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The map label for this gene is rluB [H]

Identifier: 18310062

GI number: 18310062

Start: 1284275

End: 1284985

Strand: Direct

Name: rluB [H]

Synonym: CPE1080

Alternate gene names: 18310062

Gene position: 1284275-1284985 (Clockwise)

Preceding gene: 18310060

Following gene: 18310063

Centisome position: 42.37

GC content: 30.1

Gene sequence:

>711_bases
ATGGAAGAAAGATTGCAAAAATACATGGCGAGGTGTGGAGTAGCATCAAGAAGAAAATGTGAAGAAATAATTTGCAGTGG
AAAAGTAAAGGTTAATGACACTGTTGTTGACTCCCTTGGGACTAAGGTTGATCCATCAGTAGATGTTGTTAGCGTTAATA
ATAAAGTAATACGTCCTGAGGAGAATAAAGTATATATAATGCTAAATAAGCCTGAAGGATATGTTACAACCAATAAAGAT
GAAAAAAATAGAAAGACCATATTAGATATAGTAAAGGTGAATGAGAGAATATATCCTATAGGACGTCTTGACTATGACAG
TTCAGGATTACTTTTATTAACTAATGATGGAGATGTATACAATAAGATAATACATCCAAGAGTTAATTTAAATAAAGAAT
ATATTGCCTTAGTAAAAGGAATTTTTACAGAGGAAGAAATGAATAAATTTAGAAAAGGCGTAGATATAGGAGACTATAAA
ACAGCAGAATGTGAAATAGAATTACTAGAAAAATACACAAATAGCTGTAAAGTTAAAATAATCATTCATGAAGGCAAAAA
TAGACAGATAAGAAGAATGTGTGCTGCCTTTAATCATGAAGTTGTTTCTTTAAAAAGAGAAGCTATTGGAAGCTTGACCT
TAAAGAATTTAAAGAAAGGAGAATGGAGATATTTAAGTAGTTCAGAAGAGAATTATATAAAATCTCTATAG

Upstream 100 bases:

>100_bases
GTTTTTTTCAGAAAAAATGCAATTATTCGAAATAAATATTGCATTTTACACTATAGTTATTTAAAATTATTAATAGAAAC
TATCATATGGAGTGTGAAAA

Downstream 100 bases:

>100_bases
TAATTATGTTTAAATATGTATCTGATGTAAGTAATATATATCATAATATAATAACTTCTTTCATAGAAAATAAAAATATA
GCAATAGATGCTACCTTAGG

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MEERLQKYMARCGVASRRKCEEIICSGKVKVNDTVVDSLGTKVDPSVDVVSVNNKVIRPEENKVYIMLNKPEGYVTTNKD
EKNRKTILDIVKVNERIYPIGRLDYDSSGLLLLTNDGDVYNKIIHPRVNLNKEYIALVKGIFTEEEMNKFRKGVDIGDYK
TAECEIELLEKYTNSCKVKIIIHEGKNRQIRRMCAAFNHEVVSLKREAIGSLTLKNLKKGEWRYLSSSEENYIKSL

Sequences:

>Translated_236_residues
MEERLQKYMARCGVASRRKCEEIICSGKVKVNDTVVDSLGTKVDPSVDVVSVNNKVIRPEENKVYIMLNKPEGYVTTNKD
EKNRKTILDIVKVNERIYPIGRLDYDSSGLLLLTNDGDVYNKIIHPRVNLNKEYIALVKGIFTEEEMNKFRKGVDIGDYK
TAECEIELLEKYTNSCKVKIIIHEGKNRQIRRMCAAFNHEVVSLKREAIGSLTLKNLKKGEWRYLSSSEENYIKSL
>Mature_236_residues
MEERLQKYMARCGVASRRKCEEIICSGKVKVNDTVVDSLGTKVDPSVDVVSVNNKVIRPEENKVYIMLNKPEGYVTTNKD
EKNRKTILDIVKVNERIYPIGRLDYDSSGLLLLTNDGDVYNKIIHPRVNLNKEYIALVKGIFTEEEMNKFRKGVDIGDYK
TAECEIELLEKYTNSCKVKIIIHEGKNRQIRRMCAAFNHEVVSLKREAIGSLTLKNLKKGEWRYLSSSEENYIKSL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=228, Percent_Identity=39.9122807017544, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=32.4675324675325, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=31.5573770491803, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI87081838, Length=173, Percent_Identity=35.2601156069364, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27141; Mature: 27141

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERLQKYMARCGVASRRKCEEIICSGKVKVNDTVVDSLGTKVDPSVDVVSVNNKVIRPE
CHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECHHHHHHCCCCCCCCEEEEECCCEEECCC
ENKVYIMLNKPEGYVTTNKDEKNRKTILDIVKVNERIYPIGRLDYDSSGLLLLTNDGDVY
CCEEEEEEECCCCCEECCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCHHH
NKIIHPRVNLNKEYIALVKGIFTEEEMNKFRKGVDIGDYKTAECEIELLEKYTNSCKVKI
HHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
IIHEGKNRQIRRMCAAFNHEVVSLKREAIGSLTLKNLKKGEWRYLSSSEENYIKSL
EEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHCC
>Mature Secondary Structure
MEERLQKYMARCGVASRRKCEEIICSGKVKVNDTVVDSLGTKVDPSVDVVSVNNKVIRPE
CHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECHHHHHHCCCCCCCCEEEEECCCEEECCC
ENKVYIMLNKPEGYVTTNKDEKNRKTILDIVKVNERIYPIGRLDYDSSGLLLLTNDGDVY
CCEEEEEEECCCCCEECCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCHHH
NKIIHPRVNLNKEYIALVKGIFTEEEMNKFRKGVDIGDYKTAECEIELLEKYTNSCKVKI
HHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
IIHEGKNRQIRRMCAAFNHEVVSLKREAIGSLTLKNLKKGEWRYLSSSEENYIKSL
EEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]