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The map label for this gene is potB [H]

Identifier: 18310951

GI number: 18310951

Start: 2258208

End: 2259053

Strand: Direct

Name: potB [H]

Synonym: CPE1969

Alternate gene names: 18310951

Gene position: 2258208-2259053 (Clockwise)

Preceding gene: 18310950

Following gene: 18310952

Centisome position: 74.49

GC content: 29.08

Gene sequence:

>846_bases
ATGAAAAATAGAAATAAAAATTTATCATTTGCAGCTTATCCATACGTTGTATGGAGTGCTATATTCATAGTTATCCCTTT
ACTTTTAATAGTTTTCTTTAGTTTTACAAGTAAAGTTGATGGAAGATTTGTTTTCTCTTTTGAAAACTTCCAAAGATTAT
TTGAGCCAATATATTTTACTGTTTTTATACGTTCTATATGGCTTGCTGTTTTATCAACAGTTTCATGTTTAATTTTAGGT
TATCCAATAGCTTATATAATTTCTAAATTACCTATAAAAAGAAGAAATATGTTAATACTTTTATTCATATTACCAATGTG
GATGAACTTCTTACTTAGAACATATGCGTGGATGGCTATACTTGGTAGAGATGGGCTTATTAATACTCTTTTAGGTTATA
TAGGAATAGGTCCAATTAAAATGCTATATACTGACGGAGCAATCTTACTTGGAATGGTATATAACTTCTTACCATTTATG
GTTATACCAATATATACTGTACTTATAAAAATTGATAAAAACTTAGTTAATGCAGCTTATGACTTAGGTGCAAATAAAGC
TCAAGCTTTTAGAAAAATTATTTTACCACTTAGTATTCCTGGAATAATCTCTGGAATAACTATGGTATTTATGCCAGCTG
TTTCAAACTTCGTAATCCCAAGCCTTCTAGGTGGTGGAAAATACATGTTAGTTGGTAATCTTATAGAACAACAATTTACT
ACTATCGGTAACTGGAACTTCGGTTCTGCTTTATCAATCTTTATGATGATATTAATATTAATTTCAATGGCCTTTATGTC
TAAATATGAAAAGAACGGAAAAGAAGGAGGTAAACAGCTATGGTAA

Upstream 100 bases:

>100_bases
TATTACACAATACAAAACATGCTGAAATAGGATCTAAAATAGGATTATCTCTTGATCCAGAAAGCATACACATAATGAAA
AAAGAGAGTGATGTTTAAAG

Downstream 100 bases:

>100_bases
AAAAGTTAGGTGATTTTATTTCTAAATTTTATTTAGCCTTAGTATTTATATTTTTATATGCACCAATAGTAACACTTATG
ATATTTTCATTTAACAAAAC

Product: spermidine/putrescine ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKNRNKNLSFAAYPYVVWSAIFIVIPLLLIVFFSFTSKVDGRFVFSFENFQRLFEPIYFTVFIRSIWLAVLSTVSCLILG
YPIAYIISKLPIKRRNMLILLFILPMWMNFLLRTYAWMAILGRDGLINTLLGYIGIGPIKMLYTDGAILLGMVYNFLPFM
VIPIYTVLIKIDKNLVNAAYDLGANKAQAFRKIILPLSIPGIISGITMVFMPAVSNFVIPSLLGGGKYMLVGNLIEQQFT
TIGNWNFGSALSIFMMILILISMAFMSKYEKNGKEGGKQLW

Sequences:

>Translated_281_residues
MKNRNKNLSFAAYPYVVWSAIFIVIPLLLIVFFSFTSKVDGRFVFSFENFQRLFEPIYFTVFIRSIWLAVLSTVSCLILG
YPIAYIISKLPIKRRNMLILLFILPMWMNFLLRTYAWMAILGRDGLINTLLGYIGIGPIKMLYTDGAILLGMVYNFLPFM
VIPIYTVLIKIDKNLVNAAYDLGANKAQAFRKIILPLSIPGIISGITMVFMPAVSNFVIPSLLGGGKYMLVGNLIEQQFT
TIGNWNFGSALSIFMMILILISMAFMSKYEKNGKEGGKQLW
>Mature_281_residues
MKNRNKNLSFAAYPYVVWSAIFIVIPLLLIVFFSFTSKVDGRFVFSFENFQRLFEPIYFTVFIRSIWLAVLSTVSCLILG
YPIAYIISKLPIKRRNMLILLFILPMWMNFLLRTYAWMAILGRDGLINTLLGYIGIGPIKMLYTDGAILLGMVYNFLPFM
VIPIYTVLIKIDKNLVNAAYDLGANKAQAFRKIILPLSIPGIISGITMVFMPAVSNFVIPSLLGGGKYMLVGNLIEQQFT
TIGNWNFGSALSIFMMILILISMAFMSKYEKNGKEGGKQLW

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=247, Percent_Identity=42.5101214574899, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1787080, Length=244, Percent_Identity=40.9836065573771, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787713, Length=252, Percent_Identity=30.1587301587302, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31877; Mature: 31877

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]