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The map label for this gene is manX [C]

Identifier: 18310447

GI number: 18310447

Start: 1721989

End: 1722486

Strand: Reverse

Name: manX [C]

Synonym: CPE1465

Alternate gene names: 18310447

Gene position: 1722486-1721989 (Counterclockwise)

Preceding gene: 18310448

Following gene: 18310446

Centisome position: 56.82

GC content: 31.12

Gene sequence:

>498_bases
ATGATAACTCAAATACGTGTAGATGACCGTTTAATACATGGACAAGTAGCAGTAGTATGGACAAAGGAGCTAAATGCACC
ACTTTTAGTAGTAGCAAATGATGAAGCAGCAAAAAATGAAGTTATGCAAATGACTTTAAAAATGGCTGTACCAAATGGAA
TGAAACTTTTAATACGTTCAGTTGAAGATGCTATAAAAGTTTTTAATGATCCTCGTGGTAAGGATAAAAGAATGTTTGTT
ATAGTAAACAGTGTATCAGATGCTACAAAGATTGCTAAAGAAGTTAGTGATATAGAATCTGTAAACGTTGCTAATGCAGG
ACGTTTTGATAAATCTAATCCAAAAGACAAAGTAATGGTATTTTCAAGTGTTCAATTAAATCCAGATGAATTAAAAGCTG
CTAAGGAATTATCAGAGCTAACTAATGTTAAATCTTATAATCAAGTATTACCATCAAATCCACAGCTTGATTTAAAGAAA
GCTTTAGCACAATTTTAA

Upstream 100 bases:

>100_bases
TTATATTTATATGAAATGATAAATTTTTTTAAAGTGATAGTAATCGTTTAAAAAGAAAAAATTAACAAAATAGTATTTTG
AATTTTAGGAGGAAAAAAAT

Downstream 100 bases:

>100_bases
TATAATATTTGATTAGATAAACTAGACTTAGTTTAAATTAAAATTTTAGAATAGTTTATAAATCGATTTACATTGAATTT
TAAAAAAAATAAAGCTTAAT

Product: PTS system protein

Products: NA

Alternate protein names: PTS system EIIB component [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEVMQMTLKMAVPNGMKLLIRSVEDAIKVFNDPRGKDKRMFV
IVNSVSDATKIAKEVSDIESVNVANAGRFDKSNPKDKVMVFSSVQLNPDELKAAKELSELTNVKSYNQVLPSNPQLDLKK
ALAQF

Sequences:

>Translated_165_residues
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEVMQMTLKMAVPNGMKLLIRSVEDAIKVFNDPRGKDKRMFV
IVNSVSDATKIAKEVSDIESVNVANAGRFDKSNPKDKVMVFSSVQLNPDELKAAKELSELTNVKSYNQVLPSNPQLDLKK
ALAQF
>Mature_165_residues
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEVMQMTLKMAVPNGMKLLIRSVEDAIKVFNDPRGKDKRMFV
IVNSVSDATKIAKEVSDIESVNVANAGRFDKSNPKDKVMVFSSVQLNPDELKAAKELSELTNVKSYNQVLPSNPQLDLKK
ALAQF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=133, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=9e-15,
Organism=Escherichia coli, GI87082217, Length=158, Percent_Identity=28.4810126582279, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18320; Mature: 18320

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEVMQMTLKMAVPNGMKLLIRS
CCEEEECCCEEECCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHH
VEDAIKVFNDPRGKDKRMFVIVNSVSDATKIAKEVSDIESVNVANAGRFDKSNPKDKVMV
HHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
FSSVQLNPDELKAAKELSELTNVKSYNQVLPSNPQLDLKKALAQF
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEVMQMTLKMAVPNGMKLLIRS
CCEEEECCCEEECCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHH
VEDAIKVFNDPRGKDKRMFVIVNSVSDATKIAKEVSDIESVNVANAGRFDKSNPKDKVMV
HHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
FSSVQLNPDELKAAKELSELTNVKSYNQVLPSNPQLDLKKALAQF
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA