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The map label for this gene is proS [H]

Identifier: 18311410

GI number: 18311410

Start: 2775790

End: 2777502

Strand: Reverse

Name: proS [H]

Synonym: CPE2428

Alternate gene names: 18311410

Gene position: 2777502-2775790 (Counterclockwise)

Preceding gene: 18311411

Following gene: 18311409

Centisome position: 91.62

GC content: 32.92

Gene sequence:

>1713_bases
ATGAAAATGTCAAATATGTTAGTAGGAACTTTAAGAGAAGTTCCAGCTGAAGCAGAAATAGAAAGTCACAAGCTTATGCT
TAGAGCAGGTCTTATGAGAAAGATGGCTGCAGGTATTTATAACTATATGCCTTTAGGATTAAAGGTTATAGAAAATGTTA
AAAATATAGTAAGAGAAGAAATGAATAATGCAGGTGCTCAAGAATTCTTAGCATCAGCTTTAATACCAGCTGAGTTATGG
CAAGAATCAGGAAGATGGGATGCTTATGGAGCAGAAATGTTTAGATTAAAAGATAGACATAACAGAGATTTTTGCTTAGG
ACCAACTCACGAAGAGGTATTTACTGATATAGTTAGAAATGAAATAAAATCATATAAGCAATTACCATTAAATCTTTATC
AAATACAAACTAAGTATAGAGATGAAAGAAGACCAAGATTTGGAGTTATGAGATCAAGAGAATTCATAATGAAAGATGGA
TATAGCTTTGACAAAGATCAAGAAGGATTAGATTTAGCATATGAAAAAATGAGAAAAGCATATGTTAATATATTTAATAG
ATGTGGATTAGATGCTAAGGCAGTTGCAGCTGATTCAGGAGCTATAGGTGGATCAGGTTCAGCTGAGTTTATGGTTAAAT
CAGAAGTGGGAGAAGACGATGTAGTATTCTGTACAGCTTGTGATTATGCAGCTAACATAGAAAAAGCTCCATCAACACCA
GAACATGGAGAAAAAGAAGAGTTAATGGAAGTAGAAAAAGTTGAAACTCCAGCTGTTAAATCAATTGAAGATTTAGCAAA
ATTCTTTGAATGCTCACCAAAGAAAATAGCAAAAACTTTAATATTCCAAGCTGATGATAAAGTTGTTGCTGTTGTATTAA
GAGGAGATAGAGAAGCTAACGAAGTTAAGATAGCTAATGCTATTGGAGAAGTTATAGAATTAGAAATGGCAAGCGAAGAG
GCTGTTAAAGAAGCTACTGGCGCAGCTGTTGGATTTGCAGGTCCTATGGGAATAAAAGTAGATATGTTATTAGTTGACCA
AGAAGTAGCTAATATGTATAACTTCATAATTGGTGCTAATGAAACTGATATGCACTTAAAAAATGTAAACTATGGAAGAG
ACTTTGAAGGAATAGTTGGTGACTTTAGAAATGTTACTATAGGAGAAAAATGTCCTGAGTGTGGAAAAGAAATAACTATT
TCAAGAGGTACTGAGGTTGGACATATATTTAAACTTGGAACTAAGTATTCAGAGTCTATGGGTGCAACATTTATTGATGA
AGATGGAAAAGCTAAACCATTTATAATGGGATGCTATGGAATAGGGGTTACAAGAACTGTAGCTTCAATAATAGAGCAAC
ACAATGACGAAAACGGAATAATATGGCCATTAGAAGTAGCTCCATACCATGTATCAGTTATACCAGCTAATGTTAAAAAT
GAAGAACAAGCAACTAAAGCTGAAGAAATATACAATGAATTAAGAAAAATGGGAGTTGAAGCTCTACTTGATGATAGAAA
AGAAAGAGCAGGAGTTAAATTCAAAGATTCTGAATTAATGGGAATTCCAATGAGAATAACTGTTGGAAAGATGATTGGTG
AAGGTCAAGTTGAATTTAAACTTAGAAATGGTGGAGAAGTTGAGACTTTATCTATAGAAGAAGTTTATAATAGAGTAAGA
GAAGAATTTGAAAGAGCAAATTTATCTTTATAA

Upstream 100 bases:

>100_bases
CTCGCCCTAAAGTTTTTTTAGGGTAGAGCTTTTTTTATTTTTTTAATGAAATTAATTAAAATATTAAGATTATAAATAGC
TTATGAGGAGGTAGTATATT

Downstream 100 bases:

>100_bases
TTTATAAGATTAACGGAATTGTATATAATTAGAATATAATAATAGAGCTCACCTTATTTAATGTAGGGTGAGTTACTAAT
AATATAAGGTAGGAGGGTCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MKMSNMLVGTLREVPAEAEIESHKLMLRAGLMRKMAAGIYNYMPLGLKVIENVKNIVREEMNNAGAQEFLASALIPAELW
QESGRWDAYGAEMFRLKDRHNRDFCLGPTHEEVFTDIVRNEIKSYKQLPLNLYQIQTKYRDERRPRFGVMRSREFIMKDG
YSFDKDQEGLDLAYEKMRKAYVNIFNRCGLDAKAVAADSGAIGGSGSAEFMVKSEVGEDDVVFCTACDYAANIEKAPSTP
EHGEKEELMEVEKVETPAVKSIEDLAKFFECSPKKIAKTLIFQADDKVVAVVLRGDREANEVKIANAIGEVIELEMASEE
AVKEATGAAVGFAGPMGIKVDMLLVDQEVANMYNFIIGANETDMHLKNVNYGRDFEGIVGDFRNVTIGEKCPECGKEITI
SRGTEVGHIFKLGTKYSESMGATFIDEDGKAKPFIMGCYGIGVTRTVASIIEQHNDENGIIWPLEVAPYHVSVIPANVKN
EEQATKAEEIYNELRKMGVEALLDDRKERAGVKFKDSELMGIPMRITVGKMIGEGQVEFKLRNGGEVETLSIEEVYNRVR
EEFERANLSL

Sequences:

>Translated_570_residues
MKMSNMLVGTLREVPAEAEIESHKLMLRAGLMRKMAAGIYNYMPLGLKVIENVKNIVREEMNNAGAQEFLASALIPAELW
QESGRWDAYGAEMFRLKDRHNRDFCLGPTHEEVFTDIVRNEIKSYKQLPLNLYQIQTKYRDERRPRFGVMRSREFIMKDG
YSFDKDQEGLDLAYEKMRKAYVNIFNRCGLDAKAVAADSGAIGGSGSAEFMVKSEVGEDDVVFCTACDYAANIEKAPSTP
EHGEKEELMEVEKVETPAVKSIEDLAKFFECSPKKIAKTLIFQADDKVVAVVLRGDREANEVKIANAIGEVIELEMASEE
AVKEATGAAVGFAGPMGIKVDMLLVDQEVANMYNFIIGANETDMHLKNVNYGRDFEGIVGDFRNVTIGEKCPECGKEITI
SRGTEVGHIFKLGTKYSESMGATFIDEDGKAKPFIMGCYGIGVTRTVASIIEQHNDENGIIWPLEVAPYHVSVIPANVKN
EEQATKAEEIYNELRKMGVEALLDDRKERAGVKFKDSELMGIPMRITVGKMIGEGQVEFKLRNGGEVETLSIEEVYNRVR
EEFERANLSL
>Mature_570_residues
MKMSNMLVGTLREVPAEAEIESHKLMLRAGLMRKMAAGIYNYMPLGLKVIENVKNIVREEMNNAGAQEFLASALIPAELW
QESGRWDAYGAEMFRLKDRHNRDFCLGPTHEEVFTDIVRNEIKSYKQLPLNLYQIQTKYRDERRPRFGVMRSREFIMKDG
YSFDKDQEGLDLAYEKMRKAYVNIFNRCGLDAKAVAADSGAIGGSGSAEFMVKSEVGEDDVVFCTACDYAANIEKAPSTP
EHGEKEELMEVEKVETPAVKSIEDLAKFFECSPKKIAKTLIFQADDKVVAVVLRGDREANEVKIANAIGEVIELEMASEE
AVKEATGAAVGFAGPMGIKVDMLLVDQEVANMYNFIIGANETDMHLKNVNYGRDFEGIVGDFRNVTIGEKCPECGKEITI
SRGTEVGHIFKLGTKYSESMGATFIDEDGKAKPFIMGCYGIGVTRTVASIIEQHNDENGIIWPLEVAPYHVSVIPANVKN
EEQATKAEEIYNELRKMGVEALLDDRKERAGVKFKDSELMGIPMRITVGKMIGEGQVEFKLRNGGEVETLSIEEVYNRVR
EEFERANLSL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=228, Percent_Identity=42.5438596491228, Blast_Score=215, Evalue=1e-55,
Organism=Escherichia coli, GI1786392, Length=554, Percent_Identity=49.8194945848375, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1788013, Length=185, Percent_Identity=24.3243243243243, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115532348, Length=210, Percent_Identity=40, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=34.1176470588235, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=517, Percent_Identity=32.495164410058, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24656200, Length=237, Percent_Identity=37.5527426160338, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63745; Mature: 63745

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMSNMLVGTLREVPAEAEIESHKLMLRAGLMRKMAAGIYNYMPLGLKVIENVKNIVREE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAQEFLASALIPAELWQESGRWDAYGAEMFRLKDRHNRDFCLGPTHEEVFTDIVRN
HHCCCHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
EIKSYKQLPLNLYQIQTKYRDERRPRFGVMRSREFIMKDGYSFDKDQEGLDLAYEKMRKA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
YVNIFNRCGLDAKAVAADSGAIGGSGSAEFMVKSEVGEDDVVFCTACDYAANIEKAPSTP
HHHHHHHCCCCHHHEECCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCHHCCCCCCCCC
EHGEKEELMEVEKVETPAVKSIEDLAKFFECSPKKIAKTLIFQADDKVVAVVLRGDREAN
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHEECCCCEEEEEEECCCCCC
EVKIANAIGEVIELEMASEEAVKEATGAAVGFAGPMGIKVDMLLVDQEVANMYNFIIGAN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEECHHHHHHHHHHCCCC
ETDMHLKNVNYGRDFEGIVGDFRNVTIGEKCPECGKEITISRGTEVGHIFKLGTKYSESM
CCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHEECCCHHHHHC
GATFIDEDGKAKPFIMGCYGIGVTRTVASIIEQHNDENGIIWPLEVAPYHVSVIPANVKN
CCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCC
EEQATKAEEIYNELRKMGVEALLDDRKERAGVKFKDSELMGIPMRITVGKMIGEGQVEFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEECCCEEEEECHHCCCCEEEEE
LRNGGEVETLSIEEVYNRVREEFERANLSL
ECCCCCEEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKMSNMLVGTLREVPAEAEIESHKLMLRAGLMRKMAAGIYNYMPLGLKVIENVKNIVREE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAQEFLASALIPAELWQESGRWDAYGAEMFRLKDRHNRDFCLGPTHEEVFTDIVRN
HHCCCHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
EIKSYKQLPLNLYQIQTKYRDERRPRFGVMRSREFIMKDGYSFDKDQEGLDLAYEKMRKA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
YVNIFNRCGLDAKAVAADSGAIGGSGSAEFMVKSEVGEDDVVFCTACDYAANIEKAPSTP
HHHHHHHCCCCHHHEECCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCHHCCCCCCCCC
EHGEKEELMEVEKVETPAVKSIEDLAKFFECSPKKIAKTLIFQADDKVVAVVLRGDREAN
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHEECCCCEEEEEEECCCCCC
EVKIANAIGEVIELEMASEEAVKEATGAAVGFAGPMGIKVDMLLVDQEVANMYNFIIGAN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEECHHHHHHHHHHCCCC
ETDMHLKNVNYGRDFEGIVGDFRNVTIGEKCPECGKEITISRGTEVGHIFKLGTKYSESM
CCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHEECCCHHHHHC
GATFIDEDGKAKPFIMGCYGIGVTRTVASIIEQHNDENGIIWPLEVAPYHVSVIPANVKN
CCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCC
EEQATKAEEIYNELRKMGVEALLDDRKERAGVKFKDSELMGIPMRITVGKMIGEGQVEFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEECCCEEEEECHHCCCCEEEEE
LRNGGEVETLSIEEVYNRVREEFERANLSL
ECCCCCEEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA