Click here to switch to the map view.

The map label for this gene is virJ [H]

Identifier: 18311080

GI number: 18311080

Start: 2406617

End: 2408065

Strand: Reverse

Name: virJ [H]

Synonym: CPE2098

Alternate gene names: 18311080

Gene position: 2408065-2406617 (Counterclockwise)

Preceding gene: 18311081

Following gene: 18311079

Centisome position: 79.44

GC content: 25.05

Gene sequence:

>1449_bases
ATGAAAGTTAAACATAAATTCTCAGGAGGATTTTTAATAATATTTCTAATTGCCTTTATAATTTTAAACTGTTTTTTAGG
TAAAGCTTTTATTAATTATCATGAAAATAAAATTAAAAATGAAATGGATTCTTTATATAAAAGTAGCTACAGTATTTTAA
GGTTATATTTTCAAGTAAACAATTTGGAAGGAAACAAGGGTAACTTTAATAGATATATTCCTAAAATATCAAATGATATT
AGTGAACAAGGAAAGTGCCAAATTGATGTTTTTAATGAAGCTTTAGAAAAGGAATATAGTTATTATTTAGATGATTTTTC
CTTTGATTACGAAAAGGATGGGGGCTATGATGTTTTAAAAGAAGCAAATAAGAATAAGGCTGCATTAAACATTTATAGAG
ATGAAAATGCTGTTAAAGCATACTTAGCTTTTCCAGTTTATGTTGATGAGAGTTTTTTAGGAAATATACTTTTAAGCAAG
GATTATTCAAAGGAATATTTAGAGTTAAGAGATTTAATGAGCAATATAAAATTCATTGTTTCAATAACATTTTTACTTAT
ATTAATTTTCACATACTTTCTTTTTGAGAGAATAATAAATCCTTTAAATTATATAAAAAATTCTTTTAAAAGAATTGGTG
ATGGTGACTTTAAAATAAACTTAAAAGTTAATTCAAAGGATGAAATAGGAGATTTAGCCTGTGGAGTAAATAAAATGGCA
AATAAAATTGAGGAACAGATTGAAAAAATAAATGAAGAAAAGGAAAAGGTTTTAACATTAGAAAAAACTAGAACAGAGTT
TTTTAATAATATTACTCATGAGTTAAAAACTCCATTAACTAATATTTCAGGATATGCTCAAATTATGAGTGAAGAAGATT
TTCATGATAATGATTTTAGAAAATTTGCTTTAGAGAGAATAAACAAGGAAAGCAAAAGAATGCATGAACTTATAGTAAGT
TTAATTGAAATTTCTAAAAACAAAAGCGACATAGAAGGTGGAGAAATAGAAAATGTAAACCTAAAAACATTAATAGAGGA
TTTATGTGAAGATTTTAAAGTTAAGGGAGATAAACAAGGGATATGCATTTCTAATAATTTAGAAGATATTGAGTTTATGT
GTAGAGAAGATGAAATAAGGAGAATTATTATAAATCTTTTAGATAATGCCATAAAGTATTCAATAAAGGGTAGTGAAATA
AATATTAGTCTATTTAAAGATAAGGAAGAAAAACATATAGAGATAGAGAATTTTAGTGATGAGATTAGTAGTGAGGGAGT
AGAAAAAATATTTGAGCCATTTTATAGAGAGAATACTAAAAAATCTAGAGAGTTAGGGGGAAATGGTTTAGGACTTTATA
TATGCTCAGAGCTAGTAAAAAAATATGGAGGAGATATTTCCTTTAGCTATTCTAACGGGAAGGTAAAGGTTAGTATTATT
TTTAAATAA

Upstream 100 bases:

>100_bases
ACTGTTGATGTTCATGTAAGAAGACTAAGACAAAAGCTTAATAATGAGGAAATAATAGAAACTATTTTTGGATTAGGATA
TATGTTTAAAGGTGAGAAAC

Downstream 100 bases:

>100_bases
TTTATATATTTAATTTTGGAAATATTTTGGCAATAAGATTTAATTTTTTAGAAAAAACTCCTGTGTAAAATAAAAGCAAA
TAGTAATTTTATAGAAGATT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKVKHKFSGGFLIIFLIAFIILNCFLGKAFINYHENKIKNEMDSLYKSSYSILRLYFQVNNLEGNKGNFNRYIPKISNDI
SEQGKCQIDVFNEALEKEYSYYLDDFSFDYEKDGGYDVLKEANKNKAALNIYRDENAVKAYLAFPVYVDESFLGNILLSK
DYSKEYLELRDLMSNIKFIVSITFLLILIFTYFLFERIINPLNYIKNSFKRIGDGDFKINLKVNSKDEIGDLACGVNKMA
NKIEEQIEKINEEKEKVLTLEKTRTEFFNNITHELKTPLTNISGYAQIMSEEDFHDNDFRKFALERINKESKRMHELIVS
LIEISKNKSDIEGGEIENVNLKTLIEDLCEDFKVKGDKQGICISNNLEDIEFMCREDEIRRIIINLLDNAIKYSIKGSEI
NISLFKDKEEKHIEIENFSDEISSEGVEKIFEPFYRENTKKSRELGGNGLGLYICSELVKKYGGDISFSYSNGKVKVSII
FK

Sequences:

>Translated_482_residues
MKVKHKFSGGFLIIFLIAFIILNCFLGKAFINYHENKIKNEMDSLYKSSYSILRLYFQVNNLEGNKGNFNRYIPKISNDI
SEQGKCQIDVFNEALEKEYSYYLDDFSFDYEKDGGYDVLKEANKNKAALNIYRDENAVKAYLAFPVYVDESFLGNILLSK
DYSKEYLELRDLMSNIKFIVSITFLLILIFTYFLFERIINPLNYIKNSFKRIGDGDFKINLKVNSKDEIGDLACGVNKMA
NKIEEQIEKINEEKEKVLTLEKTRTEFFNNITHELKTPLTNISGYAQIMSEEDFHDNDFRKFALERINKESKRMHELIVS
LIEISKNKSDIEGGEIENVNLKTLIEDLCEDFKVKGDKQGICISNNLEDIEFMCREDEIRRIIINLLDNAIKYSIKGSEI
NISLFKDKEEKHIEIENFSDEISSEGVEKIFEPFYRENTKKSRELGGNGLGLYICSELVKKYGGDISFSYSNGKVKVSII
FK
>Mature_482_residues
MKVKHKFSGGFLIIFLIAFIILNCFLGKAFINYHENKIKNEMDSLYKSSYSILRLYFQVNNLEGNKGNFNRYIPKISNDI
SEQGKCQIDVFNEALEKEYSYYLDDFSFDYEKDGGYDVLKEANKNKAALNIYRDENAVKAYLAFPVYVDESFLGNILLSK
DYSKEYLELRDLMSNIKFIVSITFLLILIFTYFLFERIINPLNYIKNSFKRIGDGDFKINLKVNSKDEIGDLACGVNKMA
NKIEEQIEKINEEKEKVLTLEKTRTEFFNNITHELKTPLTNISGYAQIMSEEDFHDNDFRKFALERINKESKRMHELIVS
LIEISKNKSDIEGGEIENVNLKTLIEDLCEDFKVKGDKQGICISNNLEDIEFMCREDEIRRIIINLLDNAIKYSIKGSEI
NISLFKDKEEKHIEIENFSDEISSEGVEKIFEPFYRENTKKSRELGGNGLGLYICSELVKKYGGDISFSYSNGKVKVSII
FK

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=24.7386759581882, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=25.8333333333333, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789149, Length=332, Percent_Identity=25, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081816, Length=322, Percent_Identity=25.1552795031056, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082128, Length=297, Percent_Identity=21.2121212121212, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790346, Length=271, Percent_Identity=23.9852398523985, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788713, Length=247, Percent_Identity=23.0769230769231, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786783, Length=315, Percent_Identity=20.3174603174603, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789808, Length=295, Percent_Identity=23.0508474576271, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI145693157, Length=235, Percent_Identity=24.2553191489362, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789403, Length=295, Percent_Identity=22.0338983050847, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787894, Length=464, Percent_Identity=20.0431034482759, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994928, Length=226, Percent_Identity=24.7787610619469, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=23.1111111111111, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=24.4541484716157, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787374, Length=309, Percent_Identity=21.6828478964401, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788279, Length=248, Percent_Identity=24.1935483870968, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56005; Mature: 56005

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKHKFSGGFLIIFLIAFIILNCFLGKAFINYHENKIKNEMDSLYKSSYSILRLYFQVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
NLEGNKGNFNRYIPKISNDISEQGKCQIDVFNEALEKEYSYYLDDFSFDYEKDGGYDVLK
CCCCCCCCCHHHCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
EANKNKAALNIYRDENAVKAYLAFPVYVDESFLGNILLSKDYSKEYLELRDLMSNIKFIV
HCCCCCEEEEEEECCCCEEEEEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
SITFLLILIFTYFLFERIINPLNYIKNSFKRIGDGDFKINLKVNSKDEIGDLACGVNKMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHH
NKIEEQIEKINEEKEKVLTLEKTRTEFFNNITHELKTPLTNISGYAQIMSEEDFHDNDFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
KFALERINKESKRMHELIVSLIEISKNKSDIEGGEIENVNLKTLIEDLCEDFKVKGDKQG
HHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
ICISNNLEDIEFMCREDEIRRIIINLLDNAIKYSIKGSEINISLFKDKEEKHIEIENFSD
EEECCCCHHHHHHHCHHHHHHHHHHHHHHHHEEEECCCEEEEEEEECCCCCEEEECCCHH
EISSEGVEKIFEPFYRENTKKSRELGGNGLGLYICSELVKKYGGDISFSYSNGKVKVSII
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEEE
FK
EC
>Mature Secondary Structure
MKVKHKFSGGFLIIFLIAFIILNCFLGKAFINYHENKIKNEMDSLYKSSYSILRLYFQVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
NLEGNKGNFNRYIPKISNDISEQGKCQIDVFNEALEKEYSYYLDDFSFDYEKDGGYDVLK
CCCCCCCCCHHHCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
EANKNKAALNIYRDENAVKAYLAFPVYVDESFLGNILLSKDYSKEYLELRDLMSNIKFIV
HCCCCCEEEEEEECCCCEEEEEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
SITFLLILIFTYFLFERIINPLNYIKNSFKRIGDGDFKINLKVNSKDEIGDLACGVNKMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHH
NKIEEQIEKINEEKEKVLTLEKTRTEFFNNITHELKTPLTNISGYAQIMSEEDFHDNDFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
KFALERINKESKRMHELIVSLIEISKNKSDIEGGEIENVNLKTLIEDLCEDFKVKGDKQG
HHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
ICISNNLEDIEFMCREDEIRRIIINLLDNAIKYSIKGSEINISLFKDKEEKHIEIENFSD
EEECCCCHHHHHHHCHHHHHHHHHHHHHHHHEEEECCCEEEEEEEECCCCCEEEECCCHH
EISSEGVEKIFEPFYRENTKKSRELGGNGLGLYICSELVKKYGGDISFSYSNGKVKVSII
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEEE
FK
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]