BacMap

Definition	Clostridium perfringens str. 13, complete genome.
Accession	NC_003366
Length	3,031,430

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The map label for this gene is ppiB [H]

Identifier: 18310549

GI number: 18310549

Start: 1836335

End: 1836967

Strand: Reverse

Name: ppiB [H]

Synonym: CPE1567

Alternate gene names: 18310549

Gene position: 1836967-1836335 (Counterclockwise)

Preceding gene: 18310550

Following gene: 18310548

Centisome position: 60.6

GC content: 34.76

Gene sequence:

>633_bases
ATGAGTTTATTAAAGAAAGTTAGCAAAATAGTAGTAGGAGTTACAATGGCATCTTTACTTTTAATAGGATGTGGAGGGAC
AGGTAAAAAGGCAAAGGAAAATATACCAGAGGATAAATTACCTGTAGCTACTATTGAAGTTAAGAATTTTGGAACTATAG
AAGCAGAGTTATATCCAAACAAAGCTCCAAATACCGTTGATAACTTTATAAGCCTAGCTAATAGTGGATTTTATGATGGC
TTAACTATCCATAGAATAGTTAAGGGATTTGTGCTTCAGGGTGGAGATCCAAAGGGAACAGGTACAGGTGGACCAGGATA
TATGATAAAGGGTGAATTTTCTTCTAATGGAGTAGAAAATGATATTAAGCATGAAGAGGGAATATTATCAATGGCAAGGT
CTCAAAATCCTGATAGTGCAGGAAGTCAATTCTTTATTGTTACAAAAGAAGCACCACATTTAAATGGTCAATATGCAGCC
TTTGGAAAAGTTACTAAGGGATTAGATGTGGTTCATGAAATAGAAAAGGTTTCAGTAGGGGCAAATGATAAACCAGTTGA
GGATGTTGTTATAGAATCAATTAAGGTGGATACAAAGGGAATAGAGTATAAGGAGCCTGAAAAGGTTAAATAA

Upstream 100 bases:

>100_bases
TATAGAAACAAATTAATAGCTTATATGACATAAATTTAAATGGTTTAGATGATATAATTTAAAACATATTAGTAATAAAT
TCTAAGAAAGGGGAATAAAA

Downstream 100 bases:

>100_bases
AGGAATTTTAATTTATTAATTACCTGTATTTTAAGATAAGTTTTAAATAGATTTATTGTTTAAAACTTATCTTTTATAAT
ATTGGAATTTAAAAAGTCTT

Product: peptidyl-prolyl cis-trans isomerase, cyclophilin-type

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSLLKKVSKIVVGVTMASLLLIGCGGTGKKAKENIPEDKLPVATIEVKNFGTIEAELYPNKAPNTVDNFISLANSGFYDG
LTIHRIVKGFVLQGGDPKGTGTGGPGYMIKGEFSSNGVENDIKHEEGILSMARSQNPDSAGSQFFIVTKEAPHLNGQYAA
FGKVTKGLDVVHEIEKVSVGANDKPVEDVVIESIKVDTKGIEYKEPEKVK

Sequences:

>Translated_210_residues
MSLLKKVSKIVVGVTMASLLLIGCGGTGKKAKENIPEDKLPVATIEVKNFGTIEAELYPNKAPNTVDNFISLANSGFYDG
LTIHRIVKGFVLQGGDPKGTGTGGPGYMIKGEFSSNGVENDIKHEEGILSMARSQNPDSAGSQFFIVTKEAPHLNGQYAA
FGKVTKGLDVVHEIEKVSVGANDKPVEDVVIESIKVDTKGIEYKEPEKVK
>Mature_209_residues
SLLKKVSKIVVGVTMASLLLIGCGGTGKKAKENIPEDKLPVATIEVKNFGTIEAELYPNKAPNTVDNFISLANSGFYDGL
TIHRIVKGFVLQGGDPKGTGTGGPGYMIKGEFSSNGVENDIKHEEGILSMARSQNPDSAGSQFFIVTKEAPHLNGQYAAF
GKVTKGLDVVHEIEKVSVGANDKPVEDVVIESIKVDTKGIEYKEPEKVK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI24308049, Length=150, Percent_Identity=42.6666666666667, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI7706339, Length=159, Percent_Identity=39.622641509434, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI19557636, Length=148, Percent_Identity=39.8648648648649, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4758950, Length=156, Percent_Identity=41.025641025641, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI64276486, Length=152, Percent_Identity=37.5, Blast_Score=91, Evalue=5e-19,
Organism=Homo sapiens, GI4505991, Length=158, Percent_Identity=40.5063291139241, Blast_Score=91, Evalue=6e-19,
Organism=Homo sapiens, GI5454154, Length=154, Percent_Identity=36.3636363636364, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI6631100, Length=166, Percent_Identity=36.7469879518072, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI22547212, Length=153, Percent_Identity=41.1764705882353, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI7657473, Length=153, Percent_Identity=41.1764705882353, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI22547215, Length=153, Percent_Identity=41.1764705882353, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI10863927, Length=133, Percent_Identity=42.8571428571429, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI310127646, Length=146, Percent_Identity=38.3561643835616, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI310114371, Length=146, Percent_Identity=38.3561643835616, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI310119221, Length=146, Percent_Identity=38.3561643835616, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI42560244, Length=155, Percent_Identity=37.4193548387097, Blast_Score=81, Evalue=5e-16,
Organism=Homo sapiens, GI20911035, Length=149, Percent_Identity=32.8859060402685, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI178057341, Length=156, Percent_Identity=37.8205128205128, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4826932, Length=155, Percent_Identity=38.0645161290323, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI5174637, Length=170, Percent_Identity=37.6470588235294, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI256017253, Length=156, Percent_Identity=37.8205128205128, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI221139811, Length=156, Percent_Identity=37.8205128205128, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI256017251, Length=156, Percent_Identity=37.8205128205128, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI45439316, Length=154, Percent_Identity=38.3116883116883, Blast_Score=77, Evalue=9e-15,
Organism=Homo sapiens, GI209417936, Length=154, Percent_Identity=37.012987012987, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI30102944, Length=154, Percent_Identity=37.012987012987, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI219842199, Length=154, Percent_Identity=37.012987012987, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI303305009, Length=153, Percent_Identity=37.9084967320261, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI5031987, Length=146, Percent_Identity=36.986301369863, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI310110104, Length=142, Percent_Identity=37.3239436619718, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI150418007, Length=132, Percent_Identity=40.9090909090909, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI310129267, Length=130, Percent_Identity=40, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI310120620, Length=130, Percent_Identity=40, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI169163387, Length=136, Percent_Identity=35.2941176470588, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI169164185, Length=136, Percent_Identity=35.2941176470588, Blast_Score=71, Evalue=5e-13,
Organism=Homo sapiens, GI169163623, Length=136, Percent_Identity=35.2941176470588, Blast_Score=71, Evalue=5e-13,
Organism=Homo sapiens, GI310132444, Length=142, Percent_Identity=36.6197183098592, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI310113152, Length=142, Percent_Identity=36.6197183098592, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1786736, Length=162, Percent_Identity=37.6543209876543, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789763, Length=156, Percent_Identity=41.6666666666667, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17539496, Length=155, Percent_Identity=39.3548387096774, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17506311, Length=150, Percent_Identity=38.6666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17532641, Length=165, Percent_Identity=37.5757575757576, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17551284, Length=153, Percent_Identity=41.1764705882353, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17551282, Length=153, Percent_Identity=41.1764705882353, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71980594, Length=147, Percent_Identity=38.0952380952381, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71980590, Length=137, Percent_Identity=40.1459854014599, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17552780, Length=147, Percent_Identity=43.5374149659864, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506307, Length=160, Percent_Identity=41.25, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17552784, Length=157, Percent_Identity=37.5796178343949, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71997782, Length=118, Percent_Identity=42.3728813559322, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17532643, Length=176, Percent_Identity=32.9545454545455, Blast_Score=80, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17559074, Length=160, Percent_Identity=38.75, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17565860, Length=142, Percent_Identity=37.3239436619718, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17566266, Length=143, Percent_Identity=37.7622377622378, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17539498, Length=137, Percent_Identity=36.4963503649635, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17552782, Length=151, Percent_Identity=37.0860927152318, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32563659, Length=150, Percent_Identity=30.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323562, Length=147, Percent_Identity=41.4965986394558, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320510, Length=212, Percent_Identity=34.4339622641509, Blast_Score=89, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321848, Length=150, Percent_Identity=44, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320359, Length=133, Percent_Identity=40.6015037593985, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323246, Length=175, Percent_Identity=35.4285714285714, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322492, Length=190, Percent_Identity=32.6315789473684, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19922376, Length=152, Percent_Identity=39.4736842105263, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17986117, Length=150, Percent_Identity=42.6666666666667, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20130249, Length=147, Percent_Identity=45.578231292517, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24652460, Length=148, Percent_Identity=35.8108108108108, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24650807, Length=153, Percent_Identity=35.9477124183007, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357249, Length=119, Percent_Identity=39.4957983193277, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136360, Length=181, Percent_Identity=34.2541436464088, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24586038, Length=166, Percent_Identity=34.9397590361446, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922912, Length=153, Percent_Identity=39.8692810457516, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45549139, Length=131, Percent_Identity=39.6946564885496, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24664125, Length=151, Percent_Identity=36.4238410596026, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21357783, Length=151, Percent_Identity=36.4238410596026, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17647301, Length=149, Percent_Identity=34.2281879194631, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 780 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 22420; Mature: 22289

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLKKVSKIVVGVTMASLLLIGCGGTGKKAKENIPEDKLPVATIEVKNFGTIEAELYPN
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEECCCCEEEEEECCC
KAPNTVDNFISLANSGFYDGLTIHRIVKGFVLQGGDPKGTGTGGPGYMIKGEFSSNGVEN
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCC
DIKHEEGILSMARSQNPDSAGSQFFIVTKEAPHLNGQYAAFGKVTKGLDVVHEIEKVSVG
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
ANDKPVEDVVIESIKVDTKGIEYKEPEKVK
CCCCCHHHHHHHHHEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SLLKKVSKIVVGVTMASLLLIGCGGTGKKAKENIPEDKLPVATIEVKNFGTIEAELYPN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEECCCCEEEEEECCC
KAPNTVDNFISLANSGFYDGLTIHRIVKGFVLQGGDPKGTGTGGPGYMIKGEFSSNGVEN
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCC
DIKHEEGILSMARSQNPDSAGSQFFIVTKEAPHLNGQYAAFGKVTKGLDVVHEIEKVSVG
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
ANDKPVEDVVIESIKVDTKGIEYKEPEKVK
CCCCCHHHHHHHHHEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9317025; 9665876 [H]