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The map label for this gene is sigG [H]

Identifier: 18310743

GI number: 18310743

Start: 2039600

End: 2040412

Strand: Reverse

Name: sigG [H]

Synonym: CPE1761

Alternate gene names: 18310743

Gene position: 2040412-2039600 (Counterclockwise)

Preceding gene: 18310744

Following gene: 18310742

Centisome position: 67.31

GC content: 30.14

Gene sequence:

>813_bases
ATGCGGAAGGGAATTACTTCGAAGGGGTTGATTTCAATTATGATGATAAATAAAGTAGAAATATGTGGAGTCAATACAGC
TAAATTACCAGTTTTAAAAGAAAAAGAAATGAAAGAATTATTGCTACAAATGCAAAATGGAGATACAAGTGCTAGAGAGA
AATTTATTAAGGGGAATCTGAGATTAGTGCTTAGTGTAATCCAAAGGTTTAATAATAGAGGCGAAAATGTAGATGATTTA
TTTCAAGTTGGTTGTATAGGACTTATGAAATCTATAGATAACTTTGATTTATCACAAAATGTTAAATTTTCAACTTATGC
TGTGCCAATGATAATAGGAGAAATAAGAAGATATTTAAGGGATAATAACACTATAAGAGTAAGTAGATCTTTAAGAGATA
TAGCTTATAAGGCTTTACTTGTAAGAGATAAGCTAGTAAATGAAAATAATAAGGAACCTACTGTATCTCAAATAGCAAAA
GAGCTTAAAATTCCAAGGGAAGATGTTGTTTTTGCCCTAGATGCAATTCAAGATCCAGTTTCTTTATTTGAGCCTATTTA
TCATGATGGTGGAGATGCAATATATGTAATGGATCAGATAAGTGATTCAAAAAATCAAGATGATAGTTGGCTTGAAAATA
TAGCAATAAAGGAAGCTATGAAGAAATTAAACGATAGAGAAAAACTTATATTGAATTTGAGATTCTTTAATGGAAGAACT
CAAATGGAGGTAGCTGATGAAATTGGTATATCTCAAGCACAAGTTTCAAGGTTGGAAAAAATAGCATTAAAGCATATGAG
GAAGTATGTATAG

Upstream 100 bases:

>100_bases
ATATTTCAAGGTTAGAGAAGAAAATTATAAAAAGATTAAAGAAAGAAATTAACAAAATGATTTAAGAGTATAAAATTAAC
CTCTACAGGTAATAATTAAA

Downstream 100 bases:

>100_bases
AGCTCTTTTGAGCTCTATTTTTTATGGATAAATTAAAAATCTTATGTTATTTAGTATTTTTTACCTCTTCTAATTAATAA
ATATAAAAATTAAACATATA

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRKGITSKGLISIMMINKVEICGVNTAKLPVLKEKEMKELLLQMQNGDTSAREKFIKGNLRLVLSVIQRFNNRGENVDDL
FQVGCIGLMKSIDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNTIRVSRSLRDIAYKALLVRDKLVNENNKEPTVSQIAK
ELKIPREDVVFALDAIQDPVSLFEPIYHDGGDAIYVMDQISDSKNQDDSWLENIAIKEAMKKLNDREKLILNLRFFNGRT
QMEVADEIGISQAQVSRLEKIALKHMRKYV

Sequences:

>Translated_270_residues
MRKGITSKGLISIMMINKVEICGVNTAKLPVLKEKEMKELLLQMQNGDTSAREKFIKGNLRLVLSVIQRFNNRGENVDDL
FQVGCIGLMKSIDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNTIRVSRSLRDIAYKALLVRDKLVNENNKEPTVSQIAK
ELKIPREDVVFALDAIQDPVSLFEPIYHDGGDAIYVMDQISDSKNQDDSWLENIAIKEAMKKLNDREKLILNLRFFNGRT
QMEVADEIGISQAQVSRLEKIALKHMRKYV
>Mature_270_residues
MRKGITSKGLISIMMINKVEICGVNTAKLPVLKEKEMKELLLQMQNGDTSAREKFIKGNLRLVLSVIQRFNNRGENVDDL
FQVGCIGLMKSIDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNTIRVSRSLRDIAYKALLVRDKLVNENNKEPTVSQIAK
ELKIPREDVVFALDAIQDPVSLFEPIYHDGGDAIYVMDQISDSKNQDDSWLENIAIKEAMKKLNDREKLILNLRFFNGRT
QMEVADEIGISQAQVSRLEKIALKHMRKYV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=246, Percent_Identity=28.4552845528455, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789098, Length=263, Percent_Identity=26.9961977186312, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1789871, Length=259, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788231, Length=195, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30937; Mature: 30937

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGITSKGLISIMMINKVEICGVNTAKLPVLKEKEMKELLLQMQNGDTSAREKFIKGNL
CCCCCCCCCCEEEEEECCEEEECCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCHH
RLVLSVIQRFNNRGENVDDLFQVGCIGLMKSIDNFDLSQNVKFSTYAVPMIIGEIRRYLR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHC
DNNTIRVSRSLRDIAYKALLVRDKLVNENNKEPTVSQIAKELKIPREDVVFALDAIQDPV
CCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHH
SLFEPIYHDGGDAIYVMDQISDSKNQDDSWLENIAIKEAMKKLNDREKLILNLRFFNGRT
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
QMEVADEIGISQAQVSRLEKIALKHMRKYV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKGITSKGLISIMMINKVEICGVNTAKLPVLKEKEMKELLLQMQNGDTSAREKFIKGNL
CCCCCCCCCCEEEEEECCEEEECCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCHH
RLVLSVIQRFNNRGENVDDLFQVGCIGLMKSIDNFDLSQNVKFSTYAVPMIIGEIRRYLR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHC
DNNTIRVSRSLRDIAYKALLVRDKLVNENNKEPTVSQIAKELKIPREDVVFALDAIQDPV
CCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHH
SLFEPIYHDGGDAIYVMDQISDSKNQDDSWLENIAIKEAMKKLNDREKLILNLRFFNGRT
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
QMEVADEIGISQAQVSRLEKIALKHMRKYV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961408; 7883192; 11466286 [H]