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The map label for this gene is grpE [H]

Identifier: 18311016

GI number: 18311016

Start: 2325672

End: 2326298

Strand: Reverse

Name: grpE [H]

Synonym: CPE2034

Alternate gene names: 18311016

Gene position: 2326298-2325672 (Counterclockwise)

Preceding gene: 18311017

Following gene: 18311015

Centisome position: 76.74

GC content: 30.3

Gene sequence:

>627_bases
ATGGTGGATAATAAAGATTTTAACGAAGAGTTAAAGGAAAATATCCAAGAAGAATTAGATAACGAAACAAAAGCTGAAAA
TCCTAATATTGATGAAGAGGTAGAAGAAGTTTCAGAAGATATTAAGGCTGATGAAAAAGTTATTGATTTTGAAGAATTAC
AGGCTTTAAAGGAAGAAAATACTATGTTTAAAAGTAAAACAAAGAAATTAGAAAATGAATTAGAGGCCTTAAAAGATAGA
TTATTAAGAATATCTGCAGAGTATGAAAATTACAGAAAAAGAACAGATAAAGAAAAAGAAAGAATATATACTGATGCATG
TGAAGATGTTTTAATCAAAATGCTTCCAGTATTAGATAACTTAGAAAGAGCCTTAGCTGTAGATGGAACTGTTGAAGATC
TTAAAAAAGGTGTTGAAATGACAGTAAGACAATTTGAGGATGCTTTAGAAAAACTTCAAGTTGAAGAAATATCTACAGAA
AATGGTTTTGATCCAGAATTACATCAAGCAATGATGGTTGTGGAACAAGAAGGCGCAGAGCCAAATCAAGTAGCTCAAGT
ATTCCAAAAAGGATACAAGAGAGGGGATAAGGTTATCAGACATTCAATGGTAACTGTAACTAAATAA

Upstream 100 bases:

>100_bases
TTATTCTAAAGTCTTATCAATCATGACTGAAGTTATGAAAGAGTTAAATGAAACTTTAAAGAATAAGTAAGTAAAAAAAG
AGAAGAGGTGTGGCTGCTTT

Downstream 100 bases:

>100_bases
TTTAATTAGCATTTAACAGGTTTAAATTATAGATATTGTTAGAGGAGGAATTAACAATGAGTAAAATAATCGGTATAGAT
TTAGGAACAACAAATTCATG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MVDNKDFNEELKENIQEELDNETKAENPNIDEEVEEVSEDIKADEKVIDFEELQALKEENTMFKSKTKKLENELEALKDR
LLRISAEYENYRKRTDKEKERIYTDACEDVLIKMLPVLDNLERALAVDGTVEDLKKGVEMTVRQFEDALEKLQVEEISTE
NGFDPELHQAMMVVEQEGAEPNQVAQVFQKGYKRGDKVIRHSMVTVTK

Sequences:

>Translated_208_residues
MVDNKDFNEELKENIQEELDNETKAENPNIDEEVEEVSEDIKADEKVIDFEELQALKEENTMFKSKTKKLENELEALKDR
LLRISAEYENYRKRTDKEKERIYTDACEDVLIKMLPVLDNLERALAVDGTVEDLKKGVEMTVRQFEDALEKLQVEEISTE
NGFDPELHQAMMVVEQEGAEPNQVAQVFQKGYKRGDKVIRHSMVTVTK
>Mature_208_residues
MVDNKDFNEELKENIQEELDNETKAENPNIDEEVEEVSEDIKADEKVIDFEELQALKEENTMFKSKTKKLENELEALKDR
LLRISAEYENYRKRTDKEKERIYTDACEDVLIKMLPVLDNLERALAVDGTVEDLKKGVEMTVRQFEDALEKLQVEEISTE
NGFDPELHQAMMVVEQEGAEPNQVAQVFQKGYKRGDKVIRHSMVTVTK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=136, Percent_Identity=38.9705882352941, Blast_Score=94, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324806, Length=161, Percent_Identity=32.9192546583851, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 24201; Mature: 24201

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDNKDFNEELKENIQEELDNETKAENPNIDEEVEEVSEDIKADEKVIDFEELQALKEEN
CCCCCCHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TMFKSKTKKLENELEALKDRLLRISAEYENYRKRTDKEKERIYTDACEDVLIKMLPVLDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LERALAVDGTVEDLKKGVEMTVRQFEDALEKLQVEEISTENGFDPELHQAMMVVEQEGAE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
PNQVAQVFQKGYKRGDKVIRHSMVTVTK
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVDNKDFNEELKENIQEELDNETKAENPNIDEEVEEVSEDIKADEKVIDFEELQALKEEN
CCCCCCHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TMFKSKTKKLENELEALKDRLLRISAEYENYRKRTDKEKERIYTDACEDVLIKMLPVLDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LERALAVDGTVEDLKKGVEMTVRQFEDALEKLQVEEISTENGFDPELHQAMMVVEQEGAE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
PNQVAQVFQKGYKRGDKVIRHSMVTVTK
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA