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The map label for this gene is folD [H]

Identifier: 18310805

GI number: 18310805

Start: 2101416

End: 2102249

Strand: Reverse

Name: folD [H]

Synonym: CPE1823

Alternate gene names: 18310805

Gene position: 2102249-2101416 (Counterclockwise)

Preceding gene: 18310806

Following gene: 18310804

Centisome position: 69.35

GC content: 31.53

Gene sequence:

>834_bases
ATGGATAAAATTTTAAGCGGAAAAACAGTGGCTATAGACATAAAGGGGCAAATTAAGAGTTATACAGAGGAATTAAAAGC
TTCTGGAAAGTCTTTAAAAATATCGTCTATACTAGTTGGGGATGATGGAGGCTCAGTATATTACCAAAACTTCCAAGAAA
AATTAGCTAATAATTTAGGAATAGACTTTGAGAAGATAAAACTAGATGAAAGTATTTCAGAAGAAAATCTGAAACTTAAG
ATAGAAGAGTTAAATAAAGATGACAGTGTAAATGGAATAATGCTTTTATTACCTTTACCAAAGCATATTGATGAGAGGGC
AGTTACAAATTTAATAGATGCAGATAAAGACTTAGATTGTCTTTCTGAGGTAAGTGTAGGTAGATTCTATAAGGGTGAGA
AGTGTTTTATGCCATGCACACCAAACAGTGTAATAACTCTTTTAAAAGCTTATAACATTGAAATTGAGGGTAAAGAAGTT
GTTATTATTGGAAGAAGTAATATAGTTGGTAAACCTCTTTTTCAAATGTTTTTAAATGAAAATGCTACAGTAACAGTATG
TCATTCAAGAACTAAGAACTTAAAAGAAGTTTGTAAGAGAGCTGACATATTAGTAGTAGCTATTGGAAGGGCTAATTTCA
TAGATTCTTCTTATGTTAGAGAAGGAGCGGTTGTTATTGATGTAGGAACTAGTGAGGTTAATGGTAAAATAACGGGAGAT
GTTAATTTTGATGATGTTTATGACAAAGCATCATTAATTACACCAGTTCCAGGAGGCGTAGGTTCTTTAACTACGACTCT
TCTTTTAAAAAATGTTTGTAAGGAGTTAGATTAG

Upstream 100 bases:

>100_bases
AGTTTCATTTATAAATGGAGTTTTAGATAAAGTATATAAGAATATGCAATAGGGGCTAAATTTATTTTTACTAGAATTAT
AAGATTTGGAGGGGCAGGTA

Downstream 100 bases:

>100_bases
AATGAAACTAAAGACATTATCTGTTGGTGAAGTTAATAACTACGTTAAAAAGTTAGTTGAAAATGACTTTATATTAAAGA
ATCTTAATGTCAAAGGTGAA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MDKILSGKTVAIDIKGQIKSYTEELKASGKSLKISSILVGDDGGSVYYQNFQEKLANNLGIDFEKIKLDESISEENLKLK
IEELNKDDSVNGIMLLLPLPKHIDERAVTNLIDADKDLDCLSEVSVGRFYKGEKCFMPCTPNSVITLLKAYNIEIEGKEV
VIIGRSNIVGKPLFQMFLNENATVTVCHSRTKNLKEVCKRADILVVAIGRANFIDSSYVREGAVVIDVGTSEVNGKITGD
VNFDDVYDKASLITPVPGGVGSLTTTLLLKNVCKELD

Sequences:

>Translated_277_residues
MDKILSGKTVAIDIKGQIKSYTEELKASGKSLKISSILVGDDGGSVYYQNFQEKLANNLGIDFEKIKLDESISEENLKLK
IEELNKDDSVNGIMLLLPLPKHIDERAVTNLIDADKDLDCLSEVSVGRFYKGEKCFMPCTPNSVITLLKAYNIEIEGKEV
VIIGRSNIVGKPLFQMFLNENATVTVCHSRTKNLKEVCKRADILVVAIGRANFIDSSYVREGAVVIDVGTSEVNGKITGD
VNFDDVYDKASLITPVPGGVGSLTTTLLLKNVCKELD
>Mature_277_residues
MDKILSGKTVAIDIKGQIKSYTEELKASGKSLKISSILVGDDGGSVYYQNFQEKLANNLGIDFEKIKLDESISEENLKLK
IEELNKDDSVNGIMLLLPLPKHIDERAVTNLIDADKDLDCLSEVSVGRFYKGEKCFMPCTPNSVITLLKAYNIEIEGKEV
VIIGRSNIVGKPLFQMFLNENATVTVCHSRTKNLKEVCKRADILVVAIGRANFIDSSYVREGAVVIDVGTSEVNGKITGD
VNFDDVYDKASLITPVPGGVGSLTTTLLLKNVCKELD

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=282, Percent_Identity=37.2340425531915, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI94721354, Length=285, Percent_Identity=40, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI222418558, Length=285, Percent_Identity=37.8947368421053, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI36796743, Length=206, Percent_Identity=23.3009708737864, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1786741, Length=274, Percent_Identity=39.4160583941606, Blast_Score=204, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI17568735, Length=271, Percent_Identity=40.590405904059, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6319558, Length=280, Percent_Identity=40, Blast_Score=207, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321643, Length=288, Percent_Identity=36.8055555555556, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322933, Length=319, Percent_Identity=26.6457680250784, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=39.0243902439024, Blast_Score=191, Evalue=6e-49,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=39.0243902439024, Blast_Score=191, Evalue=6e-49,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=36.426116838488, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17136816, Length=291, Percent_Identity=36.426116838488, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=39.0243902439024, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=39.0243902439024, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30337; Mature: 30337

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKILSGKTVAIDIKGQIKSYTEELKASGKSLKISSILVGDDGGSVYYQNFQEKLANNLG
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCC
IDFEKIKLDESISEENLKLKIEELNKDDSVNGIMLLLPLPKHIDERAVTNLIDADKDLDC
CCCEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHH
LSEVSVGRFYKGEKCFMPCTPNSVITLLKAYNIEIEGKEVVIIGRSNIVGKPLFQMFLNE
HHHCCCCCEECCCEEEECCCCHHHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHCC
NATVTVCHSRTKNLKEVCKRADILVVAIGRANFIDSSYVREGAVVIDVGTSEVNGKITGD
CCEEEEECCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCEEEEECCCCCCCEEEEC
VNFDDVYDKASLITPVPGGVGSLTTTLLLKNVCKELD
CCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKILSGKTVAIDIKGQIKSYTEELKASGKSLKISSILVGDDGGSVYYQNFQEKLANNLG
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCC
IDFEKIKLDESISEENLKLKIEELNKDDSVNGIMLLLPLPKHIDERAVTNLIDADKDLDC
CCCEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHH
LSEVSVGRFYKGEKCFMPCTPNSVITLLKAYNIEIEGKEVVIIGRSNIVGKPLFQMFLNE
HHHCCCCCEECCCEEEECCCCHHHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHCC
NATVTVCHSRTKNLKEVCKRADILVVAIGRANFIDSSYVREGAVVIDVGTSEVNGKITGD
CCEEEEECCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCEEEEECCCCCCCEEEEC
VNFDDVYDKASLITPVPGGVGSLTTTLLLKNVCKELD
CCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA