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Identifier: 18310615

GI number: 18310615

Start: 1905910

End: 1906377

Strand: Reverse

Name: def

Synonym: CPE1633

Alternate gene names: 18310615

Gene position: 1906377-1905910 (Counterclockwise)

Preceding gene: 18310616

Following gene: 18310614

Centisome position: 62.89

GC content: 30.56

Gene sequence:

>468_bases
ATGGCTGTTAAGAAAATAGTTCAAATAGGACACGAAGCTTTAAAAAAGGTTTCAGAACCTGTTAAAGATGTTAATGAAGT
TAAAGGATTAATACAAGATTTAAAAGATACATTAGCAACTGTAGAAGGAATAGGATTAGCTGCACCTCAAATAGCAGTTA
ATAAAAGAGTTGTTTATATAAATTTTGGAGATGGTGAGAATGAATATGTTCTTATAAATCCAGAGGTAACAGGAGTTTCA
AAGGAAACTTATGAAGATTATGAAGGTTGTTTAAGCTATGTAATGCATGAGGGATTAGTTGAAAGACCAAGAGCAGTAAG
AATACAAGCTTTAAATGAAAAGGGAGAATTAAAAGTTTATGAAGCTCAAGATCTTTTAGCAAGATGTTTCTTACATGAGA
TAGATCACTTAGAAGGTATTATGTACGTTGATAGAGCTAAAGAAATGTACGAATTAGTTGAAAAATAA

Upstream 100 bases:

>100_bases
CAATAATATTTTACAGAGAATATAATATATAATTTATTAGTGAAAAATTTATATTTTTATGTTTTAATATATTTATAGGA
ATTTTGAAAGGAGAAGTTAA

Downstream 100 bases:

>100_bases
ATAGCTAGTTTAAGAGTATAGATTTAAAAATCTATACTCTTTTTTTATATTTTTACAAAAAAAGTCTTGATTATCGTTTA
CAAAAGTATTATTATATAGT

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MAVKKIVQIGHEALKKVSEPVKDVNEVKGLIQDLKDTLATVEGIGLAAPQIAVNKRVVYINFGDGENEYVLINPEVTGVS
KETYEDYEGCLSYVMHEGLVERPRAVRIQALNEKGELKVYEAQDLLARCFLHEIDHLEGIMYVDRAKEMYELVEK

Sequences:

>Translated_155_residues
MAVKKIVQIGHEALKKVSEPVKDVNEVKGLIQDLKDTLATVEGIGLAAPQIAVNKRVVYINFGDGENEYVLINPEVTGVS
KETYEDYEGCLSYVMHEGLVERPRAVRIQALNEKGELKVYEAQDLLARCFLHEIDHLEGIMYVDRAKEMYELVEK
>Mature_154_residues
AVKKIVQIGHEALKKVSEPVKDVNEVKGLIQDLKDTLATVEGIGLAAPQIAVNKRVVYINFGDGENEYVLINPEVTGVSK
ETYEDYEGCLSYVMHEGLVERPRAVRIQALNEKGELKVYEAQDLLARCFLHEIDHLEGIMYVDRAKEMYELVEK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=146, Percent_Identity=41.0958904109589, Blast_Score=103, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24645728, Length=163, Percent_Identity=29.4478527607362, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_CLOPE (Q8XJX0)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562549.1
- ProteinModelPortal:   Q8XJX0
- SMR:   Q8XJX0
- GeneID:   989943
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1633
- NMPDR:   fig|195102.1.peg.1696
- HOGENOM:   HBG665227
- OMA:   YQDGDEG
- ProtClustDB:   CLSK519201
- BioCyc:   CPER195102:CPE1633-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 17505; Mature: 17374

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKKIVQIGHEALKKVSEPVKDVNEVKGLIQDLKDTLATVEGIGLAAPQIAVNKRVVYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEE
NFGDGENEYVLINPEVTGVSKETYEDYEGCLSYVMHEGLVERPRAVRIQALNEKGELKVY
EECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCEEEE
EAQDLLARCFLHEIDHLEGIMYVDRAKEMYELVEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVKKIVQIGHEALKKVSEPVKDVNEVKGLIQDLKDTLATVEGIGLAAPQIAVNKRVVYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEE
NFGDGENEYVLINPEVTGVSKETYEDYEGCLSYVMHEGLVERPRAVRIQALNEKGELKVY
EECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCEEEE
EAQDLLARCFLHEIDHLEGIMYVDRAKEMYELVEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842