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The map label for this gene is pyrB

Identifier: 182681891

GI number: 182681891

Start: 1500276

End: 1501214

Strand: Reverse

Name: pyrB

Synonym: XfasM23_1359

Alternate gene names: 182681891

Gene position: 1501214-1500276 (Counterclockwise)

Preceding gene: 182681892

Following gene: 182681890

Centisome position: 59.2

GC content: 55.17

Gene sequence:

>939_bases
ATGCAATTTGATTCAAATGGACGTTTGCGTCACCTGCTCACTTTGGAAGGACTGTCGCGTCACACACTGCTGCAGTTACT
TGACTGTGCTGCCCAGAACTGTGCACTCGGGGTTGACCCTATGGGCAAGCGCAACGTGTTAGCTGGTATGGCAGTATGCA
CACTCTTTTTTGAACCATCCACGCGTACCCGTCATTCATTCCACCTGGCCGCGCAGCGTTTGGGAGCGGATGTATTAAAT
TTCGATGCTTCGATGTCGTCTACGAGCAAGGGTGAGAGTGCCTGCGACACATTGAAGAATTTGGAGGCAATGGGGGTGCG
CGGTTTTATAGTGCGCCATCCTGAAGAGATCGTCATCGCACAGTTGGCTGCAGTCGTTGGTGAGGGTACCGTGCTGATCA
ATGCCGGTGCTGGCCGTAGTACCCATCCGACGCAGGGGCTGCTGGATATGCTGACCTTATGTCAGGCCAAGGGCAACGAT
TTTTCCAAGCTAAAGCTACTCTTTGTCGGTGACATCAAACATTCACGCGTTGCACGCTCCAACCTGCATGCGTTGCGCAC
CCTGGGTGCAGGACAGATCCGGGTGTGCGGTCCGACTGCATTATTGCCCCATGACGGCCTCCTCTCTGGTTGTGTTGTCA
GTCAAGATTTTGATGCCATGCTTGAAGGTGTGGATGTACTGATGATGCTGCGTCTACAGCGTGAGCGTATGGAGGAAGGA
TTGGTGCCCTCGTTGGAGCACTACCATGCCAACTATGGTTTGACCGCAGCGCGCCTGGCGCGTGCAGCTCCGGATGCGGT
GGTATTACATCCTGGGCCAATCAACCGCGGCGTGGAGATTACCGATGAGGTGGCCGATGGCCCGCAGTCATGGATCCTGC
GTCAAGCCAGCAATGGCGTGATGGTCCGTATGGCCGTGCTGGAGACGTTACTGGGTTGA

Upstream 100 bases:

>100_bases
GATGCGATAGCGGCCGCAGTCATCCTTGAGCGTTGGCTTGCCGATCCTATGCAGGCGACATCACTTCCTTGATTTTTTGA
ACCTTAAAGCGATCACTCTT

Downstream 100 bases:

>100_bases
GCCTGAAAGTATTGCGTAGTTTCGTCGTGTGTGAGGTTGAGATAGCCAATCTCTAGGCGTTGTGAGCATGGTAAGCCGCC
TGTGTCCGCGCAACCGTGTC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MQFDSNGRLRHLLTLEGLSRHTLLQLLDCAAQNCALGVDPMGKRNVLAGMAVCTLFFEPSTRTRHSFHLAAQRLGADVLN
FDASMSSTSKGESACDTLKNLEAMGVRGFIVRHPEEIVIAQLAAVVGEGTVLINAGAGRSTHPTQGLLDMLTLCQAKGND
FSKLKLLFVGDIKHSRVARSNLHALRTLGAGQIRVCGPTALLPHDGLLSGCVVSQDFDAMLEGVDVLMMLRLQRERMEEG
LVPSLEHYHANYGLTAARLARAAPDAVVLHPGPINRGVEITDEVADGPQSWILRQASNGVMVRMAVLETLLG

Sequences:

>Translated_312_residues
MQFDSNGRLRHLLTLEGLSRHTLLQLLDCAAQNCALGVDPMGKRNVLAGMAVCTLFFEPSTRTRHSFHLAAQRLGADVLN
FDASMSSTSKGESACDTLKNLEAMGVRGFIVRHPEEIVIAQLAAVVGEGTVLINAGAGRSTHPTQGLLDMLTLCQAKGND
FSKLKLLFVGDIKHSRVARSNLHALRTLGAGQIRVCGPTALLPHDGLLSGCVVSQDFDAMLEGVDVLMMLRLQRERMEEG
LVPSLEHYHANYGLTAARLARAAPDAVVLHPGPINRGVEITDEVADGPQSWILRQASNGVMVRMAVLETLLG
>Mature_312_residues
MQFDSNGRLRHLLTLEGLSRHTLLQLLDCAAQNCALGVDPMGKRNVLAGMAVCTLFFEPSTRTRHSFHLAAQRLGADVLN
FDASMSSTSKGESACDTLKNLEAMGVRGFIVRHPEEIVIAQLAAVVGEGTVLINAGAGRSTHPTQGLLDMLTLCQAKGND
FSKLKLLFVGDIKHSRVARSNLHALRTLGAGQIRVCGPTALLPHDGLLSGCVVSQDFDAMLEGVDVLMMLRLQRERMEEG
LVPSLEHYHANYGLTAARLARAAPDAVVLHPGPINRGVEITDEVADGPQSWILRQASNGVMVRMAVLETLLG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=33.8658146964856, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=31.7307692307692, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1790703, Length=335, Percent_Identity=26.865671641791, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=294, Percent_Identity=34.0136054421769, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322331, Length=319, Percent_Identity=32.2884012539185, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=31.9488817891374, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33586; Mature: 33586

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDSNGRLRHLLTLEGLSRHTLLQLLDCAAQNCALGVDPMGKRNVLAGMAVCTLFFEPS
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEECCC
TRTRHSFHLAAQRLGADVLNFDASMSSTSKGESACDTLKNLEAMGVRGFIVRHPEEIVIA
CCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHH
QLAAVVGEGTVLINAGAGRSTHPTQGLLDMLTLCQAKGNDFSKLKLLFVGDIKHSRVARS
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHH
NLHALRTLGAGQIRVCGPTALLPHDGLLSGCVVSQDFDAMLEGVDVLMMLRLQRERMEEG
HHHHHHHCCCCEEEEECCCEECCCCCCHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHCC
LVPSLEHYHANYGLTAARLARAAPDAVVLHPGPINRGVEITDEVADGPQSWILRQASNGV
CCCCHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCCCEECHHHCCCHHHHHHHHCCCCE
MVRMAVLETLLG
EEHHHHHHHHCC
>Mature Secondary Structure
MQFDSNGRLRHLLTLEGLSRHTLLQLLDCAAQNCALGVDPMGKRNVLAGMAVCTLFFEPS
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEECCC
TRTRHSFHLAAQRLGADVLNFDASMSSTSKGESACDTLKNLEAMGVRGFIVRHPEEIVIA
CCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHH
QLAAVVGEGTVLINAGAGRSTHPTQGLLDMLTLCQAKGNDFSKLKLLFVGDIKHSRVARS
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHH
NLHALRTLGAGQIRVCGPTALLPHDGLLSGCVVSQDFDAMLEGVDVLMMLRLQRERMEEG
HHHHHHHCCCCEEEEECCCEECCCCCCHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHCC
LVPSLEHYHANYGLTAARLARAAPDAVVLHPGPINRGVEITDEVADGPQSWILRQASNGV
CCCCHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCCCEECHHHCCCHHHHHHHHCCCCE
MVRMAVLETLLG
EEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]