Click here to switch to the map view.

The map label for this gene is ttcA

Identifier: 182682182

GI number: 182682182

Start: 1839250

End: 1840149

Strand: Direct

Name: ttcA

Synonym: XfasM23_1661

Alternate gene names: 182682182

Gene position: 1839250-1840149 (Clockwise)

Preceding gene: 182682181

Following gene: 182682183

Centisome position: 72.53

GC content: 53.56

Gene sequence:

>900_bases
ATGGATGCGACCTTCCCCTCTCAACACAACATCACCGCCAGACCTGCTCCAGGCAGGATACGCCGTGAACAATGCAAGCT
TGCCAAGCGCCTACGCCGTCAAGTGGGTCAAGCCATCGCTGACTTCGGCATGATCGAAGCCAACGATAAGATCATGGTCT
GCTTGTCCGGCGGCAAAGATAGCTACACCCTGCTGGACATGTTGTTGCAACTGCGTGCAAAAGCCCCGGTGCCGTTCGAA
CTGACCGCAGTCAATTTGGACCAAAAGCAACCCGGCTTTCCCAAACACGTCCTGCCAGAATACTTAAGCAGCATTGGCGT
GCCCTACCATATCATTGAGCAAGACACCTATTCGGTCGTGACTCGTGTCGTCCCCGAAGGTAAAACCCTATGCGCGCTCT
GCTCGCGGATGCGCCGCGGAGCACTGTACGCCTATGCGGAAACACAAGGCTTCACCAAAATCGCGTTGGGCCACCACCGC
GACGATATGGTTGCAACATTCTTTATGAATCTTTTTCACCACGCCAAGCTATCAGGGATGCCACCGAAGCTACGCAGTGA
TAATGGCAAACATGTCGTGATTCGCCCACTGGCTTACGTGAGCGAAACCGACATCATTGCTTACGCCGATGCACGTGAAT
TCCCGATCATTCCCTGCAATTTATGTGGCAGCCAAGAAAACCTGCAACGCAAACAAGTGGGAGTGATGCTCAAAGCATGG
GAAAAAGAATATCCAGGCCGAATAGAACAAATCGCACGTGCACTGGGCAATATTCGCCCCTCACAATTAGCCGACCAATC
CCTATTCGACTTCCTGGCATTGGGTCGACATAGCAACACCCCATTGCCTAATGCACACGCTTGGCTGGCTGGGGACCTCG
CCAACGACACGGCTCCATAG

Upstream 100 bases:

>100_bases
GGCAACTCAAACCGACATTCGATCACCTTCCACATAGAAAGTAACTGTAAGGCACTCATCAGTGAGCGCGCCGACCGGGG
CCAGCTACAATGAGCGCCCT

Downstream 100 bases:

>100_bases
AGCGCCTATTCCCCTTTCCATTTCCATCCGGACACACAATGTTCTTTCGTAATCTGACCTTGTTTCGCTTCCCAACGTCA
CTAGATTTCTCACAGATCGA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDATFPSQHNITARPAPGRIRREQCKLAKRLRRQVGQAIADFGMIEANDKIMVCLSGGKDSYTLLDMLLQLRAKAPVPFE
LTAVNLDQKQPGFPKHVLPEYLSSIGVPYHIIEQDTYSVVTRVVPEGKTLCALCSRMRRGALYAYAETQGFTKIALGHHR
DDMVATFFMNLFHHAKLSGMPPKLRSDNGKHVVIRPLAYVSETDIIAYADAREFPIIPCNLCGSQENLQRKQVGVMLKAW
EKEYPGRIEQIARALGNIRPSQLADQSLFDFLALGRHSNTPLPNAHAWLAGDLANDTAP

Sequences:

>Translated_299_residues
MDATFPSQHNITARPAPGRIRREQCKLAKRLRRQVGQAIADFGMIEANDKIMVCLSGGKDSYTLLDMLLQLRAKAPVPFE
LTAVNLDQKQPGFPKHVLPEYLSSIGVPYHIIEQDTYSVVTRVVPEGKTLCALCSRMRRGALYAYAETQGFTKIALGHHR
DDMVATFFMNLFHHAKLSGMPPKLRSDNGKHVVIRPLAYVSETDIIAYADAREFPIIPCNLCGSQENLQRKQVGVMLKAW
EKEYPGRIEQIARALGNIRPSQLADQSLFDFLALGRHSNTPLPNAHAWLAGDLANDTAP
>Mature_299_residues
MDATFPSQHNITARPAPGRIRREQCKLAKRLRRQVGQAIADFGMIEANDKIMVCLSGGKDSYTLLDMLLQLRAKAPVPFE
LTAVNLDQKQPGFPKHVLPEYLSSIGVPYHIIEQDTYSVVTRVVPEGKTLCALCSRMRRGALYAYAETQGFTKIALGHHR
DDMVATFFMNLFHHAKLSGMPPKLRSDNGKHVVIRPLAYVSETDIIAYADAREFPIIPCNLCGSQENLQRKQVGVMLKAW
EKEYPGRIEQIARALGNIRPSQLADQSLFDFLALGRHSNTPLPNAHAWLAGDLANDTAP

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=271, Percent_Identity=60.5166051660517, Blast_Score=348, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI193211090, Length=243, Percent_Identity=32.0987654320988, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24651972, Length=249, Percent_Identity=26.5060240963855, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_XYLF2 (B2I7H1)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830342.1
- ProteinModelPortal:   B2I7H1
- GeneID:   6202250
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1661
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- ProtClustDB:   PRK10696
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 33330; Mature: 33330

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDATFPSQHNITARPAPGRIRREQCKLAKRLRRQVGQAIADFGMIEANDKIMVCLSGGKD
CCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEEECCCC
SYTLLDMLLQLRAKAPVPFELTAVNLDQKQPGFPKHVLPEYLSSIGVPYHIIEQDTYSVV
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHH
TRVVPEGKTLCALCSRMRRGALYAYAETQGFTKIALGHHRDDMVATFFMNLFHHAKLSGM
HHHCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC
PPKLRSDNGKHVVIRPLAYVSETDIIAYADAREFPIIPCNLCGSQENLQRKQVGVMLKAW
CCCCCCCCCCEEEEEEHHHHCCCCEEEEECCCCCCEEEHHCCCCHHHHHHHHHHHHHHHH
EKEYPGRIEQIARALGNIRPSQLADQSLFDFLALGRHSNTPLPNAHAWLAGDLANDTAP
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MDATFPSQHNITARPAPGRIRREQCKLAKRLRRQVGQAIADFGMIEANDKIMVCLSGGKD
CCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEEECCCC
SYTLLDMLLQLRAKAPVPFELTAVNLDQKQPGFPKHVLPEYLSSIGVPYHIIEQDTYSVV
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHH
TRVVPEGKTLCALCSRMRRGALYAYAETQGFTKIALGHHRDDMVATFFMNLFHHAKLSGM
HHHCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC
PPKLRSDNGKHVVIRPLAYVSETDIIAYADAREFPIIPCNLCGSQENLQRKQVGVMLKAW
CCCCCCCCCCEEEEEEHHHHCCCCEEEEECCCCCCEEEHHCCCCHHHHHHHHHHHHHHHH
EKEYPGRIEQIARALGNIRPSQLADQSLFDFLALGRHSNTPLPNAHAWLAGDLANDTAP
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA