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The map label for this gene is comEC [H]

Identifier: 18310579

GI number: 18310579

Start: 1870960

End: 1871823

Strand: Direct

Name: comEC [H]

Synonym: CPE1597

Alternate gene names: 18310579

Gene position: 1870960-1871823 (Clockwise)

Preceding gene: 18310573

Following gene: 18310591

Centisome position: 61.72

GC content: 29.05

Gene sequence:

>864_bases
ATGGATAAAAAAAAGAAAATTATTAGCTCAATTATTGGAATTATAGTTGTTCTTTTAGCTGGATATTTTGGAATAGATTT
AACACAAGACTCTAAAGTTCCTAAAGACTCTGAACTTATGATTTCTTATATGGATGTTGGCCAAGGTGATGCAGCATATA
TAAAAGTAAATGGAAATGATATTTTAATAGATGCAGGTCCAAGAAGTAATTCTAAAGAACTATTAGAACAATTAAAAGCT
AAAAACATTGATGATTTTGAATTAGTTATTGCAACTCATCCCCATGAAGATCACATTGGTGGAATGGTTGATGTATTTAA
AGAATATGAAGTAAAGGCTTTCTATTCTCCTAAAATAACTCATACAACTAAAACATATGAAAATCTTGTTAAAGCAGTGA
AAGATGAAGGATTAAAAACTAAAGAATTAAAAGGTGGAATGGTTATAGATTTAGGAGAGGGAGCTAAATTTGAAGTATTT
ACTCCTCAAAAATCAGAGTACGAAGAACTTAACGATTATTCTCCTATAATGAAATTAAGCTTTGGTGACACTTCTTATTT
ATTTACTGGTGATGCTGAAAAGTTAGCTGAGGAAGAAGCCTTAGCTAAGTATAAAACTTCTCTTAATTCAGATGTAATAA
AATTTGGTCATCATGGATCTAGTAGCTCATCAAGCAATGCCTTTATAGAAGCTGTTTCTCCAAAGTATGGAATAATAAGT
TGTGCTAAAGATAATAAATATGGACATCCACATAGAGAAACTTTAGACATAATAAAAAAATATAATATTAAGACCTTTAG
AACCGATACAGATGGAGAAATAATTCTAACTTCAGACGGAAAATCAATAAATTTTAATAAATAA

Upstream 100 bases:

>100_bases
TCTTAATATAAACAATGTACATCTATTAAATCTCATATTAAACTATAATTCTTATTGATGAATTTAATTCTCACAAATAA
AATACAAGGAGATTTATTGC

Downstream 100 bases:

>100_bases
TATTTTCTTAAACTACAAAAGGCACTAGAGTAACTAGTGCCTTTTATTTCTCTGTATTAATACAAATTAAAAACTTTGTA
AATTAGTTATTTTCGTGAGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MDKKKKIISSIIGIIVVLLAGYFGIDLTQDSKVPKDSELMISYMDVGQGDAAYIKVNGNDILIDAGPRSNSKELLEQLKA
KNIDDFELVIATHPHEDHIGGMVDVFKEYEVKAFYSPKITHTTKTYENLVKAVKDEGLKTKELKGGMVIDLGEGAKFEVF
TPQKSEYEELNDYSPIMKLSFGDTSYLFTGDAEKLAEEEALAKYKTSLNSDVIKFGHHGSSSSSSNAFIEAVSPKYGIIS
CAKDNKYGHPHRETLDIIKKYNIKTFRTDTDGEIILTSDGKSINFNK

Sequences:

>Translated_287_residues
MDKKKKIISSIIGIIVVLLAGYFGIDLTQDSKVPKDSELMISYMDVGQGDAAYIKVNGNDILIDAGPRSNSKELLEQLKA
KNIDDFELVIATHPHEDHIGGMVDVFKEYEVKAFYSPKITHTTKTYENLVKAVKDEGLKTKELKGGMVIDLGEGAKFEVF
TPQKSEYEELNDYSPIMKLSFGDTSYLFTGDAEKLAEEEALAKYKTSLNSDVIKFGHHGSSSSSSNAFIEAVSPKYGIIS
CAKDNKYGHPHRETLDIIKKYNIKTFRTDTDGEIILTSDGKSINFNK
>Mature_287_residues
MDKKKKIISSIIGIIVVLLAGYFGIDLTQDSKVPKDSELMISYMDVGQGDAAYIKVNGNDILIDAGPRSNSKELLEQLKA
KNIDDFELVIATHPHEDHIGGMVDVFKEYEVKAFYSPKITHTTKTYENLVKAVKDEGLKTKELKGGMVIDLGEGAKFEVF
TPQKSEYEELNDYSPIMKLSFGDTSYLFTGDAEKLAEEEALAKYKTSLNSDVIKFGHHGSSSSSSNAFIEAVSPKYGIIS
CAKDNKYGHPHRETLDIIKKYNIKTFRTDTDGEIILTSDGKSINFNK

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=233, Percent_Identity=28.3261802575107, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 31884; Mature: 31884

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKKKIISSIIGIIVVLLAGYFGIDLTQDSKVPKDSELMISYMDVGQGDAAYIKVNGND
CCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCHHEEEEEEECCCCCEEEEEECCCE
ILIDAGPRSNSKELLEQLKAKNIDDFELVIATHPHEDHIGGMVDVFKEYEVKAFYSPKIT
EEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHCCHHHHHHHHCCEEEEECCCEE
HTTKTYENLVKAVKDEGLKTKELKGGMVIDLGEGAKFEVFTPQKSEYEELNDYSPIMKLS
ECHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCEEEEECCCCHHHHHHHCCCCEEEEE
FGDTSYLFTGDAEKLAEEEALAKYKTSLNSDVIKFGHHGSSSSSSNAFIEAVSPKYGIIS
CCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCCCCEEE
CAKDNKYGHPHRETLDIIKKYNIKTFRTDTDGEIILTSDGKSINFNK
EECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MDKKKKIISSIIGIIVVLLAGYFGIDLTQDSKVPKDSELMISYMDVGQGDAAYIKVNGND
CCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCHHEEEEEEECCCCCEEEEEECCCE
ILIDAGPRSNSKELLEQLKAKNIDDFELVIATHPHEDHIGGMVDVFKEYEVKAFYSPKIT
EEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHCCHHHHHHHHCCEEEEECCCEE
HTTKTYENLVKAVKDEGLKTKELKGGMVIDLGEGAKFEVFTPQKSEYEELNDYSPIMKLS
ECHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCEEEEECCCCHHHHHHHCCCCEEEEE
FGDTSYLFTGDAEKLAEEEALAKYKTSLNSDVIKFGHHGSSSSSSNAFIEAVSPKYGIIS
CCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCCCCEEE
CAKDNKYGHPHRETLDIIKKYNIKTFRTDTDGEIILTSDGKSINFNK
EECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]