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The map label for this gene is purD [H]

Identifier: 18309669

GI number: 18309669

Start: 855632

End: 856591

Strand: Direct

Name: purD [H]

Synonym: CPE0687

Alternate gene names: 18309669

Gene position: 855632-856591 (Clockwise)

Preceding gene: 18309668

Following gene: 18309670

Centisome position: 28.23

GC content: 30.63

Gene sequence:

>960_bases
ATGCTTGAAGGTAGTAAAAGTTTTTCTAAGGATTTTATGAAAAAGTATGGCGTTAAGACAGCTGATTACGAAGTTTTCTA
TGATTATGAAGAAGCTAAAAGTTATTTAGAAAATTGTAAGTATCCAAGAGTCATAAAGGCTGATGGATTGGCTGCTGGTA
AAGGTGTTGTAATATGTGAAAATAAAAATCAGGCAATAGAAACACTAGATTTATTTATGATAGAGGATGTATTTAATGGA
GCAGGAAAGAAAATAGTAGTAGAAGAATTTCTGCAAGGGGTTGAGGCATCAATACTTTCGGTTACAGATGGAAAAGTAAT
ATTACCATTTTTATCGGCTAAGGATCACAAACAAATATTTGATGGAAATAAGGGTAAAAATACAGGGGGAATGGGAGTTG
TTTCTCCTAATAAATATGTTACTGAAGAGGTTTTAGCTGGTTTTTATGAAAATATATTAGAGAAAACTTTAATAGGTATA
AGAGAAGAAGGCTTTGACTTTAAAGGAGTTATTTTTTTTGGAATAATGGTAACAGAAGAAGGGGCTAAACTTTTAGAATA
TAATGTTAGGATGGGAGATCCAGAAACACAAAGTGTTTTATCTTTAATGGAAAGTGATTTAGTAGACATAATACTAAATG
CACTAGATGAAAAATTAAATGAAACAACTATTAAATGGAATGAAGGATATTGCGTTAATGTTGTTTTATCATCAAAGGGA
TATCCAGAGGCTTATGAAAAAGGTTATGAAATAAGTATAGATGAAAGTCTTAAGGGAGAATTTTTTATAGCTGGAGCAAA
AAAAGAAGGGGATACCTTAGTAACTAGTGGAGGAAGAGTACTTTCTGTAGTAGGTAAAGGAAAAACTTTAGAAGAAGCTA
AAGAAAATGCATATAAAAATATAGAAAAAGTTAATTTTGAGGGAATGTATTTTAGAAAAGACATAGGTACTGCTAAATAG

Upstream 100 bases:

>100_bases
TTTAACTGTTGTAGGACCAGAAGAACCACTTATTAATGGTATAGTTGATGCTTTTAAAGAGGAAGGATTAAAAATATTTG
GACCTGATAAAGCTGGAGCT

Downstream 100 bases:

>100_bases
CCCTTAGAAATTAGCAAATTTGAATATATTATAATCATAAGTTATATATTTAGTATTTAATATTTTAGATAGAATTTAAA
CAATACAGTTTTAATTTATA

Product: phosphoribosylamine-glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLEGSKSFSKDFMKKYGVKTADYEVFYDYEEAKSYLENCKYPRVIKADGLAAGKGVVICENKNQAIETLDLFMIEDVFNG
AGKKIVVEEFLQGVEASILSVTDGKVILPFLSAKDHKQIFDGNKGKNTGGMGVVSPNKYVTEEVLAGFYENILEKTLIGI
REEGFDFKGVIFFGIMVTEEGAKLLEYNVRMGDPETQSVLSLMESDLVDIILNALDEKLNETTIKWNEGYCVNVVLSSKG
YPEAYEKGYEISIDESLKGEFFIAGAKKEGDTLVTSGGRVLSVVGKGKTLEEAKENAYKNIEKVNFEGMYFRKDIGTAK

Sequences:

>Translated_319_residues
MLEGSKSFSKDFMKKYGVKTADYEVFYDYEEAKSYLENCKYPRVIKADGLAAGKGVVICENKNQAIETLDLFMIEDVFNG
AGKKIVVEEFLQGVEASILSVTDGKVILPFLSAKDHKQIFDGNKGKNTGGMGVVSPNKYVTEEVLAGFYENILEKTLIGI
REEGFDFKGVIFFGIMVTEEGAKLLEYNVRMGDPETQSVLSLMESDLVDIILNALDEKLNETTIKWNEGYCVNVVLSSKG
YPEAYEKGYEISIDESLKGEFFIAGAKKEGDTLVTSGGRVLSVVGKGKTLEEAKENAYKNIEKVNFEGMYFRKDIGTAK
>Mature_319_residues
MLEGSKSFSKDFMKKYGVKTADYEVFYDYEEAKSYLENCKYPRVIKADGLAAGKGVVICENKNQAIETLDLFMIEDVFNG
AGKKIVVEEFLQGVEASILSVTDGKVILPFLSAKDHKQIFDGNKGKNTGGMGVVSPNKYVTEEVLAGFYENILEKTLIGI
REEGFDFKGVIFFGIMVTEEGAKLLEYNVRMGDPETQSVLSLMESDLVDIILNALDEKLNETTIKWNEGYCVNVVLSSKG
YPEAYEKGYEISIDESLKGEFFIAGAKKEGDTLVTSGGRVLSVVGKGKTLEEAKENAYKNIEKVNFEGMYFRKDIGTAK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=320, Percent_Identity=41.5625, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI4503915, Length=320, Percent_Identity=41.5625, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI209869995, Length=320, Percent_Identity=41.5625, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI209869993, Length=320, Percent_Identity=41.5625, Blast_Score=251, Evalue=5e-67,
Organism=Escherichia coli, GI1790438, Length=320, Percent_Identity=40.9375, Blast_Score=253, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17567511, Length=323, Percent_Identity=41.4860681114551, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6321203, Length=331, Percent_Identity=40.785498489426, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI62471619, Length=325, Percent_Identity=39.0769230769231, Blast_Score=217, Evalue=8e-57,
Organism=Drosophila melanogaster, GI24582400, Length=326, Percent_Identity=38.9570552147239, Blast_Score=211, Evalue=7e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 35385; Mature: 35385

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEGSKSFSKDFMKKYGVKTADYEVFYDYEEAKSYLENCKYPRVIKADGLAAGKGVVICE
CCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEC
NKNQAIETLDLFMIEDVFNGAGKKIVVEEFLQGVEASILSVTDGKVILPFLSAKDHKQIF
CCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHEEEEECCCCEEEEECCCCCCHHHH
DGNKGKNTGGMGVVSPNKYVTEEVLAGFYENILEKTLIGIREEGFDFKGVIFFGIMVTEE
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECC
GAKLLEYNVRMGDPETQSVLSLMESDLVDIILNALDEKLNETTIKWNEGYCVNVVLSSKG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEEECCC
YPEAYEKGYEISIDESLKGEFFIAGAKKEGDTLVTSGGRVLSVVGKGKTLEEAKENAYKN
CCHHHHCCCEEEECCCCCCCEEEEECCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHH
IEKVNFEGMYFRKDIGTAK
HHHCCCCCEEEECCCCCCC
>Mature Secondary Structure
MLEGSKSFSKDFMKKYGVKTADYEVFYDYEEAKSYLENCKYPRVIKADGLAAGKGVVICE
CCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEC
NKNQAIETLDLFMIEDVFNGAGKKIVVEEFLQGVEASILSVTDGKVILPFLSAKDHKQIF
CCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHEEEEECCCCEEEEECCCCCCHHHH
DGNKGKNTGGMGVVSPNKYVTEEVLAGFYENILEKTLIGIREEGFDFKGVIFFGIMVTEE
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECC
GAKLLEYNVRMGDPETQSVLSLMESDLVDIILNALDEKLNETTIKWNEGYCVNVVLSSKG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEEECCC
YPEAYEKGYEISIDESLKGEFFIAGAKKEGDTLVTSGGRVLSVVGKGKTLEEAKENAYKN
CCHHHHCCCEEEECCCCCCCEEEEECCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHH
IEKVNFEGMYFRKDIGTAK
HHHCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]