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The map label for this gene is thiC

Identifier: 18309661

GI number: 18309661

Start: 843791

End: 845101

Strand: Direct

Name: thiC

Synonym: CPE0679

Alternate gene names: 18309661

Gene position: 843791-845101 (Clockwise)

Preceding gene: 18309660

Following gene: 18309662

Centisome position: 27.83

GC content: 31.58

Gene sequence:

>1311_bases
ATGAATTATACAACTCAAATGGATGCTGCTAAAAAAGGAATAATAACAAAGGAAATGCAAGTAGTTTCAGAAAAAGAAGG
AATTAATATTGAAACTTTAATGAATTTAATGGCTGAAGGAAAAATTGTAATACCAGCTAATAAAAATCATAAAAGTATAA
GTGCAGAGGGTGTTGGACAAGGGTTAAGAACTAAAATAAATGTTAACCTAGGAATTTCAAAGGATTGTGCCAATATAGAA
TTAGAGTTAGAAAAAGTTAAAAAGGCAATAGATATGAATGCAGAATCTATAATGGATTTAAGTAATTATGGTAAAACTTA
TGATTTTAGAAAAAGACTTGTAGAAGTTTCTACGGCTATGATAGGAACTGTACCAATGTATGATGTAGTAGGTTTCTATG
ATAAAGAGCTTAAAGATATAACTGTTGATGAATTTTTTGAAGTTGTAGAAAAACATGCAAAGGATGGAGTTGACTTTGTT
ACTATACATGCTGGATTAAATAGAGAAACAATTGAAACTTTTAGAAGAAATAAAAGACTTACTAATATAGTTTCTAGAGG
AGGATCTCTTCTTTTTGCATGGATGGAATTAAATAATAGAGAAAATCCTTTCTATGAATATTTTGATAGATTATTAGATA
TATGTGAAAAGTATGATTTAACTTTAAGTTTAGGGGATGCTTGTAGACCAGGTTCAATAGCTGATGCAACTGATGCTGTA
CAAATCAAAGAATTAATTACTCTTGGAGAGCTAACAAAAAGAGCTTGGGAAAGAAATGTACAAGTAATAATAGAGGGTCC
AGGGCATATGGCAATGAATGAAATAGAAGCTAATGTTTTATTAGAGAAAAAATTATGCCATGGAGCACCATTTTATGTTT
TAGGACCAATAGTAACTGATATTGCACCAGGATATGATCATATAACAAGTGCTATAGGAGGGGCTATGGCAGCTTCTTAT
GGAGCAGATTTTCTTTGTTATGTAACACCAGCAGAACATTTAAGACTTCCTAATTTAGAGGATGTAAGGGAAGGAATAGT
TGCCACAAAGATAGCGGCTCATGCAGCTGACATAGCAAAAGGAATTTCTGGGGCAAGGGACATAGATAATAAAATGAGTG
ATGCTAGGAAAAGACTAGATTGGGACGAGATGTTTTCTTTAGCTATAGATAGTGAAAAAGCCATTAGATATAGAAAAGAA
TCTACTCCTGAACATAAAGATAGTTGTACAATGTGTGGAAAAATGTGCTCTATAAGAAATATGAATAAGATTCTAGAAGG
AAAGGATATAAATCTTTTAAGAGAAGACTAA

Upstream 100 bases:

>100_bases
ATTAAGCTTGCCTTAGGGCAAGCTTTTATTTTTTATAAAAAAATAGGATCCTATATTAATTTAACTGCGCAGTTAAAAAA
TTAAAATAAGGAGAACTAAT

Downstream 100 bases:

>100_bases
AAATATTAGAGATATATTAAAAAATATTATTCGAGACTTTTAATATATCTCTATTTTTATTCTTAAATACGAACTTTAAT
TAATAAAAACAATATAAATA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNYTTQMDAAKKGIITKEMQVVSEKEGINIETLMNLMAEGKIVIPANKNHKSISAEGVGQGLRTKINVNLGISKDCANIE
LELEKVKKAIDMNAESIMDLSNYGKTYDFRKRLVEVSTAMIGTVPMYDVVGFYDKELKDITVDEFFEVVEKHAKDGVDFV
TIHAGLNRETIETFRRNKRLTNIVSRGGSLLFAWMELNNRENPFYEYFDRLLDICEKYDLTLSLGDACRPGSIADATDAV
QIKELITLGELTKRAWERNVQVIIEGPGHMAMNEIEANVLLEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAMAASY
GADFLCYVTPAEHLRLPNLEDVREGIVATKIAAHAADIAKGISGARDIDNKMSDARKRLDWDEMFSLAIDSEKAIRYRKE
STPEHKDSCTMCGKMCSIRNMNKILEGKDINLLRED

Sequences:

>Translated_436_residues
MNYTTQMDAAKKGIITKEMQVVSEKEGINIETLMNLMAEGKIVIPANKNHKSISAEGVGQGLRTKINVNLGISKDCANIE
LELEKVKKAIDMNAESIMDLSNYGKTYDFRKRLVEVSTAMIGTVPMYDVVGFYDKELKDITVDEFFEVVEKHAKDGVDFV
TIHAGLNRETIETFRRNKRLTNIVSRGGSLLFAWMELNNRENPFYEYFDRLLDICEKYDLTLSLGDACRPGSIADATDAV
QIKELITLGELTKRAWERNVQVIIEGPGHMAMNEIEANVLLEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAMAASY
GADFLCYVTPAEHLRLPNLEDVREGIVATKIAAHAADIAKGISGARDIDNKMSDARKRLDWDEMFSLAIDSEKAIRYRKE
STPEHKDSCTMCGKMCSIRNMNKILEGKDINLLRED
>Mature_436_residues
MNYTTQMDAAKKGIITKEMQVVSEKEGINIETLMNLMAEGKIVIPANKNHKSISAEGVGQGLRTKINVNLGISKDCANIE
LELEKVKKAIDMNAESIMDLSNYGKTYDFRKRLVEVSTAMIGTVPMYDVVGFYDKELKDITVDEFFEVVEKHAKDGVDFV
TIHAGLNRETIETFRRNKRLTNIVSRGGSLLFAWMELNNRENPFYEYFDRLLDICEKYDLTLSLGDACRPGSIADATDAV
QIKELITLGELTKRAWERNVQVIIEGPGHMAMNEIEANVLLEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAMAASY
GADFLCYVTPAEHLRLPNLEDVREGIVATKIAAHAADIAKGISGARDIDNKMSDARKRLDWDEMFSLAIDSEKAIRYRKE
STPEHKDSCTMCGKMCSIRNMNKILEGKDINLLRED

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=447, Percent_Identity=42.7293064876958, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_CLOP1 (Q0TTB7)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_695123.1
- STRING:   Q0TTB7
- GeneID:   4201374
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_0670
- TIGR:   CPF_0670
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- BioCyc:   CPER195103:CPF_0670-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 48719; Mature: 48719

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 98-98 BINDING 127-127 BINDING 163-163 BINDING 265-265 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTTQMDAAKKGIITKEMQVVSEKEGINIETLMNLMAEGKIVIPANKNHKSISAEGVGQ
CCCCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHCCC
GLRTKINVNLGISKDCANIELELEKVKKAIDMNAESIMDLSNYGKTYDFRKRLVEVSTAM
CCEEEEEEEECCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGTVPMYDVVGFYDKELKDITVDEFFEVVEKHAKDGVDFVTIHAGLNRETIETFRRNKRL
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHH
TNIVSRGGSLLFAWMELNNRENPFYEYFDRLLDICEKYDLTLSLGDACRPGSIADATDAV
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHH
QIKELITLGELTKRAWERNVQVIIEGPGHMAMNEIEANVLLEKKLCHGAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGAMAASYGADFLCYVTPAEHLRLPNLEDVREGIVATKIAAHAADIAK
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GISGARDIDNKMSDARKRLDWDEMFSLAIDSEKAIRYRKESTPEHKDSCTMCGKMCSIRN
HCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
MNKILEGKDINLLRED
HHHHHCCCCCCEEECC
>Mature Secondary Structure
MNYTTQMDAAKKGIITKEMQVVSEKEGINIETLMNLMAEGKIVIPANKNHKSISAEGVGQ
CCCCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHCCC
GLRTKINVNLGISKDCANIELELEKVKKAIDMNAESIMDLSNYGKTYDFRKRLVEVSTAM
CCEEEEEEEECCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGTVPMYDVVGFYDKELKDITVDEFFEVVEKHAKDGVDFVTIHAGLNRETIETFRRNKRL
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHH
TNIVSRGGSLLFAWMELNNRENPFYEYFDRLLDICEKYDLTLSLGDACRPGSIADATDAV
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHH
QIKELITLGELTKRAWERNVQVIIEGPGHMAMNEIEANVLLEKKLCHGAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGAMAASYGADFLCYVTPAEHLRLPNLEDVREGIVATKIAAHAADIAK
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GISGARDIDNKMSDARKRLDWDEMFSLAIDSEKAIRYRKESTPEHKDSCTMCGKMCSIRN
HCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
MNKILEGKDINLLRED
HHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA