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The map label for this gene is pleD [H]

Identifier: 18310770

GI number: 18310770

Start: 2067478

End: 2068377

Strand: Reverse

Name: pleD [H]

Synonym: CPE1788

Alternate gene names: 18310770

Gene position: 2068377-2067478 (Counterclockwise)

Preceding gene: 18310771

Following gene: 18310769

Centisome position: 68.23

GC content: 26.0

Gene sequence:

>900_bases
TTGGAAGTTTTACTAGATGTAAAAGAAAGAATGTCTATATTAAAGAACTTTTATGATGAAGTTAGAGTGGTAGATCCTAT
AAATAATATAGTATTTTCAGATGACTTTAACCTAAAAATCAATAATAAATTTAATGAAAAAGATTGTTCCCTAAGCAATT
GTTATGATGTGTGGGGAAAAAAATCAATGTGCAGTACTTGCATATCAAAAAAAGCTTATTTAAAAAATGATACTTTTATA
AAGTTAGAATATAATAAAAATCATGTGTTTTTAGTTATAGCTTCCCCAATTGAATTTAATGAAAATAAATATGTTGTTGA
AATATTAAAAGATATAACTAATAATGGCAGTATAGTAGATGGTAGTGGAGAAGGTAATTATTTAGACTTAACAATGAAAA
AGATGGGAGAAAATCTTATTAAGGATTATTTAACTGGAGTCTATAATAAAAGGTATATAGATCAGAGATTTTCAAAGGAA
GCTCATAGAAACTTAAAAGAATCTATACCTACAACTGTAATTATGACTGATATAGATTCCTTTAAAAAAGTAAATGATAC
CTATGGTCATTTAGTAGGTGACAAAATTTTAAGGGATTTTGCAAAGGTATTAAATAATAATATCAGAGAAAATAGTGATT
GGATTGGTAGATATGGAGGAGAAGAATTCATAATTGTTTTAAATAACACAAATTTAAAAAATGGAGTGAAGGTTGCTGAG
AAGCTTAGAAAAATCATAGAAAAAATGAGTTTTGACTATGGTGATTTAAGTTTAAAAATAACTTCTAGCTTCGGAGTTTG
TGAAGTTTCAGAAAAAGAAGATCCCTTTGATACCATAAAAAATGCTGATGAAAAATTATATATGGCAAAAATGACAGGAA
GAAATAAAACTGTATTTTAG

Upstream 100 bases:

>100_bases
GAGAGGTACAAATTTAAGAATAATTAGTTTTAGATATTCCTTTAATATGTAGTAAGGTGTAGAAAGAAATTAAAAGTTCT
AAGGACTGGAGGAAAGAATA

Downstream 100 bases:

>100_bases
TATTATAATAAATTTTAGAATTAAATTAAGATTTACTTTAGGAAATTCATTAACTATAAAAGTATAAGAAGGGAGGAAAA
TTATCAAATTTCATATTAAT

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MEVLLDVKERMSILKNFYDEVRVVDPINNIVFSDDFNLKINNKFNEKDCSLSNCYDVWGKKSMCSTCISKKAYLKNDTFI
KLEYNKNHVFLVIASPIEFNENKYVVEILKDITNNGSIVDGSGEGNYLDLTMKKMGENLIKDYLTGVYNKRYIDQRFSKE
AHRNLKESIPTTVIMTDIDSFKKVNDTYGHLVGDKILRDFAKVLNNNIRENSDWIGRYGGEEFIIVLNNTNLKNGVKVAE
KLRKIIEKMSFDYGDLSLKITSSFGVCEVSEKEDPFDTIKNADEKLYMAKMTGRNKTVF

Sequences:

>Translated_299_residues
MEVLLDVKERMSILKNFYDEVRVVDPINNIVFSDDFNLKINNKFNEKDCSLSNCYDVWGKKSMCSTCISKKAYLKNDTFI
KLEYNKNHVFLVIASPIEFNENKYVVEILKDITNNGSIVDGSGEGNYLDLTMKKMGENLIKDYLTGVYNKRYIDQRFSKE
AHRNLKESIPTTVIMTDIDSFKKVNDTYGHLVGDKILRDFAKVLNNNIRENSDWIGRYGGEEFIIVLNNTNLKNGVKVAE
KLRKIIEKMSFDYGDLSLKITSSFGVCEVSEKEDPFDTIKNADEKLYMAKMTGRNKTVF
>Mature_299_residues
MEVLLDVKERMSILKNFYDEVRVVDPINNIVFSDDFNLKINNKFNEKDCSLSNCYDVWGKKSMCSTCISKKAYLKNDTFI
KLEYNKNHVFLVIASPIEFNENKYVVEILKDITNNGSIVDGSGEGNYLDLTMKKMGENLIKDYLTGVYNKRYIDQRFSKE
AHRNLKESIPTTVIMTDIDSFKKVNDTYGHLVGDKILRDFAKVLNNNIRENSDWIGRYGGEEFIIVLNNTNLKNGVKVAE
KLRKIIEKMSFDYGDLSLKITSSFGVCEVSEKEDPFDTIKNADEKLYMAKMTGRNKTVF

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=38.125, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=35.8490566037736, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=34.7826086956522, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082007, Length=158, Percent_Identity=34.8101265822785, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=33.9181286549708, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=31.4465408805031, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081881, Length=205, Percent_Identity=29.7560975609756, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787541, Length=245, Percent_Identity=26.1224489795918, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=27.8145695364238, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=28.9308176100629, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788381, Length=198, Percent_Identity=25.7575757575758, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=29.0123456790123, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=33.9393939393939, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34481; Mature: 34481

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVLLDVKERMSILKNFYDEVRVVDPINNIVFSDDFNLKINNKFNEKDCSLSNCYDVWGK
CCHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHCC
KSMCSTCISKKAYLKNDTFIKLEYNKNHVFLVIASPIEFNENKYVVEILKDITNNGSIVD
HHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCEEECCCHHHHHHHHHHCCCCCEEE
GSGEGNYLDLTMKKMGENLIKDYLTGVYNKRYIDQRFSKEAHRNLKESIPTTVIMTDIDS
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHH
FKKVNDTYGHLVGDKILRDFAKVLNNNIRENSDWIGRYGGEEFIIVLNNTNLKNGVKVAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEECCCCCHHHHHHH
KLRKIIEKMSFDYGDLSLKITSSFGVCEVSEKEDPFDTIKNADEKLYMAKMTGRNKTVF
HHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCHHHHCCCCHHEEEEEECCCCCCCC
>Mature Secondary Structure
MEVLLDVKERMSILKNFYDEVRVVDPINNIVFSDDFNLKINNKFNEKDCSLSNCYDVWGK
CCHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHCC
KSMCSTCISKKAYLKNDTFIKLEYNKNHVFLVIASPIEFNENKYVVEILKDITNNGSIVD
HHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCEEECCCHHHHHHHHHHCCCCCEEE
GSGEGNYLDLTMKKMGENLIKDYLTGVYNKRYIDQRFSKEAHRNLKESIPTTVIMTDIDS
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHH
FKKVNDTYGHLVGDKILRDFAKVLNNNIRENSDWIGRYGGEEFIIVLNNTNLKNGVKVAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEECCCCCHHHHHHH
KLRKIIEKMSFDYGDLSLKITSSFGVCEVSEKEDPFDTIKNADEKLYMAKMTGRNKTVF
HHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCHHHHCCCCHHEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA