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The map label for this gene is ispD

Identifier: 18311411

GI number: 18311411

Start: 2777857

End: 2778534

Strand: Reverse

Name: ispD

Synonym: CPE2429

Alternate gene names: 18311411

Gene position: 2778534-2777857 (Counterclockwise)

Preceding gene: 18311412

Following gene: 18311410

Centisome position: 91.66

GC content: 29.35

Gene sequence:

>678_bases
ATGGGAAAAGTAGTTAGCGTAATATTAGCAGGTGGAAAAGGAAAAAGAATGGGTGCTGAAGTTAGTAAGCAATTCATTGA
AATAAATGGAAAACCTATAATATATTATACTTTAAAAGCTTTTGAGGAGTGTAAAGGTATTGATGAAATAATTCTTGTTC
TACCTAAGGATGAAATAGATTATTTTAAAAGAGAAATAGAACCTAGATTTGATTTTAAAATATCTAAAATTATTGAAGGT
GGAAAGGAAAGACAGGATTCAGTTTACAATGCATTAAATTCCATAGGAGATTGTGATATTGTTTTAATACATGATGGAGC
AAGAGCTTTTGTAAGCAATAAAATAATAGAAGACGGAATAAAGTATTCTAGAGAGTTTGGAGCAGCAGCTCCAGGTGTTA
TGCCAAAGGACACTATAAAAGTTAAAAATTTAGAAGGATTCTCTGTGGATACACCAAATAGAGCATCTTTAGTAGCTGTT
CAAACTCCACAATGCTTTAAATATAATTTAATAAAAAAAGGTCATAACAAAGTTAAAAATGAAAAGATTCAAGTTACGGA
TGATACTATGATAGTAGAACTTTTAGGAGAAAAAGTTTATCTTTTTGAAGGTGACTATAAAAACATAAAGGTTACAACAC
CAGAAGACTTAATATTAGCAGAACATTTTGTTAAGTAA

Upstream 100 bases:

>100_bases
TACATGGGAAAAGAAATAACAGTAGTTGTAACTTCAGTACTTCAAACAGCAGCAGGAAGAATGATATTTGCTAAATATAA
ATCTTAAGAGGAATTTAATT

Downstream 100 bases:

>100_bases
GGATCACAAGATAGAGACAGTATATTGACATGTTATTTTTATAAGGATATAATTTTATAAGATTTAAGAAAATTTAATAG
TTGTAAAGACTGTGAAAAAG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MGKVVSVILAGGKGKRMGAEVSKQFIEINGKPIIYYTLKAFEECKGIDEIILVLPKDEIDYFKREIEPRFDFKISKIIEG
GKERQDSVYNALNSIGDCDIVLIHDGARAFVSNKIIEDGIKYSREFGAAAPGVMPKDTIKVKNLEGFSVDTPNRASLVAV
QTPQCFKYNLIKKGHNKVKNEKIQVTDDTMIVELLGEKVYLFEGDYKNIKVTTPEDLILAEHFVK

Sequences:

>Translated_225_residues
MGKVVSVILAGGKGKRMGAEVSKQFIEINGKPIIYYTLKAFEECKGIDEIILVLPKDEIDYFKREIEPRFDFKISKIIEG
GKERQDSVYNALNSIGDCDIVLIHDGARAFVSNKIIEDGIKYSREFGAAAPGVMPKDTIKVKNLEGFSVDTPNRASLVAV
QTPQCFKYNLIKKGHNKVKNEKIQVTDDTMIVELLGEKVYLFEGDYKNIKVTTPEDLILAEHFVK
>Mature_224_residues
GKVVSVILAGGKGKRMGAEVSKQFIEINGKPIIYYTLKAFEECKGIDEIILVLPKDEIDYFKREIEPRFDFKISKIIEGG
KERQDSVYNALNSIGDCDIVLIHDGARAFVSNKIIEDGIKYSREFGAAAPGVMPKDTIKVKNLEGFSVDTPNRASLVAVQ
TPQCFKYNLIKKGHNKVKNEKIQVTDDTMIVELLGEKVYLFEGDYKNIKVTTPEDLILAEHFVK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=233, Percent_Identity=27.4678111587983, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI157671913, Length=135, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1789104, Length=228, Percent_Identity=30.7017543859649, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CLOP1 (Q0TMM2)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_697118.1
- ProteinModelPortal:   Q0TMM2
- SMR:   Q0TMM2
- STRING:   Q0TMM2
- GeneID:   4202885
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2739
- TIGR:   CPF_2739
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   ALSIVHT
- ProtClustDB:   PRK00155
- BioCyc:   CPER195103:CPF_2739-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25250; Mature: 25118

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVVSVILAGGKGKRMGAEVSKQFIEINGKPIIYYTLKAFEECKGIDEIILVLPKDEID
CCCEEEEEEECCCCCCCCHHHHHHHHEECCCEEEEEEHHHHHHHCCCCCEEEEECCCHHH
YFKREIEPRFDFKISKIIEGGKERQDSVYNALNSIGDCDIVLIHDGARAFVSNKIIEDGI
HHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHH
KYSREFGAAAPGVMPKDTIKVKNLEGFSVDTPNRASLVAVQTPQCFKYNLIKKGHNKVKN
HHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHCC
EKIQVTDDTMIVELLGEKVYLFEGDYKNIKVTTPEDLILAEHFVK
CEEEEECCHHEEEECCCEEEEEECCCCEEEEECCHHHHHHHHHCC
>Mature Secondary Structure 
GKVVSVILAGGKGKRMGAEVSKQFIEINGKPIIYYTLKAFEECKGIDEIILVLPKDEID
CCEEEEEEECCCCCCCCHHHHHHHHEECCCEEEEEEHHHHHHHCCCCCEEEEECCCHHH
YFKREIEPRFDFKISKIIEGGKERQDSVYNALNSIGDCDIVLIHDGARAFVSNKIIEDGI
HHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHH
KYSREFGAAAPGVMPKDTIKVKNLEGFSVDTPNRASLVAVQTPQCFKYNLIKKGHNKVKN
HHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHCC
EKIQVTDDTMIVELLGEKVYLFEGDYKNIKVTTPEDLILAEHFVK
CEEEEECCHHEEEECCCEEEEEECCCCEEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA