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The map label for this gene is tyrS [H]

Identifier: 18309634

GI number: 18309634

Start: 814422

End: 815642

Strand: Direct

Name: tyrS [H]

Synonym: CPE0652

Alternate gene names: 18309634

Gene position: 814422-815642 (Clockwise)

Preceding gene: 18309633

Following gene: 18309635

Centisome position: 26.87

GC content: 32.92

Gene sequence:

>1221_bases
ATGGCTAATGTATTAGATACGTTAATGGAACGTGGGTACATAAAACAGTTTACTCATGAAGCTGAAACAAGAGAACTTTT
AGAGAAGGAAAAAGTTACTTTTTATATAGGATTTGACCCAACAGCTGATAGTTTACATGTAGGACACTTCATTGCAATGA
TGTTTATGGCTCATATGCAAAAGGCTGGACATAGACCAATAGCTTTAATTGGTGGAGGTACAGCTACAATTGGAGATCCA
AGTGGTAAGACTGATATGAGAAAGATGATGACAAATGAGACTATAGCTCATAATGTTGCTTGTATCAAAAAGCAAATGGA
GAAATTCATAGATTTTTCAGATGATAAAGCTATACTTGTTAACAATGCTGATTGGTTATTAAACCAAAACTATGTTGAAT
TCTTAAGAGAGGTTGGAGTTCACTTCTCAGTTAACAGAATGCTTTCAGCAGAATGTTTTAAACAAAGACTTGAAAGAGGT
CTTTCATTCTTAGAATTTAACTACATGTTAATGCAAGGATATGACTTCTATGTTTTAAATAAAAAATACAACTGTAAAAT
GGAGCTAGGTGGAGATGACCAATGGTCAAACATGATAGCTGGTGTTGAACTTGTAAGAAAGAAATCACAAGAGAGTGCAT
ATGCTATGACTTGCACATTATTAACTAACTCAGAAGGACAAAAAATGGGTAAAACAGTTAATGGTGCATTATGGTTAGAT
CCAGAGAAAACTTCTCCATACGAGTTCTACCAATACTGGAGAAATGTTAATGATGCAGACGTTGAAAAATGCTTAAAACT
ATTAACATTTATTCCAATGGATGAAGTTAGAAGACTTTCAAGTTTAGAAGGTTCTCAAATAAATGAAGCTAAAAAAGTTT
TAGCATTTGAGGTAACTAAACTTGTACATGGTGAAGAGGAAGCTACAAAGGCTCAACAAGCTGCTGAAGCTTTATTTGGA
AAAGGTGGAGATATGAGTAATGTTCCAACTTATGAAATGGGAAAAGATAAGTTAGGTGCTGAACTTTTAGATATTTTAGT
TGAAGCTGAAATAGTTCCATCAAAAGCTGAAGGGAAAAGACTTGTTAAGCAAGGTGGATTATCATTAAATGGAGAAAAAG
TTGCTGATTTTAAAAAGACATTAGAAGAAGCTGACTTTGAAAATGGTGAAGTTTTAATTAAAAGAGGTAAGAAAAACTAC
AATAAAATAGTTTTAGCATAA

Upstream 100 bases:

>100_bases
TGAGAATTAATATAATAAAGGATATACTATATACTATATGATTGCTAAATATAGCACCAGCAAGTAAGCTGGATTAGAAT
TTTTTTGGAGGGATAAACAA

Downstream 100 bases:

>100_bases
TTCTTTTTTAGAAGAGTGTATTAGAATATATTAGCTAATTATTATTAGGGGTAATATATTCTATTTACACTCTTTTATTT
ATATGTGATTTTAATTAGAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MANVLDTLMERGYIKQFTHEAETRELLEKEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQKAGHRPIALIGGGTATIGDP
SGKTDMRKMMTNETIAHNVACIKKQMEKFIDFSDDKAILVNNADWLLNQNYVEFLREVGVHFSVNRMLSAECFKQRLERG
LSFLEFNYMLMQGYDFYVLNKKYNCKMELGGDDQWSNMIAGVELVRKKSQESAYAMTCTLLTNSEGQKMGKTVNGALWLD
PEKTSPYEFYQYWRNVNDADVEKCLKLLTFIPMDEVRRLSSLEGSQINEAKKVLAFEVTKLVHGEEEATKAQQAAEALFG
KGGDMSNVPTYEMGKDKLGAELLDILVEAEIVPSKAEGKRLVKQGGLSLNGEKVADFKKTLEEADFENGEVLIKRGKKNY
NKIVLA

Sequences:

>Translated_406_residues
MANVLDTLMERGYIKQFTHEAETRELLEKEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQKAGHRPIALIGGGTATIGDP
SGKTDMRKMMTNETIAHNVACIKKQMEKFIDFSDDKAILVNNADWLLNQNYVEFLREVGVHFSVNRMLSAECFKQRLERG
LSFLEFNYMLMQGYDFYVLNKKYNCKMELGGDDQWSNMIAGVELVRKKSQESAYAMTCTLLTNSEGQKMGKTVNGALWLD
PEKTSPYEFYQYWRNVNDADVEKCLKLLTFIPMDEVRRLSSLEGSQINEAKKVLAFEVTKLVHGEEEATKAQQAAEALFG
KGGDMSNVPTYEMGKDKLGAELLDILVEAEIVPSKAEGKRLVKQGGLSLNGEKVADFKKTLEEADFENGEVLIKRGKKNY
NKIVLA
>Mature_405_residues
ANVLDTLMERGYIKQFTHEAETRELLEKEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQKAGHRPIALIGGGTATIGDPS
GKTDMRKMMTNETIAHNVACIKKQMEKFIDFSDDKAILVNNADWLLNQNYVEFLREVGVHFSVNRMLSAECFKQRLERGL
SFLEFNYMLMQGYDFYVLNKKYNCKMELGGDDQWSNMIAGVELVRKKSQESAYAMTCTLLTNSEGQKMGKTVNGALWLDP
EKTSPYEFYQYWRNVNDADVEKCLKLLTFIPMDEVRRLSSLEGSQINEAKKVLAFEVTKLVHGEEEATKAQQAAEALFGK
GGDMSNVPTYEMGKDKLGAELLDILVEAEIVPSKAEGKRLVKQGGLSLNGEKVADFKKTLEEADFENGEVLIKRGKKNYN
KIVLA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=428, Percent_Identity=35.981308411215, Blast_Score=258, Evalue=1e-68,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=42.9245283018868, Blast_Score=354, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI25145802, Length=426, Percent_Identity=34.5070422535211, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI25145805, Length=325, Percent_Identity=33.5384615384615, Blast_Score=166, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6325160, Length=403, Percent_Identity=31.2655086848635, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI20130383, Length=433, Percent_Identity=33.9491916859122, Blast_Score=264, Evalue=7e-71,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45881; Mature: 45750

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVLDTLMERGYIKQFTHEAETRELLEKEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQ
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
KAGHRPIALIGGGTATIGDPSGKTDMRKMMTNETIAHNVACIKKQMEKFIDFSDDKAILV
HCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
NNADWLLNQNYVEFLREVGVHFSVNRMLSAECFKQRLERGLSFLEFNYMLMQGYDFYVLN
ECCHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEC
KKYNCKMELGGDDQWSNMIAGVELVRKKSQESAYAMTCTLLTNSEGQKMGKTVNGALWLD
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHCCCCCCEEEEC
PEKTSPYEFYQYWRNVNDADVEKCLKLLTFIPMDEVRRLSSLEGSQINEAKKVLAFEVTK
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVHGEEEATKAQQAAEALFGKGGDMSNVPTYEMGKDKLGAELLDILVEAEIVPSKAEGKR
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
LVKQGGLSLNGEKVADFKKTLEEADFENGEVLIKRGKKNYNKIVLA
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEC
>Mature Secondary Structure 
ANVLDTLMERGYIKQFTHEAETRELLEKEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQ
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
KAGHRPIALIGGGTATIGDPSGKTDMRKMMTNETIAHNVACIKKQMEKFIDFSDDKAILV
HCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
NNADWLLNQNYVEFLREVGVHFSVNRMLSAECFKQRLERGLSFLEFNYMLMQGYDFYVLN
ECCHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEC
KKYNCKMELGGDDQWSNMIAGVELVRKKSQESAYAMTCTLLTNSEGQKMGKTVNGALWLD
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHCCCCCCEEEEC
PEKTSPYEFYQYWRNVNDADVEKCLKLLTFIPMDEVRRLSSLEGSQINEAKKVLAFEVTK
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVHGEEEATKAQQAAEALFGKGGDMSNVPTYEMGKDKLGAELLDILVEAEIVPSKAEGKR
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
LVKQGGLSLNGEKVADFKKTLEEADFENGEVLIKRGKKNYNKIVLA
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA