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The map label for this gene is sigE [H]

Identifier: 18310744

GI number: 18310744

Start: 2040448

End: 2041155

Strand: Reverse

Name: sigE [H]

Synonym: CPE1762

Alternate gene names: 18310744

Gene position: 2041155-2040448 (Counterclockwise)

Preceding gene: 18310745

Following gene: 18310743

Centisome position: 67.33

GC content: 28.53

Gene sequence:

>708_bases
ATGTTAAATATAAGACTTTTGCTTAATAAGTTATTTACTAAATTAGATATTATGGTAGAACCAATTTATTATGTTGGGGG
GAATGATGTTCTTCCAGCACCTCTTAGTAAAGAAGAGGAAGAGGAGTTAGTTAACAAATTAGGGGAGAATGATGAAGCTA
TTAGAAGTATTCTTATTGAGAGAAATTTAAGACTAGTTGTATATATAGCTAGAAAATTTGAGAATACTGGTGTAGGCGTT
GAGGATTTAATTTCTGTAGGAACAATTGGACTTATAAAGGCAGTAAATACATTTAATCCAGAAAAAAAGATAAAGCTTGC
AACCTATGCATCAAGATGTATAGAAAATGAAATTCTTATGTATTTAAGGAGAAATAGCAAGGTTAAAGCTGAGATATCAT
TTTATGAGCCCTTAAATATAGATTGGGATGGAAATGAACTTTTACTTTCAGATATACTTGGCACAGATGATGATATTGTT
TACAATCTGATAGAAGATGAAGTTGACAAGGAATTATTATTTACTGCAATGAAGAATCTTTCAAATAGAGAAAAAGAAAT
TGTTGAATTAAGATTTGGACTATGTGGATATAAAGAAAAAACTCAAAAGGAAGTAGCTGATATGCTAGGAATATCTCAAT
CTTATATTTCAAGGTTAGAGAAGAAAATTATAAAAAGATTAAAGAAAGAAATTAACAAAATGATTTAA

Upstream 100 bases:

>100_bases
ACAAGAGATGCAATCTTATGTTTTTGTGATAATAAATTAAGTAAAGAGGGAGAATATGAAGCTTTATTGTCTAGAGGAAT
TATATAGGGGTGATTAGGTT

Downstream 100 bases:

>100_bases
GAGTATAAAATTAACCTCTACAGGTAATAATTAAAATGCGGAAGGGAATTACTTCGAAGGGGTTGATTTCAATTATGATG
ATAAATAAAGTAGAAATATG

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLNIRLLLNKLFTKLDIMVEPIYYVGGNDVLPAPLSKEEEEELVNKLGENDEAIRSILIERNLRLVVYIARKFENTGVGV
EDLISVGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDDDIV
YNLIEDEVDKELLFTAMKNLSNREKEIVELRFGLCGYKEKTQKEVADMLGISQSYISRLEKKIIKRLKKEINKMI

Sequences:

>Translated_235_residues
MLNIRLLLNKLFTKLDIMVEPIYYVGGNDVLPAPLSKEEEEELVNKLGENDEAIRSILIERNLRLVVYIARKFENTGVGV
EDLISVGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDDDIV
YNLIEDEVDKELLFTAMKNLSNREKEIVELRFGLCGYKEKTQKEVADMLGISQSYISRLEKKIIKRLKKEINKMI
>Mature_235_residues
MLNIRLLLNKLFTKLDIMVEPIYYVGGNDVLPAPLSKEEEEELVNKLGENDEAIRSILIERNLRLVVYIARKFENTGVGV
EDLISVGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDDDIV
YNLIEDEVDKELLFTAMKNLSNREKEIVELRFGLCGYKEKTQKEVADMLGISQSYISRLEKKIIKRLKKEINKMI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=248, Percent_Identity=27.8225806451613, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789871, Length=107, Percent_Identity=38.3177570093458, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27064; Mature: 27064

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIRLLLNKLFTKLDIMVEPIYYVGGNDVLPAPLSKEEEEELVNKLGENDEAIRSILIE
CCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
RNLRLVVYIARKFENTGVGVEDLISVGTIGLIKAVNTFNPEKKIKLATYASRCIENEILM
CCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDDDIVYNLIEDEVDKELLFTAMKNL
HHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SNREKEIVELRFGLCGYKEKTQKEVADMLGISQSYISRLEKKIIKRLKKEINKMI
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNIRLLLNKLFTKLDIMVEPIYYVGGNDVLPAPLSKEEEEELVNKLGENDEAIRSILIE
CCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
RNLRLVVYIARKFENTGVGVEDLISVGTIGLIKAVNTFNPEKKIKLATYASRCIENEILM
CCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDDDIVYNLIEDEVDKELLFTAMKNL
HHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SNREKEIVELRFGLCGYKEKTQKEVADMLGISQSYISRLEKKIIKRLKKEINKMI
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883192; 11466286; 7961408 [H]