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The map label for this gene is slt [H]

Identifier: 18310974

GI number: 18310974

Start: 2281978

End: 2282523

Strand: Reverse

Name: slt [H]

Synonym: CPE1992

Alternate gene names: 18310974

Gene position: 2282523-2281978 (Counterclockwise)

Preceding gene: 18310975

Following gene: 18310973

Centisome position: 75.3

GC content: 29.12

Gene sequence:

>546_bases
ATGAAGAAAATTATAAGGCTAGTTATAATTGTTTTAGCCATATATTTACTATTTTTAGGGAGTGGATATATATTAAAAAA
TGTTATATTTCCATTAAAATATATGAATTATGTAGAGAAATATAGTAAAGAGTATAATTTGGATCCTTATTTTGTCATGG
CAGTTATGAAAGCAGAAAGTAACTTTGACGAAGACGCTAAATCTAATAAGGACGCAAGAGGGCTTATGCAAATTACCACC
TCTACTGGAAAGTGGATAGCTGAAAAACAAGGGATTATTGATTTTGATACCAATTTATTATTAGAGCCAGAAGTTAATAT
AAGGTTTGGATGTTGGTATTTAAATAATCTTCATAAAGAGTTTGGAGATTGGGACCTTGTAATTGCAGCTTACAATGCAG
GAAGAGGACGGGTTCAAGAATGGCTTAATAGTGCTGAACATTCTGATGATGGAAAAAATCTTACTTATATACCATTTAAA
GAAACTGATAAATATGTTAAAAAAGTAAATGTTTATTACAATATATATAGGTCACTTTACTCTTAA

Upstream 100 bases:

>100_bases
TGCTAACATAATAATTGATAATAGCGGTAATTTAATTAAAACAAAGGAACAAATTGATGATTTGTTAGAATTTTTTACTT
TATACCAGTAGGAGAGTTAT

Downstream 100 bases:

>100_bases
GTGACCTATGTATTTTAATATACTATGGATAAAGCTTTGGGAGGGGATGTTTTGAAAAAATGGCTAATAGCTATAGGTGG
TATTTTAATAATTGCTATTT

Product: hypothetical protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKKIIRLVIIVLAIYLLFLGSGYILKNVIFPLKYMNYVEKYSKEYNLDPYFVMAVMKAESNFDEDAKSNKDARGLMQITT
STGKWIAEKQGIIDFDTNLLLEPEVNIRFGCWYLNNLHKEFGDWDLVIAAYNAGRGRVQEWLNSAEHSDDGKNLTYIPFK
ETDKYVKKVNVYYNIYRSLYS

Sequences:

>Translated_181_residues
MKKIIRLVIIVLAIYLLFLGSGYILKNVIFPLKYMNYVEKYSKEYNLDPYFVMAVMKAESNFDEDAKSNKDARGLMQITT
STGKWIAEKQGIIDFDTNLLLEPEVNIRFGCWYLNNLHKEFGDWDLVIAAYNAGRGRVQEWLNSAEHSDDGKNLTYIPFK
ETDKYVKKVNVYYNIYRSLYS
>Mature_181_residues
MKKIIRLVIIVLAIYLLFLGSGYILKNVIFPLKYMNYVEKYSKEYNLDPYFVMAVMKAESNFDEDAKSNKDARGLMQITT
STGKWIAEKQGIIDFDTNLLLEPEVNIRFGCWYLNNLHKEFGDWDLVIAAYNAGRGRVQEWLNSAEHSDDGKNLTYIPFK
ETDKYVKKVNVYYNIYRSLYS

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=151, Percent_Identity=37.7483443708609, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI87082191, Length=167, Percent_Identity=32.3353293413174, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 21190; Mature: 21190

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIRLVIIVLAIYLLFLGSGYILKNVIFPLKYMNYVEKYSKEYNLDPYFVMAVMKAES
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
NFDEDAKSNKDARGLMQITTSTGKWIAEKQGIIDFDTNLLLEPEVNIRFGCWYLNNLHKE
CCCCHHHCCCCCCHHEEEECCCCCCHHHCCCEEEECCCEEECCCCCEEEEHHHHHHHHHH
FGDWDLVIAAYNAGRGRVQEWLNSAEHSDDGKNLTYIPFKETDKYVKKVNVYYNIYRSLY
HCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHC
S
C
>Mature Secondary Structure
MKKIIRLVIIVLAIYLLFLGSGYILKNVIFPLKYMNYVEKYSKEYNLDPYFVMAVMKAES
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
NFDEDAKSNKDARGLMQITTSTGKWIAEKQGIIDFDTNLLLEPEVNIRFGCWYLNNLHKE
CCCCHHHCCCCCCHHEEEECCCCCCHHHCCCEEEECCCEEECCCCCEEEEHHHHHHHHHH
FGDWDLVIAAYNAGRGRVQEWLNSAEHSDDGKNLTYIPFKETDKYVKKVNVYYNIYRSLY
HCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]