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The map label for this gene is dacF [H]

Identifier: 18310788

GI number: 18310788

Start: 2085161

End: 2086369

Strand: Reverse

Name: dacF [H]

Synonym: CPE1806

Alternate gene names: 18310788

Gene position: 2086369-2085161 (Counterclockwise)

Preceding gene: 18310789

Following gene: 18310787

Centisome position: 68.82

GC content: 31.51

Gene sequence:

>1209_bases
ATGAAAAAATACATAAGAAAAATAGCAGCTTTTGCGATATTTTTATCTTTATTTAGCATTGTTTCTTCTGTTCCTGTAAA
AGCTGAAGAGGCAATAGAAGTAGAAGCAAGATCAGCTCTTCTTATGGAAGCTAGTACAGGAAAAATAATTTATGAAAAAA
ATGCTAATGAAAAATTTGCACCTGCTTCAGTAACAAAGATTATGACAATGTTACTTGCCATGGAAAAAGTAGATAGAGGA
GAAATAAAACTTTCAGATCAAATCACAGTAAGTGAGACTGCTAAGAAAATGGGTGGTAGTACTATGCTCCTAGATGTTGG
AGAAGTAAGAACTGTTGAGGAGATTATAAAAGGTATAGGAATAGCTTCAGGTAATGATGCCGCTGTAGCTATGGCTGAAT
ATTTAGGTGGAAGTGAAAGTGGTTTTGTTGAGATGATGAACAAAAGAGCTAAGGAGCTTGGAATGAAAAACACTACATTT
AAAAACTGCACAGGGCTTCCTATAGAAGGACATTTATCTACTGCATATGATATTTCTATTATGTCTAGGGAATTATTAAA
ACATCCTACTATATTAAAATATACAGGAACTTATATGGAAACTATATCAGAGGGAAGAAAATCTCCTATTGAACTTGTTA
ACCATAATAAATTAGTTAGATTCTTTAAGGGATGTGATGGTCTTAAAACTGGATTTACTAATGACGCTAAATACTGTATA
TCAGCTACAGCAACTAGGGACGGAGTTAGAATGCTTTCAGTAATAATGGGAGCTCCAACATTTAAAATAAGAAATAGAGA
TGCTAGTATGCTTATGAATTATGGATTTTCTAAGTTTGAAAGCAAGCAAGTAGCTACAAAAGATGGTGAGGTAGAACAAA
TTCCTATGAGTCAAACAGGAGATAAGTTTATCATAGGAAAAGCTAAGGATGATTTAACTTTAGTTTGTCCAAAGGGTATG
AGTGACAAAATAGAGAAAAAAGTTGTTTTAGATGAAAGTAAGAAAAAAATTAAAGAATTTGATACTATAGGATATTGTGA
ATATTATCTAGATGGTGAACTTCTAGGTAAAGTTGAATTATATTCAGATAGAGATATGAAAAAAGGCGGAATTATTGATA
GTGTTAAATTCAAGGTTAAGAATTTCTTTAATGGTGAAGAGGAAGATAAAGTAGAAGAAAAAACAGAAGAAAAGAGTAAT
GAAAAATAA

Upstream 100 bases:

>100_bases
TAAAGTTATCCTATACAAATTAAAGTATAGGATAACTTTTTCTTTTTTGCTTATAATTTATTTCTTTTGGGAGAAAATAA
GCAAGAAAGGAGGAGATATT

Downstream 100 bases:

>100_bases
AGTATTAATTAGACATTTTGATTGTATTCATTAAAAATAAATACCTATAAGTTGAAAGAAGAACAACTAGCATATAGTCT
CCTTATGCTAGTTGTTTTTA

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKKYIRKIAAFAIFLSLFSIVSSVPVKAEEAIEVEARSALLMEASTGKIIYEKNANEKFAPASVTKIMTMLLAMEKVDRG
EIKLSDQITVSETAKKMGGSTMLLDVGEVRTVEEIIKGIGIASGNDAAVAMAEYLGGSESGFVEMMNKRAKELGMKNTTF
KNCTGLPIEGHLSTAYDISIMSRELLKHPTILKYTGTYMETISEGRKSPIELVNHNKLVRFFKGCDGLKTGFTNDAKYCI
SATATRDGVRMLSVIMGAPTFKIRNRDASMLMNYGFSKFESKQVATKDGEVEQIPMSQTGDKFIIGKAKDDLTLVCPKGM
SDKIEKKVVLDESKKKIKEFDTIGYCEYYLDGELLGKVELYSDRDMKKGGIIDSVKFKVKNFFNGEEEDKVEEKTEEKSN
EK

Sequences:

>Translated_402_residues
MKKYIRKIAAFAIFLSLFSIVSSVPVKAEEAIEVEARSALLMEASTGKIIYEKNANEKFAPASVTKIMTMLLAMEKVDRG
EIKLSDQITVSETAKKMGGSTMLLDVGEVRTVEEIIKGIGIASGNDAAVAMAEYLGGSESGFVEMMNKRAKELGMKNTTF
KNCTGLPIEGHLSTAYDISIMSRELLKHPTILKYTGTYMETISEGRKSPIELVNHNKLVRFFKGCDGLKTGFTNDAKYCI
SATATRDGVRMLSVIMGAPTFKIRNRDASMLMNYGFSKFESKQVATKDGEVEQIPMSQTGDKFIIGKAKDDLTLVCPKGM
SDKIEKKVVLDESKKKIKEFDTIGYCEYYLDGELLGKVELYSDRDMKKGGIIDSVKFKVKNFFNGEEEDKVEEKTEEKSN
EK
>Mature_402_residues
MKKYIRKIAAFAIFLSLFSIVSSVPVKAEEAIEVEARSALLMEASTGKIIYEKNANEKFAPASVTKIMTMLLAMEKVDRG
EIKLSDQITVSETAKKMGGSTMLLDVGEVRTVEEIIKGIGIASGNDAAVAMAEYLGGSESGFVEMMNKRAKELGMKNTTF
KNCTGLPIEGHLSTAYDISIMSRELLKHPTILKYTGTYMETISEGRKSPIELVNHNKLVRFFKGCDGLKTGFTNDAKYCI
SATATRDGVRMLSVIMGAPTFKIRNRDASMLMNYGFSKFESKQVATKDGEVEQIPMSQTGDKFIIGKAKDDLTLVCPKGM
SDKIEKKVVLDESKKKIKEFDTIGYCEYYLDGELLGKVELYSDRDMKKGGIIDSVKFKVKNFFNGEEEDKVEEKTEEKSN
EK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=401, Percent_Identity=32.1695760598504, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786851, Length=389, Percent_Identity=30.0771208226221, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87082022, Length=364, Percent_Identity=30.4945054945055, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082054, Length=211, Percent_Identity=29.3838862559242, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44632; Mature: 44632

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYIRKIAAFAIFLSLFSIVSSVPVKAEEAIEVEARSALLMEASTGKIIYEKNANEKFA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEECCCCCEEEECCCCCCCC
PASVTKIMTMLLAMEKVDRGEIKLSDQITVSETAKKMGGSTMLLDVGEVRTVEEIIKGIG
CHHHHHHHHHHHHHHHCCCCCEEECCCEEHHHHHHHCCCCEEEEECCHHHHHHHHHHHCC
IASGNDAAVAMAEYLGGSESGFVEMMNKRAKELGMKNTTFKNCTGLPIEGHLSTAYDISI
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MSRELLKHPTILKYTGTYMETISEGRKSPIELVNHNKLVRFFKGCDGLKTGFTNDAKYCI
HHHHHHCCCCEEEECCHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEE
SATATRDGVRMLSVIMGAPTFKIRNRDASMLMNYGFSKFESKQVATKDGEVEQIPMSQTG
EECCCHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHCCCCCCCCCCCEEECCCCCCC
DKFIIGKAKDDLTLVCPKGMSDKIEKKVVLDESKKKIKEFDTIGYCEYYLDGELLGKVEL
CEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEE
YSDRDMKKGGIIDSVKFKVKNFFNGEEEDKVEEKTEEKSNEK
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKYIRKIAAFAIFLSLFSIVSSVPVKAEEAIEVEARSALLMEASTGKIIYEKNANEKFA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEECCCCCEEEECCCCCCCC
PASVTKIMTMLLAMEKVDRGEIKLSDQITVSETAKKMGGSTMLLDVGEVRTVEEIIKGIG
CHHHHHHHHHHHHHHHCCCCCEEECCCEEHHHHHHHCCCCEEEEECCHHHHHHHHHHHCC
IASGNDAAVAMAEYLGGSESGFVEMMNKRAKELGMKNTTFKNCTGLPIEGHLSTAYDISI
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MSRELLKHPTILKYTGTYMETISEGRKSPIELVNHNKLVRFFKGCDGLKTGFTNDAKYCI
HHHHHHCCCCEEEECCHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEE
SATATRDGVRMLSVIMGAPTFKIRNRDASMLMNYGFSKFESKQVATKDGEVEQIPMSQTG
EECCCHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHCCCCCCCCCCCEEECCCCCCC
DKFIIGKAKDDLTLVCPKGMSDKIEKKVVLDESKKKIKEFDTIGYCEYYLDGELLGKVEL
CEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEE
YSDRDMKKGGIIDSVKFKVKNFFNGEEEDKVEEKTEEKSNEK
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]