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The map label for this gene is adh1 [H]

Identifier: 18309874

GI number: 18309874

Start: 1094185

End: 1095315

Strand: Direct

Name: adh1 [H]

Synonym: CPE0892

Alternate gene names: 18309874

Gene position: 1094185-1095315 (Clockwise)

Preceding gene: 18309873

Following gene: 18309875

Centisome position: 36.09

GC content: 29.89

Gene sequence:

>1131_bases
ATGAAAAGGTTTAAGATTAAGACAGAAATACTTTTTGGAGAAGGAGCCTTAGATTACCTAAAGGAACTTAAGAATAAAAA
AGTTTTTATAGTAACAGATCCTTTTATGGTTAAGTCAGGGACTATAGAAAAATTAACTAATAACCTTAAATCATCTGAGG
TTTTTGTATTTAGTGAAATAGTTCCAGATCCACCTATTGAGTTAGTTGTAAGTGGAATAGAAATGCTTAAAGATTTTAAT
CCTGATGTTGTTATTGCTTTAGGGGGAGGGTCTGCTATAGATGCAACTAAGGCAATAATAGATTTTTCTAAAAAATTAGT
TAAATTAAATGATATTAAGTTTATAGCTATACCAACAACAAGTGGTACAGGCTCAGAAGTAACTTCATTTTCCGTAATAA
CAGATAAGCAAAAAGGAGTTAAATATCCTTTAGTATCAGATGAATTATTACCAGACATAGCAATTTTAGATCCAGAACTT
GTTAAAACAGTTCCAAATTTTATAACTGCTGATACAGGAATGGATGTACTTACTCATGCATTAGAAGCATATGTTTCTAA
AAATGCAACAGACTTTTCAGATGCTTTAGCTGAAAAAGCAGCTACTTTAGTTTTTAAATATTTATTAAAAGCTTATGAAA
ATGGAAATGATATAGAAGCTAGGGAAAAAATGCATAATGCTTCTTGTTTAGCCGGGTTAGCCTTTAATGAGGCTTCTTTA
GGAGTAAACCATGGTATTGCCCATGTTTTAGGAGGAAAATTCCATGTTCCTCATGGAAGAACTAACTCAATATTATTACC
ACATGTAATAGAATATAATGCAAACATAACAAGCTATAATTCTAAGGAATTTAGTGAAGCTGCAGAAAAATATGCTAAAA
TAGCTAAAATGGTTGGATTACAAGGATCAAATACTAGAGGATTAGTAAAAAATCTTGTAAATGAAATTAAAAAGATGCAA
AAGGCAATGAATATGCCTACAACTTTAAGAGAATGTAAAGTTAATTGTGATGAACTAAAGAGTTTAGAGAATGAAATAGC
TCAATTAGCATTAAAGGATGCATGTACAGAAAGTAATCCAAGAGTGCCAGATGAAAATGATATAATATCTATTTTAAACA
AAATTAAATAG

Upstream 100 bases:

>100_bases
ATATTAAACAAGAAAGATCAACAAAAAAACTACCCGTAAACTAAAAATATACTTATAAATAAGCTCTTACCCATAATTAA
TAAAAAGAGGGGAGCTAAAA

Downstream 100 bases:

>100_bases
TTTTTTTATAAGCTTCAAAATAGGACTGATATGCTTAGAAATTATCCTTTTTTGGAGCTTATTTTAGATAGGCATAGATA
TATACTTAATAAAGAATCAG

Product: NADPH-dependent butanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKRFKIKTEILFGEGALDYLKELKNKKVFIVTDPFMVKSGTIEKLTNNLKSSEVFVFSEIVPDPPIELVVSGIEMLKDFN
PDVVIALGGGSAIDATKAIIDFSKKLVKLNDIKFIAIPTTSGTGSEVTSFSVITDKQKGVKYPLVSDELLPDIAILDPEL
VKTVPNFITADTGMDVLTHALEAYVSKNATDFSDALAEKAATLVFKYLLKAYENGNDIEAREKMHNASCLAGLAFNEASL
GVNHGIAHVLGGKFHVPHGRTNSILLPHVIEYNANITSYNSKEFSEAAEKYAKIAKMVGLQGSNTRGLVKNLVNEIKKMQ
KAMNMPTTLRECKVNCDELKSLENEIAQLALKDACTESNPRVPDENDIISILNKIK

Sequences:

>Translated_376_residues
MKRFKIKTEILFGEGALDYLKELKNKKVFIVTDPFMVKSGTIEKLTNNLKSSEVFVFSEIVPDPPIELVVSGIEMLKDFN
PDVVIALGGGSAIDATKAIIDFSKKLVKLNDIKFIAIPTTSGTGSEVTSFSVITDKQKGVKYPLVSDELLPDIAILDPEL
VKTVPNFITADTGMDVLTHALEAYVSKNATDFSDALAEKAATLVFKYLLKAYENGNDIEAREKMHNASCLAGLAFNEASL
GVNHGIAHVLGGKFHVPHGRTNSILLPHVIEYNANITSYNSKEFSEAAEKYAKIAKMVGLQGSNTRGLVKNLVNEIKKMQ
KAMNMPTTLRECKVNCDELKSLENEIAQLALKDACTESNPRVPDENDIISILNKIK
>Mature_376_residues
MKRFKIKTEILFGEGALDYLKELKNKKVFIVTDPFMVKSGTIEKLTNNLKSSEVFVFSEIVPDPPIELVVSGIEMLKDFN
PDVVIALGGGSAIDATKAIIDFSKKLVKLNDIKFIAIPTTSGTGSEVTSFSVITDKQKGVKYPLVSDELLPDIAILDPEL
VKTVPNFITADTGMDVLTHALEAYVSKNATDFSDALAEKAATLVFKYLLKAYENGNDIEAREKMHNASCLAGLAFNEASL
GVNHGIAHVLGGKFHVPHGRTNSILLPHVIEYNANITSYNSKEFSEAAEKYAKIAKMVGLQGSNTRGLVKNLVNEIKKMQ
KAMNMPTTLRECKVNCDELKSLENEIAQLALKDACTESNPRVPDENDIISILNKIK

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=318, Percent_Identity=27.6729559748428, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1787493, Length=408, Percent_Identity=37.5, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI87082107, Length=388, Percent_Identity=34.2783505154639, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI48994951, Length=369, Percent_Identity=35.230352303523, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789163, Length=392, Percent_Identity=32.6530612244898, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789386, Length=285, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17537053, Length=290, Percent_Identity=26.551724137931, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321181, Length=389, Percent_Identity=34.9614395886889, Blast_Score=219, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24657991, Length=412, Percent_Identity=24.0291262135922, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 41194; Mature: 41194

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFKIKTEILFGEGALDYLKELKNKKVFIVTDPFMVKSGTIEKLTNNLKSSEVFVFSEI
CCCEEEEEEEEECCCHHHHHHHHCCCEEEEEECCEEECCCCHHHHHCCCCCCCEEEEECC
VPDPPIELVVSGIEMLKDFNPDVVIALGGGSAIDATKAIIDFSKKLVKLNDIKFIAIPTT
CCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEECCEEEEEEECC
SGTGSEVTSFSVITDKQKGVKYPLVSDELLPDIAILDPELVKTVPNFITADTGMDVLTHA
CCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHH
LEAYVSKNATDFSDALAEKAATLVFKYLLKAYENGNDIEAREKMHNASCLAGLAFNEASL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHEECCCCC
GVNHGIAHVLGGKFHVPHGRTNSILLPHVIEYNANITSYNSKEFSEAAEKYAKIAKMVGL
CHHHCHHHHHCCEEECCCCCCCCEECCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
QGSNTRGLVKNLVNEIKKMQKAMNMPTTLRECKVNCDELKSLENEIAQLALKDACTESNP
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RVPDENDIISILNKIK
CCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKRFKIKTEILFGEGALDYLKELKNKKVFIVTDPFMVKSGTIEKLTNNLKSSEVFVFSEI
CCCEEEEEEEEECCCHHHHHHHHCCCEEEEEECCEEECCCCHHHHHCCCCCCCEEEEECC
VPDPPIELVVSGIEMLKDFNPDVVIALGGGSAIDATKAIIDFSKKLVKLNDIKFIAIPTT
CCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEECCEEEEEEECC
SGTGSEVTSFSVITDKQKGVKYPLVSDELLPDIAILDPELVKTVPNFITADTGMDVLTHA
CCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHH
LEAYVSKNATDFSDALAEKAATLVFKYLLKAYENGNDIEAREKMHNASCLAGLAFNEASL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHEECCCCC
GVNHGIAHVLGGKFHVPHGRTNSILLPHVIEYNANITSYNSKEFSEAAEKYAKIAKMVGL
CHHHCHHHHHCCEEECCCCCCCCEECCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
QGSNTRGLVKNLVNEIKKMQKAMNMPTTLRECKVNCDELKSLENEIAQLALKDACTESNP
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RVPDENDIISILNKIK
CCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]