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The map label for this gene is prfB [H]

Identifier: 18311152

GI number: 18311152

Start: 2488191

End: 2489162

Strand: Reverse

Name: prfB [H]

Synonym: CPE2170

Alternate gene names: 18311152

Gene position: 2489162-2488191 (Counterclockwise)

Preceding gene: 18311153

Following gene: 18311150

Centisome position: 82.11

GC content: 32.61

Gene sequence:

>972_bases
ATGCAAGAAGCGGGATTTTGGGATGATGTCCAAAAGGCCCAAGAAGTAACCCAAGAAGCAAAAAGAGTAAAAGATAAAAT
TGATAAGTTTAGAAATTTAAATGAAAGAATAGATGATGTAGAAGTATTAAAAGAGCTTATGGAAGAAAATGACGAGGAAA
CAGCTAAGGAGATAATAAGCGAAGTTAAGGCTTTAAGTAAAGAGATAGATACATTAAAAATAGAGACAATTCTTTCTGGG
GAATATGATAGAAATGATGCCATATTAACACTTCATACAGGTGTTGGTGGAAGCGATGCTAATGATTGGACAGAAATGCT
TCTTAGAATGTACACAAGATGGTGTGAAAAGAAGGGATATTCCTTAGAAACTATTGATTATTTACCTGGAGATGAAGCTG
GAGTTAAAAGTGTTACTTTAAAAGTTAAAGGTGAATTTGCTTATGGATATTTAAAAGCTGAAAAGGGTATTCACAGATTA
GTTAGAATATCACCTTTTAATGCTAATGGTAAAAGACAAACTTCCTTTGCTTCAGTAGAAGTTTTACCAGAGTTAACATC
TGATCAAGATATAGAAATAAATCCTGTAGACTTAAGAATAGACACTTATAGAGCAGGAGGAGCTGGTGGTCAGCATGTAA
ATAAAACTGAGTCAGCAGTAAGAATTACTCATATTCCAACTGGAATAGTAGTTCAATGCCAAAATGAGAGAAGTCAGTTT
TCTAATAGAGATACTGCTATGGGAATGTTAAAGTCTAAACTTATAGAACTTAAAGAGAGAGCTCATAAGGAAAAAATAGA
GGATTTAACAGGTGAATTAAAAGATATGGGATGGGGAAGTCAGATAAGAAGTTATGTTTTTCATCCATATTCAATGGTTA
AGGATCATAGAACTAATGTTGAGACATCTAATGTGAACGGAGTAATGGATGGAGATATAGATAATTTTATAATTGCCTAT
TTAAATTCATAA

Upstream 100 bases:

>100_bases
TTATCAAAGTTACATGAACTAAAAAATAATTTAAAGGAAATGGGGGCTTCACTTTGACTTAGCATCTTTAGAGAAGAAGA
GTGCAGAATTAGAGCTTAAG

Downstream 100 bases:

>100_bases
AAAGGTGACTATATAAAAAACTTATAAATAGATTATGAGAATTAATGAATTTGAATTAAGAATTTTAAGTAAGAACTTTG
AAAATATATTAAAAACTATT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MQEAGFWDDVQKAQEVTQEAKRVKDKIDKFRNLNERIDDVEVLKELMEENDEETAKEIISEVKALSKEIDTLKIETILSG
EYDRNDAILTLHTGVGGSDANDWTEMLLRMYTRWCEKKGYSLETIDYLPGDEAGVKSVTLKVKGEFAYGYLKAEKGIHRL
VRISPFNANGKRQTSFASVEVLPELTSDQDIEINPVDLRIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQF
SNRDTAMGMLKSKLIELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYSMVKDHRTNVETSNVNGVMDGDIDNFIIAY
LNS

Sequences:

>Translated_323_residues
MQEAGFWDDVQKAQEVTQEAKRVKDKIDKFRNLNERIDDVEVLKELMEENDEETAKEIISEVKALSKEIDTLKIETILSG
EYDRNDAILTLHTGVGGSDANDWTEMLLRMYTRWCEKKGYSLETIDYLPGDEAGVKSVTLKVKGEFAYGYLKAEKGIHRL
VRISPFNANGKRQTSFASVEVLPELTSDQDIEINPVDLRIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQF
SNRDTAMGMLKSKLIELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYSMVKDHRTNVETSNVNGVMDGDIDNFIIAY
LNS
>Mature_323_residues
MQEAGFWDDVQKAQEVTQEAKRVKDKIDKFRNLNERIDDVEVLKELMEENDEETAKEIISEVKALSKEIDTLKIETILSG
EYDRNDAILTLHTGVGGSDANDWTEMLLRMYTRWCEKKGYSLETIDYLPGDEAGVKSVTLKVKGEFAYGYLKAEKGIHRL
VRISPFNANGKRQTSFASVEVLPELTSDQDIEINPVDLRIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQF
SNRDTAMGMLKSKLIELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYSMVKDHRTNVETSNVNGVMDGDIDNFIIAY
LNS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=290, Percent_Identity=38.2758620689655, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI34577120, Length=302, Percent_Identity=34.7682119205298, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI166795305, Length=199, Percent_Identity=39.6984924623116, Blast_Score=141, Evalue=8e-34,
Organism=Escherichia coli, GI2367172, Length=327, Percent_Identity=49.5412844036697, Blast_Score=325, Evalue=2e-90,
Organism=Escherichia coli, GI1787462, Length=314, Percent_Identity=34.7133757961783, Blast_Score=181, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=310, Percent_Identity=29.3548387096774, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=301, Percent_Identity=28.9036544850498, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI19921226, Length=273, Percent_Identity=35.8974358974359, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36601; Mature: 36601

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEAGFWDDVQKAQEVTQEAKRVKDKIDKFRNLNERIDDVEVLKELMEENDEETAKEIIS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHH
EVKALSKEIDTLKIETILSGEYDRNDAILTLHTGVGGSDANDWTEMLLRMYTRWCEKKGY
HHHHHHHHHHHEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
SLETIDYLPGDEAGVKSVTLKVKGEFAYGYLKAEKGIHRLVRISPFNANGKRQTSFASVE
CEEEEECCCCCCCCCEEEEEEEECCEEEEEHHHHHHHHHHEEECCCCCCCCCCCHHHHHH
VLPELTSDQDIEINPVDLRIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQF
CCCCCCCCCCCEECEEEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHH
SNRDTAMGMLKSKLIELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYSMVKDHRTNV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCC
ETSNVNGVMDGDIDNFIIAYLNS
CCCCCCCEECCCCCCEEEEEECC
>Mature Secondary Structure
MQEAGFWDDVQKAQEVTQEAKRVKDKIDKFRNLNERIDDVEVLKELMEENDEETAKEIIS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHH
EVKALSKEIDTLKIETILSGEYDRNDAILTLHTGVGGSDANDWTEMLLRMYTRWCEKKGY
HHHHHHHHHHHEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
SLETIDYLPGDEAGVKSVTLKVKGEFAYGYLKAEKGIHRLVRISPFNANGKRQTSFASVE
CEEEEECCCCCCCCCEEEEEEEECCEEEEEHHHHHHHHHHEEECCCCCCCCCCCHHHHHH
VLPELTSDQDIEINPVDLRIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQF
CCCCCCCCCCCEECEEEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHH
SNRDTAMGMLKSKLIELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYSMVKDHRTNV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCC
ETSNVNGVMDGDIDNFIIAYLNS
CCCCCCCEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA