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The map label for this gene is vhb [H]

Identifier: 18310935

GI number: 18310935

Start: 2241885

End: 2242319

Strand: Reverse

Name: vhb [H]

Synonym: CPE1953

Alternate gene names: 18310935

Gene position: 2242319-2241885 (Counterclockwise)

Preceding gene: 18310936

Following gene: 18310934

Centisome position: 73.97

GC content: 28.97

Gene sequence:

>435_bases
ATGTTAGATCAAAAGACAATAGATATAATAAAAAGTACAGTACCAGTTTTAAAATCCAATGGATTAGAAATAACAAAAAC
TTTCTATAAAAATATGTTTGAACAAAATCCTGAAGTAAAACCTTTGTTCAATATGAATAAACAAGAATCTGAAGAGCAAC
CAAAAGCTTTAGCAATGGCAATATTAGCAGTTGCTCAAAATATAGATAACTTAGAAGCTATAAAACCTGTAGTTAATAGA
ATAGGAGTTATACACTGTAATGCAAAAGTTCAACCAGAACATTATCCAATAGTAGGTAAACACTTATTAGGAGCAATAAA
AGAAGTTTTAGGTGATGGTGCAACTGAAGATATTATCAATGCGTGGGCTAAAACATATGGAGTTATAGCTGAAGTATTTA
TAAATAATGAAAAAGAGATGTATGCTTCAAGATAG

Upstream 100 bases:

>100_bases
AAAAAAATATTTAATGTTTTTAACTTGAAAAAATTTTAAATTATTGTATAATATAATCAATAATAATTATTAATTAAAAA
TAAATATTACGGAGGAGATT

Downstream 100 bases:

>100_bases
AATAATATATAAGATGAGCTGTTAAATCAGCTCATCTTTTTTATTTTATTTTTAATGAAATAAAAAATAAATTTTAATAT
AGTTATTTCTTAGTTTCTCT

Product: hemoglobin

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Soluble cytochrome O [H]

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MLDQKTIDIIKSTVPVLKSNGLEITKTFYKNMFEQNPEVKPLFNMNKQESEEQPKALAMAILAVAQNIDNLEAIKPVVNR
IGVIHCNAKVQPEHYPIVGKHLLGAIKEVLGDGATEDIINAWAKTYGVIAEVFINNEKEMYASR

Sequences:

>Translated_144_residues
MLDQKTIDIIKSTVPVLKSNGLEITKTFYKNMFEQNPEVKPLFNMNKQESEEQPKALAMAILAVAQNIDNLEAIKPVVNR
IGVIHCNAKVQPEHYPIVGKHLLGAIKEVLGDGATEDIINAWAKTYGVIAEVFINNEKEMYASR
>Mature_144_residues
MLDQKTIDIIKSTVPVLKSNGLEITKTFYKNMFEQNPEVKPLFNMNKQESEEQPKALAMAILAVAQNIDNLEAIKPVVNR
IGVIHCNAKVQPEHYPIVGKHLLGAIKEVLGDGATEDIINAWAKTYGVIAEVFINNEKEMYASR

Specific function: This protein functions as a terminal oxidase [H]

COG id: COG1017

COG function: function code C; Hemoglobin-like flavoprotein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the globin family [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=141, Percent_Identity=45.3900709219858, Blast_Score=134, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321673, Length=141, Percent_Identity=53.1914893617021, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971 [H]

Pfam domain/function: PF00042 Globin [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 16092; Mature: 16092

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQKTIDIIKSTVPVLKSNGLEITKTFYKNMFEQNPEVKPLFNMNKQESEEQPKALAMA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
ILAVAQNIDNLEAIKPVVNRIGVIHCNAKVQPEHYPIVGKHLLGAIKEVLGDGATEDIIN
HHHHHHCCCHHHHHHHHHHHCCEEECCCEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
AWAKTYGVIAEVFINNEKEMYASR
HHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MLDQKTIDIIKSTVPVLKSNGLEITKTFYKNMFEQNPEVKPLFNMNKQESEEQPKALAMA
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
ILAVAQNIDNLEAIKPVVNRIGVIHCNAKVQPEHYPIVGKHLLGAIKEVLGDGATEDIIN
HHHHHHCCCHHHHHHHHHHHCCEEECCCEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
AWAKTYGVIAEVFINNEKEMYASR
HHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3067078; 3736670; 2685332; 2681149; 9115439 [H]