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The map label for this gene is yeiE [H]

Identifier: 18310457

GI number: 18310457

Start: 1731671

End: 1732555

Strand: Reverse

Name: yeiE [H]

Synonym: CPE1475

Alternate gene names: 18310457

Gene position: 1732555-1731671 (Counterclockwise)

Preceding gene: 18310458

Following gene: 18310455

Centisome position: 57.15

GC content: 26.33

Gene sequence:

>885_bases
ATGAATTTTAGAAAGTTAAAAATATTTTTTGAAACAGCTAAATGTTTAAATATGACTAAAGTAGCTAAGTCTATGTATAT
AAGTCAGCCATCTATAAGTCAAGCCATAGCTGAATTAGAAAGTGATCTTGATGTTAAACTTTTTGATAGAATAGGTAAAA
GACTTTATCTTACTCATGAGGGTGAAGTATACTTTGAATATAGTAGAAGAATTTTAAACCTTTATGAAGAGGCAAATAGT
ACCATTAGAAGTAGTAAAGAAGGTCAGAAAGGGAAAATAGTTATAGGTGCAAGTACAACAATAGGTATATATATACTTCC
AGAGTTAATAAAGGAATTCAATGAGTTACATAAAAATATAGAAATCTCTTTAATAATAGAGAATACACAGTTAATAGAAG
AGCTTATTATGGAAAATAAGGTTGATATTGCTTTAGTAGAAGGATATGTAAAATCAGATGAATTAGAAGTTTTTGATATA
GGAAAGGATGAGTTAATATTTATAGCAAATCCTAATAATCCTATATTTTCAAAGGATAAAATAACTTTAAAAGACTTAGA
AGATGAAAAATTTATTATGAGGGAGCCTGGAAGTGGTACAAGAGAAATTATAGAAAATTATCTTATAAACAAAGGGTGCA
ACTACAATGTATATATGGAACTTGGAAATACAGAGGCTATTGTAAGAGTTGTAGAAACAGGATTAGGCATAGCCTGTGTT
TCTTGTAAAGCTATAGATGAAAGAATTAATGAAGGCTTAATCAAAGAAATAAAAATAGATGATATTAAGGTAAGTAGAGA
TTTATATCTTATATATCATAAGGATAAATTTATTTCTAAAAACTTAGAAATATTTATAGATAAAATTAAAAGTTCAGATA
TATAA

Upstream 100 bases:

>100_bases
CAAAAGAAGAAAAGAAATCAGAGGCTGATATGCAAGATGATGGCCCTGTTATTGATGTTGATTTTGAAGAAGTAAAAGAT
GATAAATAAGGATTGATTTA

Downstream 100 bases:

>100_bases
AAAAAGGATAGTAATACTTATTTTTTAGTATTACTATCCTTTCTTTTCTTTTATAGTAGATAAAAGAAAATTGATATAAA
GTGACATAGGCTTCCTAATA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNFRKLKIFFETAKCLNMTKVAKSMYISQPSISQAIAELESDLDVKLFDRIGKRLYLTHEGEVYFEYSRRILNLYEEANS
TIRSSKEGQKGKIVIGASTTIGIYILPELIKEFNELHKNIEISLIIENTQLIEELIMENKVDIALVEGYVKSDELEVFDI
GKDELIFIANPNNPIFSKDKITLKDLEDEKFIMREPGSGTREIIENYLINKGCNYNVYMELGNTEAIVRVVETGLGIACV
SCKAIDERINEGLIKEIKIDDIKVSRDLYLIYHKDKFISKNLEIFIDKIKSSDI

Sequences:

>Translated_294_residues
MNFRKLKIFFETAKCLNMTKVAKSMYISQPSISQAIAELESDLDVKLFDRIGKRLYLTHEGEVYFEYSRRILNLYEEANS
TIRSSKEGQKGKIVIGASTTIGIYILPELIKEFNELHKNIEISLIIENTQLIEELIMENKVDIALVEGYVKSDELEVFDI
GKDELIFIANPNNPIFSKDKITLKDLEDEKFIMREPGSGTREIIENYLINKGCNYNVYMELGNTEAIVRVVETGLGIACV
SCKAIDERINEGLIKEIKIDDIKVSRDLYLIYHKDKFISKNLEIFIDKIKSSDI
>Mature_294_residues
MNFRKLKIFFETAKCLNMTKVAKSMYISQPSISQAIAELESDLDVKLFDRIGKRLYLTHEGEVYFEYSRRILNLYEEANS
TIRSSKEGQKGKIVIGASTTIGIYILPELIKEFNELHKNIEISLIIENTQLIEELIMENKVDIALVEGYVKSDELEVFDI
GKDELIFIANPNNPIFSKDKITLKDLEDEKFIMREPGSGTREIIENYLINKGCNYNVYMELGNTEAIVRVVETGLGIACV
SCKAIDERINEGLIKEIKIDDIKVSRDLYLIYHKDKFISKNLEIFIDKIKSSDI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=34.0136054421769, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=25.4355400696864, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788748, Length=295, Percent_Identity=25.4237288135593, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI145693105, Length=280, Percent_Identity=25, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=29.5546558704453, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=27.681660899654, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787879, Length=290, Percent_Identity=22.4137931034483, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790399, Length=295, Percent_Identity=23.0508474576271, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789204, Length=254, Percent_Identity=21.6535433070866, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=25.8196721311475, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790208, Length=240, Percent_Identity=25.8333333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787530, Length=291, Percent_Identity=21.6494845360825, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082132, Length=277, Percent_Identity=22.0216606498195, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787806, Length=295, Percent_Identity=20.3389830508475, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI87081904, Length=220, Percent_Identity=23.6363636363636, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788297, Length=188, Percent_Identity=25, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=275, Percent_Identity=23.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=206, Percent_Identity=24.2718446601942, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367136, Length=277, Percent_Identity=22.0216606498195, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=21.9178082191781, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790262, Length=251, Percent_Identity=18.7250996015936, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=20.2090592334495, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33772; Mature: 33772

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRKLKIFFETAKCLNMTKVAKSMYISQPSISQAIAELESDLDVKLFDRIGKRLYLTHE
CCCEEEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCEEEEEEC
GEVYFEYSRRILNLYEEANSTIRSSKEGQKGKIVIGASTTIGIYILPELIKEFNELHKNI
CCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHCCE
EISLIIENTQLIEELIMENKVDIALVEGYVKSDELEVFDIGKDELIFIANPNNPIFSKDK
EEEEEECCHHHHHHHHHCCCCCEEEEECEECCCCEEEEECCCCCEEEEECCCCCCCCCCC
ITLKDLEDEKFIMREPGSGTREIIENYLINKGCNYNVYMELGNTEAIVRVVETGLGIACV
EEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCEEEE
SCKAIDERINEGLIKEIKIDDIKVSRDLYLIYHKDKFISKNLEIFIDKIKSSDI
HHHHHHHHHCCCCHHHEEECCEEEECEEEEEEECCHHHHCCHHHEEEECCCCCC
>Mature Secondary Structure
MNFRKLKIFFETAKCLNMTKVAKSMYISQPSISQAIAELESDLDVKLFDRIGKRLYLTHE
CCCEEEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCEEEEEEC
GEVYFEYSRRILNLYEEANSTIRSSKEGQKGKIVIGASTTIGIYILPELIKEFNELHKNI
CCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHCCE
EISLIIENTQLIEELIMENKVDIALVEGYVKSDELEVFDIGKDELIFIANPNNPIFSKDK
EEEEEECCHHHHHHHHHCCCCCEEEEECEECCCCEEEEECCCCCEEEEECCCCCCCCCCC
ITLKDLEDEKFIMREPGSGTREIIENYLINKGCNYNVYMELGNTEAIVRVVETGLGIACV
EEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCEEEE
SCKAIDERINEGLIKEIKIDDIKVSRDLYLIYHKDKFISKNLEIFIDKIKSSDI
HHHHHHHHHCCCCHHHEEECCEEEECEEEEEEECCHHHHCCHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]