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The map label for this gene is nplT [H]

Identifier: 18309787

GI number: 18309787

Start: 988114

End: 989946

Strand: Direct

Name: nplT [H]

Synonym: CPE0805

Alternate gene names: 18309787

Gene position: 988114-989946 (Clockwise)

Preceding gene: 18309786

Following gene: 18309788

Centisome position: 32.6

GC content: 30.28

Gene sequence:

>1833_bases
ATGGGTAAAATTACAAGAGAGGCAATACATCATATAGCTCAAAGCAATTATTCTTATGGATATGATAATGAAACTTTGCA
TTTAAGAGTTAAGACTAAAAAAGGTGAAGTTAATAAAGTGGAAATAAGAATTGGAGATCCTTACATATGGGATGAAGGTG
GTTGTGATGGAGGAAATATGAATGCCACTGGAGGACGATGGACAGGTGGAAAAAGTTATCCTATGAGAAAGGAATGCGAA
ACAAAATACTTTGATCACTGGATAGTAGGGTATAAACCATTAACCAAACGTTCAAGATATGGATTTATATTATATGGAGA
TGAGGAAACTCTTTTATGCACAGAAAAAAGAATAGAGGAGTTAGATGGAAAGTATGATGAAGAAAAATTAAGTGCTATAG
GAAACTTTTATTGTTTTCCATATTTAAATGCCATAGATGTTGCTAAAACACCACAATGGGTAAAGGATACTGTTTGGTAT
CAAATATTCCCAGATAGATTTTGCAATGGAGATAAATCAATAGATCCAGAAAATGTTGAGCCATGGGGGACAGAGCCTAC
TAGGGATAATTTTATGGGAGGAGATTTACAGGGAGTTTTAGATAAATTAGATTACTTATGCGATCTTGGAATTAATGGAC
TATATTTTTGTCCAGTATTTGAAGCTACTGAGAATCATAGGTATGAAACTATAGATTATTTTAAAGTAGATCCAGCACTT
GGTGGGAATGAAGTCTTTAAAAAACTTGTAAGTGAAGCTCACAAAAGAGGCATGAAAATAATGTTAGATGCAGTATTTAA
TCATATAGGTTATTTTTCACCAAAATGGCAAGATGTATTAAAGAATAATGAAAAATCAAGATATAAGGATTGGTTTTGTA
TAAAGAAGTTCCCAGTGCTTGAAAATGGCTTAGAAAATGTTGATGGAAATAATTTGAATTATGAAACCTTTGGAAGAATA
GCCACAATGCCTAAACTAAATACTGAAAACCCAGAGGTTGTAGAATATTTATTAAAAGTCGCTAAGTTCTGGGTTGAAGA
AATGGATATAGATGGTTGGAGACTTGATGTATGTAATGAAGTAGACCATGTATTTTGGAGAAAATTTAGGGAAGTAGTAA
AGGGAACTAATAAAGAAGTTTATATATTAGGAGAAGTTTGGCATGATGGACTTCCATGGCTTATGGGAGATCAGTTTGAT
TCAGTTATGAATTACCCTGTTACAGATGCAGTAAAAGAATATTTTTGTTTAAATCAATCTAATCCAGAAGATTTTAAATA
TATGATAGAAGCTAATAAGGTTAGCTATTTAAGACAAATAGGAGAAACAATATTTAACTTGTTAGATAGTCATGATACTC
CAAGAATATTAACTGTAGCTGGAGGAAATAAGGATAAGATGAAACTAGCTTATCTGTTTATGTTTACTCAAGCTGGCTCT
CCATGTATATATTATGGAGATGAAGTTGGTATGGAAGGAAATCAAGGAATGGGTATGGAATTCCATAGAAGATGTATGAT
TTGGGATGAAAATAAACAAGACAAAGATATGCTTAAGTTTATGAAACAAATAATAAAAATAAGAAAAGAAAATAAGGAAT
TAAATTTATTAGATAATAATTGGATAAGAGCTAATAAGGATGAAAATATACTTATTTATTCAAAGGAAAATATATTTATA
ATTATGAACAATTCAGAGAATGAAGAAAAGATATATTTACCTAAGGAGCTTAAAAATAATAAGATTAATGATTTATTTGA
AGAAAAAATTGAACTCTTAAAAGAAGATATAGACTTAAAGCCCTTTGCATTTAAAGTTTATAAAAAGCTTTAA

Upstream 100 bases:

>100_bases
GAGAAGATACACATATAAATATTTTTTTGATTTGTTTTGGTACCGTTACCAAAATTGTTTGTAATAATTTATGATTATAA
TATTATGGAGGGTTAAATTT

Downstream 100 bases:

>100_bases
TATTTAATAAATTAAAAAAAGAGTAAGAATTTTAGAATGTATTATATTTTATAATTTTTACTCTTTTTATTTATATTTAG
AAAGGATAAAATAACTATAG

Product: cyclomaltodextrinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MGKITREAIHHIAQSNYSYGYDNETLHLRVKTKKGEVNKVEIRIGDPYIWDEGGCDGGNMNATGGRWTGGKSYPMRKECE
TKYFDHWIVGYKPLTKRSRYGFILYGDEETLLCTEKRIEELDGKYDEEKLSAIGNFYCFPYLNAIDVAKTPQWVKDTVWY
QIFPDRFCNGDKSIDPENVEPWGTEPTRDNFMGGDLQGVLDKLDYLCDLGINGLYFCPVFEATENHRYETIDYFKVDPAL
GGNEVFKKLVSEAHKRGMKIMLDAVFNHIGYFSPKWQDVLKNNEKSRYKDWFCIKKFPVLENGLENVDGNNLNYETFGRI
ATMPKLNTENPEVVEYLLKVAKFWVEEMDIDGWRLDVCNEVDHVFWRKFREVVKGTNKEVYILGEVWHDGLPWLMGDQFD
SVMNYPVTDAVKEYFCLNQSNPEDFKYMIEANKVSYLRQIGETIFNLLDSHDTPRILTVAGGNKDKMKLAYLFMFTQAGS
PCIYYGDEVGMEGNQGMGMEFHRRCMIWDENKQDKDMLKFMKQIIKIRKENKELNLLDNNWIRANKDENILIYSKENIFI
IMNNSENEEKIYLPKELKNNKINDLFEEKIELLKEDIDLKPFAFKVYKKL

Sequences:

>Translated_610_residues
MGKITREAIHHIAQSNYSYGYDNETLHLRVKTKKGEVNKVEIRIGDPYIWDEGGCDGGNMNATGGRWTGGKSYPMRKECE
TKYFDHWIVGYKPLTKRSRYGFILYGDEETLLCTEKRIEELDGKYDEEKLSAIGNFYCFPYLNAIDVAKTPQWVKDTVWY
QIFPDRFCNGDKSIDPENVEPWGTEPTRDNFMGGDLQGVLDKLDYLCDLGINGLYFCPVFEATENHRYETIDYFKVDPAL
GGNEVFKKLVSEAHKRGMKIMLDAVFNHIGYFSPKWQDVLKNNEKSRYKDWFCIKKFPVLENGLENVDGNNLNYETFGRI
ATMPKLNTENPEVVEYLLKVAKFWVEEMDIDGWRLDVCNEVDHVFWRKFREVVKGTNKEVYILGEVWHDGLPWLMGDQFD
SVMNYPVTDAVKEYFCLNQSNPEDFKYMIEANKVSYLRQIGETIFNLLDSHDTPRILTVAGGNKDKMKLAYLFMFTQAGS
PCIYYGDEVGMEGNQGMGMEFHRRCMIWDENKQDKDMLKFMKQIIKIRKENKELNLLDNNWIRANKDENILIYSKENIFI
IMNNSENEEKIYLPKELKNNKINDLFEEKIELLKEDIDLKPFAFKVYKKL
>Mature_609_residues
GKITREAIHHIAQSNYSYGYDNETLHLRVKTKKGEVNKVEIRIGDPYIWDEGGCDGGNMNATGGRWTGGKSYPMRKECET
KYFDHWIVGYKPLTKRSRYGFILYGDEETLLCTEKRIEELDGKYDEEKLSAIGNFYCFPYLNAIDVAKTPQWVKDTVWYQ
IFPDRFCNGDKSIDPENVEPWGTEPTRDNFMGGDLQGVLDKLDYLCDLGINGLYFCPVFEATENHRYETIDYFKVDPALG
GNEVFKKLVSEAHKRGMKIMLDAVFNHIGYFSPKWQDVLKNNEKSRYKDWFCIKKFPVLENGLENVDGNNLNYETFGRIA
TMPKLNTENPEVVEYLLKVAKFWVEEMDIDGWRLDVCNEVDHVFWRKFREVVKGTNKEVYILGEVWHDGLPWLMGDQFDS
VMNYPVTDAVKEYFCLNQSNPEDFKYMIEANKVSYLRQIGETIFNLLDSHDTPRILTVAGGNKDKMKLAYLFMFTQAGSP
CIYYGDEVGMEGNQGMGMEFHRRCMIWDENKQDKDMLKFMKQIIKIRKENKELNLLDNNWIRANKDENILIYSKENIFII
MNNSENEEKIYLPKELKNNKINDLFEEKIELLKEDIDLKPFAFKVYKKL

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=397, Percent_Identity=24.9370277078086, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1786604, Length=492, Percent_Identity=30.0813008130081, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1790687, Length=394, Percent_Identity=26.1421319796954, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789995, Length=439, Percent_Identity=24.8291571753986, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322245, Length=230, Percent_Identity=26.9565217391304, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321726, Length=284, Percent_Identity=23.943661971831, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322241, Length=224, Percent_Identity=25.8928571428571, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322021, Length=224, Percent_Identity=25.8928571428571, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=226, Percent_Identity=26.5486725663717, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321731, Length=226, Percent_Identity=26.5486725663717, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324416, Length=224, Percent_Identity=25.8928571428571, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583747, Length=408, Percent_Identity=24.2647058823529, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583749, Length=408, Percent_Identity=24.2647058823529, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221330053, Length=412, Percent_Identity=23.0582524271845, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583745, Length=280, Percent_Identity=25.3571428571429, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24586599, Length=227, Percent_Identity=27.7533039647577, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 71489; Mature: 71358

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKITREAIHHIAQSNYSYGYDNETLHLRVKTKKGEVNKVEIRIGDPYIWDEGGCDGGNM
CCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCEEECCCCCCCCCC
NATGGRWTGGKSYPMRKECETKYFDHWIVGYKPLTKRSRYGFILYGDEETLLCTEKRIEE
CCCCCCCCCCCCCCCCHHHHHHHHCEEEECCCCCCCCCCEEEEEECCCCEEEEHHHHHHH
LDGKYDEEKLSAIGNFYCFPYLNAIDVAKTPQWVKDTVWYQIFPDRFCNGDKSIDPENVE
HCCCCCHHHHHHHHCEEEECCCCHHCCCCCCHHHHHHHEEEECCHHHCCCCCCCCCCCCC
PWGTEPTRDNFMGGDLQGVLDKLDYLCDLGINGLYFCPVFEATENHRYETIDYFKVDPAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEEECCCC
GGNEVFKKLVSEAHKRGMKIMLDAVFNHIGYFSPKWQDVLKNNEKSRYKDWFCIKKFPVL
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHCCEEEEECCCHH
ENGLENVDGNNLNYETFGRIATMPKLNTENPEVVEYLLKVAKFWVEEMDIDGWRLDVCNE
HCCCCCCCCCCCCHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
VDHVFWRKFREVVKGTNKEVYILGEVWHDGLPWLMGDQFDSVMNYPVTDAVKEYFCLNQS
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCC
NPEDFKYMIEANKVSYLRQIGETIFNLLDSHDTPRILTVAGGNKDKMKLAYLFMFTQAGS
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEEECCCC
PCIYYGDEVGMEGNQGMGMEFHRRCMIWDENKQDKDMLKFMKQIIKIRKENKELNLLDNN
CEEEECCCCCCCCCCCCCHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCC
WIRANKDENILIYSKENIFIIMNNSENEEKIYLPKELKNNKINDLFEEKIELLKEDIDLK
CEECCCCCEEEEEECCCEEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHCCCCC
PFAFKVYKKL
HHHHHHHHCC
>Mature Secondary Structure 
GKITREAIHHIAQSNYSYGYDNETLHLRVKTKKGEVNKVEIRIGDPYIWDEGGCDGGNM
CCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCEEECCCCCCCCCC
NATGGRWTGGKSYPMRKECETKYFDHWIVGYKPLTKRSRYGFILYGDEETLLCTEKRIEE
CCCCCCCCCCCCCCCCHHHHHHHHCEEEECCCCCCCCCCEEEEEECCCCEEEEHHHHHHH
LDGKYDEEKLSAIGNFYCFPYLNAIDVAKTPQWVKDTVWYQIFPDRFCNGDKSIDPENVE
HCCCCCHHHHHHHHCEEEECCCCHHCCCCCCHHHHHHHEEEECCHHHCCCCCCCCCCCCC
PWGTEPTRDNFMGGDLQGVLDKLDYLCDLGINGLYFCPVFEATENHRYETIDYFKVDPAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEEECCCC
GGNEVFKKLVSEAHKRGMKIMLDAVFNHIGYFSPKWQDVLKNNEKSRYKDWFCIKKFPVL
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHCCEEEEECCCHH
ENGLENVDGNNLNYETFGRIATMPKLNTENPEVVEYLLKVAKFWVEEMDIDGWRLDVCNE
HCCCCCCCCCCCCHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
VDHVFWRKFREVVKGTNKEVYILGEVWHDGLPWLMGDQFDSVMNYPVTDAVKEYFCLNQS
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCC
NPEDFKYMIEANKVSYLRQIGETIFNLLDSHDTPRILTVAGGNKDKMKLAYLFMFTQAGS
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEEECCCC
PCIYYGDEVGMEGNQGMGMEFHRRCMIWDENKQDKDMLKFMKQIIKIRKENKELNLLDNN
CEEEECCCCCCCCCCCCCHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCC
WIRANKDENILIYSKENIFIIMNNSENEEKIYLPKELKNNKINDLFEEKIELLKEDIDLK
CEECCCCCEEEEEECCCEEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHCCCCC
PFAFKVYKKL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]