Click here to switch to the map view.

The map label for this gene is cbiH [H]

Identifier: 18310202

GI number: 18310202

Start: 1423826

End: 1424548

Strand: Reverse

Name: cbiH [H]

Synonym: CPE1220

Alternate gene names: 18310202

Gene position: 1424548-1423826 (Counterclockwise)

Preceding gene: 18310203

Following gene: 18310201

Centisome position: 46.99

GC content: 31.12

Gene sequence:

>723_bases
ATGGGAAAATTATATGTAATTGGAATTGGACCAGGTGGACTAGATGAAATGACTTTAAGAGCAGTTAAAGCTATTGAAGA
ATCTGATATAATAGTTGGTTACACTAAATATATAGAAATGGTTAAAGACTTAATAAAAGATAAGGAAATCTTTAAAACTG
GTATGCGTGGAGAAGAGGAAAGATGCAGAGAAGCCTTAGAACTTAGTAAGGATAAAAAAGTTGCTTTAATAAGTACTGGA
GATTCAGGAATTTATGGCATGGCTGGACTTATCTTAGAGATGAGAAAAGATGAAAATGTTGAAATAATACCTGGTATTAC
AGCATCTAGTGCAGCAGGATCAGTTTTAGGTGCTCCTTTAATGCATGATAACTGTAACATAAGTTTAAGTGATTTAATGA
CTCCTTATGAAGATATAAAGAAAAGAGTTCGTTTAGCAGCAGAGGGAGATTTCGTAATTTCTCTTTACAATCCAAAGAGT
AAGGGAAGACCACACTATTTAAGAGAGTGTGTTGATATAATTAAGGAATTTAGAGGAGAGGAAACTCCTATAGCAGTTGT
TAGAAATGCTTTAAGAGAAGGAGAAAGTAAAGAAATATTTACTTTAAAAGACTTTAATGATGAAGTAGTTGATATGTTCT
CAATAGTAATCATAGGAAACTCAAAAAGTTATATTAAAGATGGATATTTTGTAACTCCAAGAGGATATAAAATAAAAAAA
TAA

Upstream 100 bases:

>100_bases
CTTAGAAATTGTTTAAAAGTATGGTAGAAAAGTAAAAGCTTTAATAAGCTTATAACCATAATACTTTACAACTTAAGCTA
CATAATAAGGAGGAATAATA

Downstream 100 bases:

>100_bases
AACTAATGGGGTGAGAATGTGATTGCACTTATATTAGGAACTTCAGAAGGAAGAGAGTTTTTACAAAAGCTTAAGGGATT
TGAAGAAAAAATAGTTATAT

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MGKLYVIGIGPGGLDEMTLRAVKAIEESDIIVGYTKYIEMVKDLIKDKEIFKTGMRGEEERCREALELSKDKKVALISTG
DSGIYGMAGLILEMRKDENVEIIPGITASSAAGSVLGAPLMHDNCNISLSDLMTPYEDIKKRVRLAAEGDFVISLYNPKS
KGRPHYLRECVDIIKEFRGEETPIAVVRNALREGESKEIFTLKDFNDEVVDMFSIVIIGNSKSYIKDGYFVTPRGYKIKK

Sequences:

>Translated_240_residues
MGKLYVIGIGPGGLDEMTLRAVKAIEESDIIVGYTKYIEMVKDLIKDKEIFKTGMRGEEERCREALELSKDKKVALISTG
DSGIYGMAGLILEMRKDENVEIIPGITASSAAGSVLGAPLMHDNCNISLSDLMTPYEDIKKRVRLAAEGDFVISLYNPKS
KGRPHYLRECVDIIKEFRGEETPIAVVRNALREGESKEIFTLKDFNDEVVDMFSIVIIGNSKSYIKDGYFVTPRGYKIKK
>Mature_239_residues
GKLYVIGIGPGGLDEMTLRAVKAIEESDIIVGYTKYIEMVKDLIKDKEIFKTGMRGEEERCREALELSKDKKVALISTGD
SGIYGMAGLILEMRKDENVEIIPGITASSAAGSVLGAPLMHDNCNISLSDLMTPYEDIKKRVRLAAEGDFVISLYNPKSK
GRPHYLRECVDIIKEFRGEETPIAVVRNALREGESKEIFTLKDFNDEVVDMFSIVIIGNSKSYIKDGYFVTPRGYKIKK

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 26770; Mature: 26639

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLYVIGIGPGGLDEMTLRAVKAIEESDIIVGYTKYIEMVKDLIKDKEIFKTGMRGEEE
CCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHH
RCREALELSKDKKVALISTGDSGIYGMAGLILEMRKDENVEIIPGITASSAAGSVLGAPL
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHCCCE
MHDNCNISLSDLMTPYEDIKKRVRLAAEGDFVISLYNPKSKGRPHYLRECVDIIKEFRGE
ECCCCCEEHHHHCCCHHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
ETPIAVVRNALREGESKEIFTLKDFNDEVVDMFSIVIIGNSKSYIKDGYFVTPRGYKIKK
CCHHHHHHHHHHCCCCCEEEEEECCCHHHHEEEEEEEECCCCCHHCCCEEECCCCCEECC
>Mature Secondary Structure 
GKLYVIGIGPGGLDEMTLRAVKAIEESDIIVGYTKYIEMVKDLIKDKEIFKTGMRGEEE
CEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHH
RCREALELSKDKKVALISTGDSGIYGMAGLILEMRKDENVEIIPGITASSAAGSVLGAPL
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHCCCE
MHDNCNISLSDLMTPYEDIKKRVRLAAEGDFVISLYNPKSKGRPHYLRECVDIIKEFRGE
ECCCCCEEHHHHCCCHHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
ETPIAVVRNALREGESKEIFTLKDFNDEVVDMFSIVIIGNSKSYIKDGYFVTPRGYKIKK
CCHHHHHHHHHHCCCCCEEEEEECCCHHHHEEEEEEEECCCCCHHCCCEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]