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The map label for this gene is (tet [H]

Identifier: 18310264

GI number: 18310264

Start: 1510515

End: 1511903

Strand: Reverse

Name: (tet [H]

Synonym: CPE1282

Alternate gene names: 18310264

Gene position: 1511903-1510515 (Counterclockwise)

Preceding gene: 18310266

Following gene: 18310263

Centisome position: 49.87

GC content: 27.86

Gene sequence:

>1389_bases
ATGGACATTTCACTTAATACTTTAGAAAATGTTGAAGAAGTAAAAATAAAAAAAGCGGTACCAGCTGTATTAGCTTTAAT
GATTTTTGCTTTAGTTATTGACAACTCATTTAAAATTATTTCACCAGATTTGGCTAAAGCTTTTAACATATCAGCTACTG
CTGTTAGTTGGCAAGTAACTTTAGCTGGATTAGTTATTGGTATTGGAGCAGTTGTATACGCTTCTTTATCTGATTCAATA
AGTGTTAGAACCTTATTGGTACTAGGAATTATATTAATATGTGTTGGTTCACTATTAGGATTTATTTTTCAGAAATCATT
TTTAATTATTGTTATTTCAAGAATAATTCAGTCAGCTGGATTAGGAAGTGCAGAAACATTATATGTAATATTTATAGCAA
AGTATTTTAAAGAGAGTGAACATAAAAAATACTTAGGATTTAGTACAAGTAGTTATGCTATTTCACAAGTAATTGGTACA
ATTACTGGAGGATACATATCAACATACTTAAGTTGGCAAGTACTATTTTTAATTCCATTATTAACTTTAGTAATATTACC
TTTTATATTAAAATATATTCCTAAGGAAAAGAATAAAGCTGGTAATGTGGATTATATAGGATTTATATTAGTTGCAACAG
TTGCAGCAACATTAATGATCTTTGTTTCAGGATTCCAATGGGGAGTTTTAATAGGATTTATAGTTGCGCTTGCATTATTC
CTTATATACATAAGTAAAAATGAAAATGCTTTTATAAGCATTAAGTTTTTCACAAATAGAAACTTTATATCAGTATTACT
TGTAGCCTTTGTTATATACAGTGTACAGCTTGCATTTATATTTATGTTCCCATTCTTAGTTGAATCTATATACAATTATA
GATTAGACCAAATTTCATTACTTTTAGTTCCTTCATATGTTTTAGCTGCTACTGTAGGAGCTTTATCAGGAAAGGTTTCT
AAGAAAATAGGAAGTAAGCAATGTATTCAAATGGCTATAGTAGGTATAATAGTGAGTCTTATTATAGGAGCTTTATTTGT
AAAAACATCAGTTTTAGTATTTATTTTTGTAATGATACTATTCTCAAGTTCCTTTGCTTTAATGTATGCACCTATGATTG
ATACATTAACAAGAACAATACCTAAAGAAAAAACAGGAACTGCTATTGGTTTCTATAATTTCTGTTTAAATATAGCTACT
TCAATAGGAATAACATATGTAGCAATATTTATGGAAACTAAGTATTTAGGAAAGAACTTTACTGGACTTTTCAGTGACTT
AGTAGCTATACAATATAGTAACGTTTTAATAGTATTAGCAGTTATATCTTTAGTAGCACTTGTATTATACTGGACTTTAA
TCGGAAGAAGAGAGAAAAAGGACATATAA

Upstream 100 bases:

>100_bases
TTGAGGGAGGATGAAGTAAAAAGAGTAGACAAGTTTATAATGATTATTAGTAAGCTATTATTTAAGAAGCACAAATGAAA
AAGAAAAGAGAGGATTTAAA

Downstream 100 bases:

>100_bases
ATAAAACTTTAGAATCTTATAAATAATAGTTTAAAAAACTAAGTGTTAAAGAGCCTATAGGGTGGTCAATATAAAAGACT
ATCTTATAGGTTTTTTGTTT

Product: transmembrane protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Tetracycline efflux protein [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MDISLNTLENVEEVKIKKAVPAVLALMIFALVIDNSFKIISPDLAKAFNISATAVSWQVTLAGLVIGIGAVVYASLSDSI
SVRTLLVLGIILICVGSLLGFIFQKSFLIIVISRIIQSAGLGSAETLYVIFIAKYFKESEHKKYLGFSTSSYAISQVIGT
ITGGYISTYLSWQVLFLIPLLTLVILPFILKYIPKEKNKAGNVDYIGFILVATVAATLMIFVSGFQWGVLIGFIVALALF
LIYISKNENAFISIKFFTNRNFISVLLVAFVIYSVQLAFIFMFPFLVESIYNYRLDQISLLLVPSYVLAATVGALSGKVS
KKIGSKQCIQMAIVGIIVSLIIGALFVKTSVLVFIFVMILFSSSFALMYAPMIDTLTRTIPKEKTGTAIGFYNFCLNIAT
SIGITYVAIFMETKYLGKNFTGLFSDLVAIQYSNVLIVLAVISLVALVLYWTLIGRREKKDI

Sequences:

>Translated_462_residues
MDISLNTLENVEEVKIKKAVPAVLALMIFALVIDNSFKIISPDLAKAFNISATAVSWQVTLAGLVIGIGAVVYASLSDSI
SVRTLLVLGIILICVGSLLGFIFQKSFLIIVISRIIQSAGLGSAETLYVIFIAKYFKESEHKKYLGFSTSSYAISQVIGT
ITGGYISTYLSWQVLFLIPLLTLVILPFILKYIPKEKNKAGNVDYIGFILVATVAATLMIFVSGFQWGVLIGFIVALALF
LIYISKNENAFISIKFFTNRNFISVLLVAFVIYSVQLAFIFMFPFLVESIYNYRLDQISLLLVPSYVLAATVGALSGKVS
KKIGSKQCIQMAIVGIIVSLIIGALFVKTSVLVFIFVMILFSSSFALMYAPMIDTLTRTIPKEKTGTAIGFYNFCLNIAT
SIGITYVAIFMETKYLGKNFTGLFSDLVAIQYSNVLIVLAVISLVALVLYWTLIGRREKKDI
>Mature_462_residues
MDISLNTLENVEEVKIKKAVPAVLALMIFALVIDNSFKIISPDLAKAFNISATAVSWQVTLAGLVIGIGAVVYASLSDSI
SVRTLLVLGIILICVGSLLGFIFQKSFLIIVISRIIQSAGLGSAETLYVIFIAKYFKESEHKKYLGFSTSSYAISQVIGT
ITGGYISTYLSWQVLFLIPLLTLVILPFILKYIPKEKNKAGNVDYIGFILVATVAATLMIFVSGFQWGVLIGFIVALALF
LIYISKNENAFISIKFFTNRNFISVLLVAFVIYSVQLAFIFMFPFLVESIYNYRLDQISLLLVPSYVLAATVGALSGKVS
KKIGSKQCIQMAIVGIIVSLIIGALFVKTSVLVFIFVMILFSSSFALMYAPMIDTLTRTIPKEKTGTAIGFYNFCLNIAT
SIGITYVAIFMETKYLGKNFTGLFSDLVAIQYSNVLIVLAVISLVALVLYWTLIGRREKKDI

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=375, Percent_Identity=21.8666666666667, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50744; Mature: 50744

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50850 MFS ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISLNTLENVEEVKIKKAVPAVLALMIFALVIDNSFKIISPDLAKAFNISATAVSWQVT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCHHHHHHHHH
LAGLVIGIGAVVYASLSDSISVRTLLVLGIILICVGSLLGFIFQKSFLIIVISRIIQSAG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LGSAETLYVIFIAKYFKESEHKKYLGFSTSSYAISQVIGTITGGYISTYLSWQVLFLIPL
CCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLVILPFILKYIPKEKNKAGNVDYIGFILVATVAATLMIFVSGFQWGVLIGFIVALALF
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LIYISKNENAFISIKFFTNRNFISVLLVAFVIYSVQLAFIFMFPFLVESIYNYRLDQISL
HHHEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LLVPSYVLAATVGALSGKVSKKIGSKQCIQMAIVGIIVSLIIGALFVKTSVLVFIFVMIL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSSSFALMYAPMIDTLTRTIPKEKTGTAIGFYNFCLNIATSIGITYVAIFMETKYLGKNF
HCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TGLFSDLVAIQYSNVLIVLAVISLVALVLYWTLIGRREKKDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDISLNTLENVEEVKIKKAVPAVLALMIFALVIDNSFKIISPDLAKAFNISATAVSWQVT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCHHHHHHHHH
LAGLVIGIGAVVYASLSDSISVRTLLVLGIILICVGSLLGFIFQKSFLIIVISRIIQSAG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LGSAETLYVIFIAKYFKESEHKKYLGFSTSSYAISQVIGTITGGYISTYLSWQVLFLIPL
CCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLVILPFILKYIPKEKNKAGNVDYIGFILVATVAATLMIFVSGFQWGVLIGFIVALALF
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LIYISKNENAFISIKFFTNRNFISVLLVAFVIYSVQLAFIFMFPFLVESIYNYRLDQISL
HHHEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LLVPSYVLAATVGALSGKVSKKIGSKQCIQMAIVGIIVSLIIGALFVKTSVLVFIFVMIL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSSSFALMYAPMIDTLTRTIPKEKTGTAIGFYNFCLNIATSIGITYVAIFMETKYLGKNF
HCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TGLFSDLVAIQYSNVLIVLAVISLVALVLYWTLIGRREKKDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6657777; 3142848; 8234490 [H]