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The map label for this gene is yitJ [H]

Identifier: 18310399

GI number: 18310399

Start: 1671289

End: 1672128

Strand: Reverse

Name: yitJ [H]

Synonym: CPE1417

Alternate gene names: 18310399

Gene position: 1672128-1671289 (Counterclockwise)

Preceding gene: 18310401

Following gene: 18310398

Centisome position: 55.16

GC content: 26.9

Gene sequence:

>840_bases
ATGAAAAATTTAAATTTGAAAAATGGAGTAATAATAGCAGATGGAGCTATGGGAACTAGAATAATGGAATTAGGAGTAAA
TTTAAAAGAGACTCCATCAGAACTTTTAAATATTAAAAAGCCTGAATTAATAGAAAAAATTCATAGAGAGTATATAGAAT
CTGGAGCTAACTTAATACTTTCAAACACATTTATGTGTAATATTATAAATGCAAAAAGGAATAATTATAATTTAGAGGAA
ATTATAGAGGCAGGAATTAGTATTGCTAAAAAAGCATGTGGATATCATGGCTTAGTTGCTTTAGATATAGGCCCCCTTTC
ATATTATATAGAAGAAAATGATTCTTCTTTTAAAGAAATTGTTTATGAAAATACTGAAAGAATAATTAATGCTTCAAAAG
ATAAATTTGATTTAGTTATTTTTGAAACCTTAGGAAGTTTAAAAGAAGGTGAATTTGCAGTTAAAAAAGCAAAAACTTTA
ACAGATAAAAAGGTTATATGTTCTTTTACTTTAGCTTATAAAAAGGATATTCCTAATTTCATAAAAAATATGGTTTCAAC
TTTAGAGCCTTTAGGAGTAGATGCCTTAGGAATAAATTGTACAGGGTACGAAGAAATACTAATGGCCTTAGATATACTTA
AGGAAAATACAAATCTTCCTATAATGATAAAGGCTAATTTAGGAATACCAAAAAAAGTAGGGGAAGAGCTTGTATATGAT
AAGACTTTAGAAGAATTTAAAAATTTATCTAAAAGAGCCCTTGAAAAGGGAGTTAATATAATAGGAGGATGTTGTGGAAC
AACTCCAGAATATATAAGAGCTATTTGTAATTTGAAATAG

Upstream 100 bases:

>100_bases
ATCAAAATACAGTTATTTTATTTGAGATAATTTAAATAAAAAATAAAGCAGAATAACTTATTTTAGAATATAAATTACTT
TGATTGGAGAATACATTAAG

Downstream 100 bases:

>100_bases
AGTATAAAAGAATAATGGTATAATAAGGGGATATAATTATGGGTGAAAATAAGAGGGAAAAAGTTTTTTTAGAGTTTCCT
ATTATTGAGACTGAAAGATT

Product: hypothetical protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKNLNLKNGVIIADGAMGTRIMELGVNLKETPSELLNIKKPELIEKIHREYIESGANLILSNTFMCNIINAKRNNYNLEE
IIEAGISIAKKACGYHGLVALDIGPLSYYIEENDSSFKEIVYENTERIINASKDKFDLVIFETLGSLKEGEFAVKKAKTL
TDKKVICSFTLAYKKDIPNFIKNMVSTLEPLGVDALGINCTGYEEILMALDILKENTNLPIMIKANLGIPKKVGEELVYD
KTLEEFKNLSKRALEKGVNIIGGCCGTTPEYIRAICNLK

Sequences:

>Translated_279_residues
MKNLNLKNGVIIADGAMGTRIMELGVNLKETPSELLNIKKPELIEKIHREYIESGANLILSNTFMCNIINAKRNNYNLEE
IIEAGISIAKKACGYHGLVALDIGPLSYYIEENDSSFKEIVYENTERIINASKDKFDLVIFETLGSLKEGEFAVKKAKTL
TDKKVICSFTLAYKKDIPNFIKNMVSTLEPLGVDALGINCTGYEEILMALDILKENTNLPIMIKANLGIPKKVGEELVYD
KTLEEFKNLSKRALEKGVNIIGGCCGTTPEYIRAICNLK
>Mature_279_residues
MKNLNLKNGVIIADGAMGTRIMELGVNLKETPSELLNIKKPELIEKIHREYIESGANLILSNTFMCNIINAKRNNYNLEE
IIEAGISIAKKACGYHGLVALDIGPLSYYIEENDSSFKEIVYENTERIINASKDKFDLVIFETLGSLKEGEFAVKKAKTL
TDKKVICSFTLAYKKDIPNFIKNMVSTLEPLGVDALGINCTGYEEILMALDILKENTNLPIMIKANLGIPKKVGEELVYD
KTLEEFKNLSKRALEKGVNIIGGCCGTTPEYIRAICNLK

Specific function: Catalyzes Methyl Transfer From S-Methylmethionine Or S- Adenosylmethionine (Less Efficient) To Homocysteine, Selenohomocysteine And Less Efficiently Selenocysteine. [C]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786456, Length=306, Percent_Identity=25.4901960784314, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535405, Length=312, Percent_Identity=28.525641025641, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322966, Length=309, Percent_Identity=21.6828478964401, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 31048; Mature: 31048

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLNLKNGVIIADGAMGTRIMELGVNLKETPSELLNIKKPELIEKIHREYIESGANLIL
CCCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
SNTFMCNIINAKRNNYNLEEIIEAGISIAKKACGYHGLVALDIGPLSYYIEENDSSFKEI
ECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCHHHHH
VYENTERIINASKDKFDLVIFETLGSLKEGEFAVKKAKTLTDKKVICSFTLAYKKDIPNF
HHHHHHHHHCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IKNMVSTLEPLGVDALGINCTGYEEILMALDILKENTNLPIMIKANLGIPKKVGEELVYD
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
KTLEEFKNLSKRALEKGVNIIGGCCGTTPEYIRAICNLK
HHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKNLNLKNGVIIADGAMGTRIMELGVNLKETPSELLNIKKPELIEKIHREYIESGANLIL
CCCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
SNTFMCNIINAKRNNYNLEEIIEAGISIAKKACGYHGLVALDIGPLSYYIEENDSSFKEI
ECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCHHHHH
VYENTERIINASKDKFDLVIFETLGSLKEGEFAVKKAKTLTDKKVICSFTLAYKKDIPNF
HHHHHHHHHCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IKNMVSTLEPLGVDALGINCTGYEEILMALDILKENTNLPIMIKANLGIPKKVGEELVYD
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
KTLEEFKNLSKRALEKGVNIIGGCCGTTPEYIRAICNLK
HHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]