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The map label for this gene is cbiJ [H]

Identifier: 18310201

GI number: 18310201

Start: 1423043

End: 1423807

Strand: Reverse

Name: cbiJ [H]

Synonym: CPE1219

Alternate gene names: 18310201

Gene position: 1423807-1423043 (Counterclockwise)

Preceding gene: 18310202

Following gene: 18310200

Centisome position: 46.97

GC content: 28.76

Gene sequence:

>765_bases
GTGATTGCACTTATATTAGGAACTTCAGAAGGAAGAGAGTTTTTACAAAAGCTTAAGGGATTTGAAGAAAAAATAGTTAT
ATCTACAGCTACAGAATATGGTGGGGAACTTTTAAAAGAATTCCCTGTTGCATTTATGAATACAAAACCTTTAGATGAAG
AAGGACTTTTAAATTTTGCTAAGGAATTTAATTTAAAAGGAATAGTTGATGCTTCTCATCCTTATGCAAAGAATGTTAGT
GAAAACACAATAAAAGTATGTAAGAAATTAAATATAGAATACATAAGATATGAAAGAAATGGTGTACTAAAAGAGTTAGA
CAATCCTAATATTATTAGAATAAGTGGATTAGAGAAACTTAAAGAATTAGACTCTACTATTAATGGACCAATTTTAAACA
CCACAGGAAGTAATGGAGTAAAGCAGATATTAGAATCTTCTCTTGAAAATAGAGTTATTCATAGAGTATTACCTTCAAAA
AAGGTTATGAATGATCTTATAGATTTAGGGGTTAAGATAGAAGATATAATTGCTATAAAAGGCCCTATTGGCTATGAACT
TAACAAGGCTTTTATATATGAATACAAGGCAAAGGCTCTTTTAACTAAAGATAGTGGTGAAAGAGGAGGAGCTTTAGAAA
AAGCAAAGGCTGCACTAGATTCAAATATTAAATTAATAATAGTTGAAAAGCCTAAGGTGGATTATGGTAGATGTTTTTCT
GATATAGATGAAACAGTAAAATACGTTAAAAATATTTTGAAATAA

Upstream 100 bases:

>100_bases
AATAGTAATCATAGGAAACTCAAAAAGTTATATTAAAGATGGATATTTTGTAACTCCAAGAGGATATAAAATAAAAAAAT
AAAACTAATGGGGTGAGAAT

Downstream 100 bases:

>100_bases
CAGGGGGATAAAATGAGAAGAAAAAGAATTTTAGGTTTAGCTACTATAGGATTAGTATTTTCCTTAGCTTTAGCAGGATG
TACTGGAAGTTCAAAGGGAA

Product: cobalt-precorrin-6x reductase

Products: Precorrin 6A; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MIALILGTSEGREFLQKLKGFEEKIVISTATEYGGELLKEFPVAFMNTKPLDEEGLLNFAKEFNLKGIVDASHPYAKNVS
ENTIKVCKKLNIEYIRYERNGVLKELDNPNIIRISGLEKLKELDSTINGPILNTTGSNGVKQILESSLENRVIHRVLPSK
KVMNDLIDLGVKIEDIIAIKGPIGYELNKAFIYEYKAKALLTKDSGERGGALEKAKAALDSNIKLIIVEKPKVDYGRCFS
DIDETVKYVKNILK

Sequences:

>Translated_254_residues
MIALILGTSEGREFLQKLKGFEEKIVISTATEYGGELLKEFPVAFMNTKPLDEEGLLNFAKEFNLKGIVDASHPYAKNVS
ENTIKVCKKLNIEYIRYERNGVLKELDNPNIIRISGLEKLKELDSTINGPILNTTGSNGVKQILESSLENRVIHRVLPSK
KVMNDLIDLGVKIEDIIAIKGPIGYELNKAFIYEYKAKALLTKDSGERGGALEKAKAALDSNIKLIIVEKPKVDYGRCFS
DIDETVKYVKNILK
>Mature_254_residues
MIALILGTSEGREFLQKLKGFEEKIVISTATEYGGELLKEFPVAFMNTKPLDEEGLLNFAKEFNLKGIVDASHPYAKNVS
ENTIKVCKKLNIEYIRYERNGVLKELDNPNIIRISGLEKLKELDSTINGPILNTTGSNGVKQILESSLENRVIHRVLPSK
KVMNDLIDLGVKIEDIIAIKGPIGYELNKAFIYEYKAKALLTKDSGERGGALEKAKAALDSNIKLIIVEKPKVDYGRCFS
DIDETVKYVKNILK

Specific function: Catalyzes the reduction of the macrocycle of cobalt- precorrin-6X into cobalt-precorrin-6Y [H]

COG id: COG2099

COG function: function code H; Precorrin-6x reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin-6x reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003723 [H]

Pfam domain/function: PF02571 CbiJ [H]

EC number: 1.3.1.54

Molecular weight: Translated: 28389; Mature: 28389

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALILGTSEGREFLQKLKGFEEKIVISTATEYGGELLKEFPVAFMNTKPLDEEGLLNFA
CEEEEEECCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCHHHCCCCCCCHHHHHHHH
KEFNLKGIVDASHPYAKNVSENTIKVCKKLNIEYIRYERNGVLKELDNPNIIRISGLEKL
HHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHCCCCCEEEECCHHHH
KELDSTINGPILNTTGSNGVKQILESSLENRVIHRVLPSKKVMNDLIDLGVKIEDIIAIK
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEHEEEEC
GPIGYELNKAFIYEYKAKALLTKDSGERGGALEKAKAALDSNIKLIIVEKPKVDYGRCFS
CCCCCCCCHHHEEEEHHHEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
DIDETVKYVKNILK
HHHHHHHHHHHHHC
>Mature Secondary Structure
MIALILGTSEGREFLQKLKGFEEKIVISTATEYGGELLKEFPVAFMNTKPLDEEGLLNFA
CEEEEEECCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCHHHCCCCCCCHHHHHHHH
KEFNLKGIVDASHPYAKNVSENTIKVCKKLNIEYIRYERNGVLKELDNPNIIRISGLEKL
HHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHCCCCCEEEECCHHHH
KELDSTINGPILNTTGSNGVKQILESSLENRVIHRVLPSKKVMNDLIDLGVKIEDIIAIK
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEHEEEEC
GPIGYELNKAFIYEYKAKALLTKDSGERGGALEKAKAALDSNIKLIIVEKPKVDYGRCFS
CCCCCCCCHHHEEEEHHHEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
DIDETVKYVKNILK
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 6B; NADP+

Specific reaction: precorrin-6B + NADP+ = precorrin-6A + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]