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The map label for this gene is potD [H]

Identifier: 18310953

GI number: 18310953

Start: 2259849

End: 2260931

Strand: Direct

Name: potD [H]

Synonym: CPE1971

Alternate gene names: 18310953

Gene position: 2259849-2260931 (Clockwise)

Preceding gene: 18310952

Following gene: 18310955

Centisome position: 74.55

GC content: 30.56

Gene sequence:

>1083_bases
ATGAAATTAAAAAAATTTTTAGCATTAACTTGTTCAACACTTCTTTTATCATCTATTTTTCTTGGTTGTGGGCCAAAAAA
GGATGAGGAAGCTACTCAAGATAAAAACAATAACGTTCTTTATGTTTATAACTGGGGAGATTACATAGATCCAGAACTAC
TTACTAAATTTAAAGAAGAAACTGGAATAGATGTTAAATACGATGTTTATGATACTAATGAAATAATGTATCAAAAACTT
AATAGTGGTAATGTATCTTATGATATAGTAATTCCTTCTGATTACATGATAGAAAAAATGAAAGAAGAAGATATGTTAGC
TAAGATAGATTATTCTAATATTCCTAATTATAAATATATAGGAGAACAATTTAAAAATTTAGCTTATGATCCAACTAATG
AATACTCAGTACCATATATGTGGGGAACAGTTGGAATAATATATAATACAAAGAGAGTTAGTGATCCTGTAGATAGTTGG
AATATCCTTTGGAATCCTAAATATAAGGATCAAGTTATAATGCCAGATAGTGTTAGAGATGCTATGGCTGTTGCAGAAAA
AAAATTAGGATATTCATTAAATACTGAAAACTTAGATCAAATAGAAGCTGCTAAAAAGGAGCTTATGACTCAAAAGAAAG
ATGGATTAATCTTAGCTTACATGGTTGACCAAGTTAAAGATGCCATGGTTGGTGGAGAGGCTTCCCTTGCTGTTGCTTGG
TCTGGAGATGCTGTAACAATGATAGAGAGAAATCCTGATTTAGCTTATGCTATTCCTAAGGAAGGATCAAATAAATGGTT
TGATGCCATAGCAATTCCTAAAAATGCAAAGCATAAAGAAAATGCTGAGAAGTTTATAAACTTCCTTTGCGATCCTGAAA
ATGCTGAACAAAATGTAGAATATATAGGATACTCTACTCCAAACACAGCTGCATATGACCTTTTACCTGAGGATATAAGA
GATGATAAAGTTGCATACCCAGATGAAGAGTCATTAAAAAATTGTGAGGTATTTATAGATTTACCTTCTAAAATACTTAG
AAAATATGATGAAGCTTGGTTAGAAATCAAGTGTGTTTATTAA

Upstream 100 bases:

>100_bases
CCAGAAATAAATGCTCTTTCAACATTAATGTTTATAGTTGTCTTAGCATTACTTCTTATTGCTAATAGAAAACAATCTTT
AGTAAAGGAGGAACGTTAAT

Downstream 100 bases:

>100_bases
AAAAATGCATGTGCAAAAGCACATGCATTTTATATTATCCACAAATTTCTTCATAAAATACTTTTGGGAACCATAGCTTA
ATTTCTTTCTCAGCACTTTC

Product: spermidine/putrescine ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKLKKFLALTCSTLLLSSIFLGCGPKKDEEATQDKNNNVLYVYNWGDYIDPELLTKFKEETGIDVKYDVYDTNEIMYQKL
NSGNVSYDIVIPSDYMIEKMKEEDMLAKIDYSNIPNYKYIGEQFKNLAYDPTNEYSVPYMWGTVGIIYNTKRVSDPVDSW
NILWNPKYKDQVIMPDSVRDAMAVAEKKLGYSLNTENLDQIEAAKKELMTQKKDGLILAYMVDQVKDAMVGGEASLAVAW
SGDAVTMIERNPDLAYAIPKEGSNKWFDAIAIPKNAKHKENAEKFINFLCDPENAEQNVEYIGYSTPNTAAYDLLPEDIR
DDKVAYPDEESLKNCEVFIDLPSKILRKYDEAWLEIKCVY

Sequences:

>Translated_360_residues
MKLKKFLALTCSTLLLSSIFLGCGPKKDEEATQDKNNNVLYVYNWGDYIDPELLTKFKEETGIDVKYDVYDTNEIMYQKL
NSGNVSYDIVIPSDYMIEKMKEEDMLAKIDYSNIPNYKYIGEQFKNLAYDPTNEYSVPYMWGTVGIIYNTKRVSDPVDSW
NILWNPKYKDQVIMPDSVRDAMAVAEKKLGYSLNTENLDQIEAAKKELMTQKKDGLILAYMVDQVKDAMVGGEASLAVAW
SGDAVTMIERNPDLAYAIPKEGSNKWFDAIAIPKNAKHKENAEKFINFLCDPENAEQNVEYIGYSTPNTAAYDLLPEDIR
DDKVAYPDEESLKNCEVFIDLPSKILRKYDEAWLEIKCVY
>Mature_360_residues
MKLKKFLALTCSTLLLSSIFLGCGPKKDEEATQDKNNNVLYVYNWGDYIDPELLTKFKEETGIDVKYDVYDTNEIMYQKL
NSGNVSYDIVIPSDYMIEKMKEEDMLAKIDYSNIPNYKYIGEQFKNLAYDPTNEYSVPYMWGTVGIIYNTKRVSDPVDSW
NILWNPKYKDQVIMPDSVRDAMAVAEKKLGYSLNTENLDQIEAAKKELMTQKKDGLILAYMVDQVKDAMVGGEASLAVAW
SGDAVTMIERNPDLAYAIPKEGSNKWFDAIAIPKNAKHKENAEKFINFLCDPENAEQNVEYIGYSTPNTAAYDLLPEDIR
DDKVAYPDEESLKNCEVFIDLPSKILRKYDEAWLEIKCVY

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=309, Percent_Identity=42.7184466019417, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1787078, Length=339, Percent_Identity=28.9085545722714, Blast_Score=174, Evalue=9e-45,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41248; Mature: 41248

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKFLALTCSTLLLSSIFLGCGPKKDEEATQDKNNNVLYVYNWGDYIDPELLTKFKEE
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
TGIDVKYDVYDTNEIMYQKLNSGNVSYDIVIPSDYMIEKMKEEDMLAKIDYSNIPNYKYI
CCCEEEEEEECCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCEEEECCCCCCCHHHH
GEQFKNLAYDPTNEYSVPYMWGTVGIIYNTKRVSDPVDSWNILWNPKYKDQVIMPDSVRD
HHHHHHCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCEEEECCCCCCEEECCCHHHH
AMAVAEKKLGYSLNTENLDQIEAAKKELMTQKKDGLILAYMVDQVKDAMVGGEASLAVAW
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCEEEEEE
SGDAVTMIERNPDLAYAIPKEGSNKWFDAIAIPKNAKHKENAEKFINFLCDPENAEQNVE
CCCEEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCE
YIGYSTPNTAAYDLLPEDIRDDKVAYPDEESLKNCEVFIDLPSKILRKYDEAWLEIKCVY
EEECCCCCCCEECCCCHHCCCCCCCCCCHHCCCCCEEEEECHHHHHHHHCCCEEEEEEEC
>Mature Secondary Structure
MKLKKFLALTCSTLLLSSIFLGCGPKKDEEATQDKNNNVLYVYNWGDYIDPELLTKFKEE
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
TGIDVKYDVYDTNEIMYQKLNSGNVSYDIVIPSDYMIEKMKEEDMLAKIDYSNIPNYKYI
CCCEEEEEEECCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCEEEECCCCCCCHHHH
GEQFKNLAYDPTNEYSVPYMWGTVGIIYNTKRVSDPVDSWNILWNPKYKDQVIMPDSVRD
HHHHHHCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCEEEECCCCCCEEECCCHHHH
AMAVAEKKLGYSLNTENLDQIEAAKKELMTQKKDGLILAYMVDQVKDAMVGGEASLAVAW
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCEEEEEE
SGDAVTMIERNPDLAYAIPKEGSNKWFDAIAIPKNAKHKENAEKFINFLCDPENAEQNVE
CCCEEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCE
YIGYSTPNTAAYDLLPEDIRDDKVAYPDEESLKNCEVFIDLPSKILRKYDEAWLEIKCVY
EEECCCCCCCEECCCCHHCCCCCCCCCCHHCCCCCEEEEECHHHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]