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The map label for this gene is ddl

Identifier: 18310297

GI number: 18310297

Start: 1555884

End: 1556789

Strand: Reverse

Name: ddl

Synonym: CPE1315

Alternate gene names: 18310297

Gene position: 1556789-1555884 (Counterclockwise)

Preceding gene: 18310298

Following gene: 18310296

Centisome position: 51.35

GC content: 30.24

Gene sequence:

>906_bases
ATGAAAGTTGGAGTAATAATGGGAGGCATTTCTTCAGAAAGAGAGATATCAATACAAAGTGGTAATTCTGTAGTTCATGC
CTTAGATAAAGATAAATATGAAGCTATTCCTATAGTTTTAAATGAAAAAGAAGATTTAATAGAAAAGGTTAAAGGAATTG
ATTTTGCTCTTTTAGCTTTACATGGAAAATTTGGTGAAGATGGAACTGTTCAATCTGTTCTTAAAACTTTAGGAATACCA
TTTTCAGGATGTGGTCCATTAAGTAGTGCTATATGTATGGATAAGGACATGACAAAGAGAATTTTAGCCTTTGGAAATGT
TAGAACAGCAAGATGGGAAATGGTTTCATCAGTAGATGAAATAGACTATGAAAAAATAGAAAATTTAGGTTATCCAGTAT
TTGTAAAACCTAATAATGGGGGATCATCAGTTGCCACAACTTTAGTTGAATCTAAAGAAGCAGTTAAGGATGCTGTGTTA
GAAGCCTTAAAATATGATACAGAAGTAATGATTGAAGAATACATTAAAGGTGATGAAATAACTTGTCCTATAATTGATGG
AAAAATGTTACCTGTTTTAGCTATAAAACCAAAGGGGAAATTCTTTGATATAGCTTCAAAGTATGGAGATGGAGGAGCAG
ATGAATTTATAGTTGAATTAAATGAAGATCTTCATAAAGAAGTAGAAAAAATGGCCTTAGAAACTTACAAGTTATTAAAG
TGTGATGTATATGCTAGGGTAGACATGCTAGTAAAGGATAATATTCCATATGTATTAGAAGTAAATACATTACCTGGTAT
GACAAAGAATAGTTTATTCCCTAAAAGTGCAGCAGGAATAAATATGAGTTTTGAAGAACTTTTAGATACAATAATAGAAA
AATCTTTAAAGGTTAATAGAGAATAA

Upstream 100 bases:

>100_bases
TGATTTCATAAACTTTCTATTTTAAAAAGATAATGATATAATATTAAGGGCTTGTTTAAAAAAGACAAGTCCTTAATTAT
TTCTATTTGGAGGTAAAAAT

Downstream 100 bases:

>100_bases
TTTTATTTAGTAACAACTTTCCCTGTAATATTATATTTTATATAGTAAGAACTACTTTGCAGGGAGAGTATTAATGCAAG
ATAAAAATCCATTAAGTACA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKVGVIMGGISSEREISIQSGNSVVHALDKDKYEAIPIVLNEKEDLIEKVKGIDFALLALHGKFGEDGTVQSVLKTLGIP
FSGCGPLSSAICMDKDMTKRILAFGNVRTARWEMVSSVDEIDYEKIENLGYPVFVKPNNGGSSVATTLVESKEAVKDAVL
EALKYDTEVMIEEYIKGDEITCPIIDGKMLPVLAIKPKGKFFDIASKYGDGGADEFIVELNEDLHKEVEKMALETYKLLK
CDVYARVDMLVKDNIPYVLEVNTLPGMTKNSLFPKSAAGINMSFEELLDTIIEKSLKVNRE

Sequences:

>Translated_301_residues
MKVGVIMGGISSEREISIQSGNSVVHALDKDKYEAIPIVLNEKEDLIEKVKGIDFALLALHGKFGEDGTVQSVLKTLGIP
FSGCGPLSSAICMDKDMTKRILAFGNVRTARWEMVSSVDEIDYEKIENLGYPVFVKPNNGGSSVATTLVESKEAVKDAVL
EALKYDTEVMIEEYIKGDEITCPIIDGKMLPVLAIKPKGKFFDIASKYGDGGADEFIVELNEDLHKEVEKMALETYKLLK
CDVYARVDMLVKDNIPYVLEVNTLPGMTKNSLFPKSAAGINMSFEELLDTIIEKSLKVNRE
>Mature_301_residues
MKVGVIMGGISSEREISIQSGNSVVHALDKDKYEAIPIVLNEKEDLIEKVKGIDFALLALHGKFGEDGTVQSVLKTLGIP
FSGCGPLSSAICMDKDMTKRILAFGNVRTARWEMVSSVDEIDYEKIENLGYPVFVKPNNGGSSVATTLVESKEAVKDAVL
EALKYDTEVMIEEYIKGDEITCPIIDGKMLPVLAIKPKGKFFDIASKYGDGGADEFIVELNEDLHKEVEKMALETYKLLK
CDVYARVDMLVKDNIPYVLEVNTLPGMTKNSLFPKSAAGINMSFEELLDTIIEKSLKVNRE

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=302, Percent_Identity=35.4304635761589, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1786579, Length=350, Percent_Identity=30, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLA_CLOPE (Q8XKS9)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562231.1
- ProteinModelPortal:   Q8XKS9
- SMR:   Q8XKS9
- GeneID:   989624
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1315
- NMPDR:   fig|195102.1.peg.1378
- HOGENOM:   HBG427092
- OMA:   DEFIVEL
- PhylomeDB:   Q8XKS9
- ProtClustDB:   PRK01372
- BioCyc:   CPER195102:CPE1315-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33183; Mature: 33183

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGVIMGGISSEREISIQSGNSVVHALDKDKYEAIPIVLNEKEDLIEKVKGIDFALLAL
CEEEEEECCCCCCCEEEEECCCEEEEECCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEE
HGKFGEDGTVQSVLKTLGIPFSGCGPLSSAICMDKDMTKRILAFGNVRTARWEMVSSVDE
ECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
IDYEKIENLGYPVFVKPNNGGSSVATTLVESKEAVKDAVLEALKYDTEVMIEEYIKGDEI
CCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCE
TCPIIDGKMLPVLAIKPKGKFFDIASKYGDGGADEFIVELNEDLHKEVEKMALETYKLLK
EEEEECCCCEEEEEECCCCCEEEHHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHH
CDVYARVDMLVKDNIPYVLEVNTLPGMTKNSLFPKSAAGINMSFEELLDTIIEKSLKVNR
HHHHHHEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
E
C
>Mature Secondary Structure
MKVGVIMGGISSEREISIQSGNSVVHALDKDKYEAIPIVLNEKEDLIEKVKGIDFALLAL
CEEEEEECCCCCCCEEEEECCCEEEEECCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEE
HGKFGEDGTVQSVLKTLGIPFSGCGPLSSAICMDKDMTKRILAFGNVRTARWEMVSSVDE
ECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
IDYEKIENLGYPVFVKPNNGGSSVATTLVESKEAVKDAVLEALKYDTEVMIEEYIKGDEI
CCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCE
TCPIIDGKMLPVLAIKPKGKFFDIASKYGDGGADEFIVELNEDLHKEVEKMALETYKLLK
EEEEECCCCEEEEEECCCCCEEEHHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHH
CDVYARVDMLVKDNIPYVLEVNTLPGMTKNSLFPKSAAGINMSFEELLDTIIEKSLKVNR
HHHHHHEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842