRNIPIINGISMYLREEIVPFSMPGHKNGRGFKGFEEILLKGDLTEVEGLDNLQHPEGIIKEAEDKLSNLYKSKKSYFLV
NGSTCGNLIMIFSLFKEGDKVLVERNCHKSIMNGIIMRKLKPVFINNIFHSKLKAPVGIDLNHLKEQISLNKDLKGIILT
YPNYYGVGVNIDETIKIAREANLKVGIDSAHGAHFGFNKLLPESVQELKADIVVMSAHKTLPSLTQTAWMHVNNEDFIEE
IEFYKNIFMSTSPSYMFMMSLDYSRNFLEEHGEEAYNNLFKIIEDFKNHIKDLSYFKILDKEFLINGLEKNIKINDIKID
NSRIVLNLRENLNGNKLLDYLRSKNIQCEMSDNKNVVLIPSPFNTEEDFKILKDALKSCDINCLKDEEIDFYLTDLPEKK
LEPFEVLDLEYERININDAVGKIVAESIVPYPPGVPMIIMGEVIEERHIKLLNEYMKSKVDLIGIKDKKVKVIKNK
>Mature_476_residues
MRNIPIINGISMYLREEIVPFSMPGHKNGRGFKGFEEILLKGDLTEVEGLDNLQHPEGIIKEAEDKLSNLYKSKKSYFLV
NGSTCGNLIMIFSLFKEGDKVLVERNCHKSIMNGIIMRKLKPVFINNIFHSKLKAPVGIDLNHLKEQISLNKDLKGIILT
YPNYYGVGVNIDETIKIAREANLKVGIDSAHGAHFGFNKLLPESVQELKADIVVMSAHKTLPSLTQTAWMHVNNEDFIEE
IEFYKNIFMSTSPSYMFMMSLDYSRNFLEEHGEEAYNNLFKIIEDFKNHIKDLSYFKILDKEFLINGLEKNIKINDIKID
NSRIVLNLRENLNGNKLLDYLRSKNIQCEMSDNKNVVLIPSPFNTEEDFKILKDALKSCDINCLKDEEIDFYLTDLPEKK
LEPFEVLDLEYERININDAVGKIVAESIVPYPPGVPMIIMGEVIEERHIKLLNEYMKSKVDLIGIKDKKVKVIKNK

Specific function: Appears To Play A Role In pH Homeostasis By Consuming Protons And Neutralizing The Acidic By-Products Of Carbohydrate Fermentation. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1790573, Length=546, Percent_Identity=22.5274725274725, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786384, Length=553, Percent_Identity=21.6998191681736, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI221142684, Length=352, Percent_Identity=22.4431818181818, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87082193, Length=301, Percent_Identity=25.5813953488372, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.18 [C]

Molecular weight: Translated: 54716; Mature: 54716

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIPIINGISMYLREEIVPFSMPGHKNGRGFKGFEEILLKGDLTEVEGLDNLQHPEGII
CCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCHHHH
KEAEDKLSNLYKSKKSYFLVNGSTCGNLIMIFSLFKEGDKVLVERNCHKSIMNGIIMRKL
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
KPVFINNIFHSKLKAPVGIDLNHLKEQISLNKDLKGIILTYPNYYGVGVNIDETIKIARE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCEEECCCCHHHHHHHHHH
ANLKVGIDSAHGAHFGFNKLLPESVQELKADIVVMSAHKTLPSLTQTAWMHVNNEDFIEE
CCCEEECCCCCCCCCCHHHHCHHHHHHHHHCEEEEECCCCCCHHHHHHEEECCCHHHHHH
IEFYKNIFMSTSPSYMFMMSLDYSRNFLEEHGEEAYNNLFKIIEDFKNHIKDLSYFKILD
HHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEFLINGLEKNIKINDIKIDNSRIVLNLRENLNGNKLLDYLRSKNIQCEMSDNKNVVLIP
HHHHHHHHHCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCEEEEC
SPFNTEEDFKILKDALKSCDINCLKDEEIDFYLTDLPEKKLEPFEVLDLEYERININDAV
CCCCCHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCEEEEECEEEEECCHHHH
GKIVAESIVPYPPGVPMIIMGEVIEERHIKLLNEYMKSKVDLIGIKDKKVKVIKNK
HHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCC
>Mature Secondary Structure
MRNIPIINGISMYLREEIVPFSMPGHKNGRGFKGFEEILLKGDLTEVEGLDNLQHPEGII
CCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCHHHH
KEAEDKLSNLYKSKKSYFLVNGSTCGNLIMIFSLFKEGDKVLVERNCHKSIMNGIIMRKL
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
KPVFINNIFHSKLKAPVGIDLNHLKEQISLNKDLKGIILTYPNYYGVGVNIDETIKIARE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCEEECCCCHHHHHHHHHH
ANLKVGIDSAHGAHFGFNKLLPESVQELKADIVVMSAHKTLPSLTQTAWMHVNNEDFIEE
CCCEEECCCCCCCCCCHHHHCHHHHHHHHHCEEEEECCCCCCHHHHHHEEECCCHHHHHH
IEFYKNIFMSTSPSYMFMMSLDYSRNFLEEHGEEAYNNLFKIIEDFKNHIKDLSYFKILD
HHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEFLINGLEKNIKINDIKIDNSRIVLNLRENLNGNKLLDYLRSKNIQCEMSDNKNVVLIP
HHHHHHHHHCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCEEEEC
SPFNTEEDFKILKDALKSCDINCLKDEEIDFYLTDLPEKKLEPFEVLDLEYERININDAV
CCCCCHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCEEEEECEEEEECCHHHH
GKIVAESIVPYPPGVPMIIMGEVIEERHIKLLNEYMKSKVDLIGIKDKKVKVIKNK
HHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate; Sulfhydryl groups [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.4 {S-aminoethyl-L-Cys}} 2 {L-Lys}} 1.5 {L-Lys}} 7 {delta-hydroxylysine}} [C]

Substrates: H+; L-Lysine [C]

Specific reaction: H+ + L-Lysine --> 1,5-Diaminopentane + CO2 [C]

General reaction: Decarboxylation [C]

Inhibitor: NaBH4; NaCl [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]