Click here to switch to the map view.

The map label for this gene is pilJ [H]

Identifier: 182681444

GI number: 182681444

Start: 1058481

End: 1060523

Strand: Direct

Name: pilJ [H]

Synonym: XfasM23_0895

Alternate gene names: 182681444

Gene position: 1058481-1060523 (Clockwise)

Preceding gene: 182681443

Following gene: 182681445

Centisome position: 41.74

GC content: 49.53

Gene sequence:

>2043_bases
ATGGGTAGCTCCCCGAATGTCCGCAACAGCCAAAAGTTTGACGGCCCGAGTACCAACTTTTGGATTGGTTTGTTCTTATT
GTCGATGATTGTATTTAGCGCCAATATCGGTGTGGCAACTTGGCAAGGCAGGCGGCTTGCTGGGGCCAGTGCCGATGTGG
CTAACTTGCAGGTGTTGTCGCAGCAGTTGGTTAGTCAGGGGCGCGAAGCGGTCGCTGGAGATTCAAAAGCGTTTTCCAAT
TTTAAGGAAATCAAAAGCAAGATTGAAGATACTGTCAAGAAGCTTGAGGCCCGCTATAACAATGAAAGTTCGGTTTCTGA
TGCTTTGTTCCAACTGAAAAATACTTGGGAGCCCTTGAGTAAGAATGCAAAGCAGTTAATTGATAGCGAATCGGCAACAT
TGGTTTTAGCGAGTAACGCTGATCGCTTCGTGAGTAATGTGCCTCAGCTTCAGGCACAATTAAACGAGGTGGTACGTGCG
ATGACTGCTAGTGGCGCGCCACCCTCGCAGTTCTACAATACACTGCAGCAGGTAGTAGTGGCCGGCACTATGGCGCGGCG
TGTCGCTGAGATGCGTGCTTCCGGGGTTGGGGCAGCCGCAGCCGGCGATGCGCTGGCGCGGGATTCTGTGATGTTCACAA
GGATGCTGGAAGGTTTGCGCAATGGCAATCAGGAGTTAGGGATATCCCCTGTTCGCAATGATGCCACGCTGTCAGCACTG
ACAAAGTCGCAGTCCCTGTGGATAACAATGAAAAAGGACGTTGATAATATCCTGGCGACTTCGCATCAACTATTTGCTGC
GCAATCTTCTGTCGATGCATTGGTGAATGGCTCAGGTAAATTATTGGAGGACAGTAATAAATTATTTAATGCGTTTTCGA
CATTTGGGGTTGGCTCGGTACGTGATAAACGTATTTTCCCCAATTTCTGGCTCACTGTGATATTTGGGTTGCTGTCTGTA
GTGTCGATTGTTGGTCTGGCCTGGAGTACGGTGATTTCCCGATCACGTGAACAAGAGCGGCGTTATCAAGCTCAGGTGGA
GTTTAATAGCCGTAACCAGCAGGCAATCATGCGATTGCTGGAAGAGATTATTTCCATAGGTGAAGGTGATCTAACCGTGA
AGGCTTCGGTCACCGAGGAAATGACTGGGGCGATAGCCGACGCGATTAACTGTGCTGTTGATGAACTGCGTCAATTGGTC
GCCACGATCAATACTACTTCGGCTAAGGTTGCGGTATCGGCGCATGAAACTCAAACCACTGCGATGCGCTTGGCGGAGGC
CGCAGGCCATCAAGCCAATCAAATCACCACAGTCTCAGAGCGAATTAGCGAGATTGCGGCTAGTATCGAACAGGTGTCAC
GCAACTCCGCAGAATCGGCAGAAGTAGCGCAGCGTTCTGTTGTGATTGCAGCAGAGGGTGCTGGTGTAGTCCGTGAAACT
ATCCAGGGCATGGACCAAATCCGCGATCAGATCCAGGAAACCTCAAAACGTATCAAGCGTCTGGGTGAGTCGTCTCAAGA
GATTGGTTCAATCGTAGAATTAATTAACGATATTTCTGAACAGACCAATATTTTGGCGCTTAATGCAGCAGTGCAAGCAG
CTTCTGCTGGCGAAGCAGGACGTGGTTTCGCCATTGTTGCCGATGAAGTCCAGCGTTTAGCTGAACGCACCTCTAGTGCA
ACCCGTCGCATCGAGAGTTTGGTACGGGCAATTCAGGTTGACACTAACGAGGCTGTTAGCTCAATGGAGCAGACGACTGC
TGAAGTGGTATCTGGCGCGCGCTTGGCTGAAGATGCAGGTACTGCATTGATTGAGATTGAACACGTATCTAACACGCTTA
ACCATTTAATTAAAAACATTTCCATTGCTGCTCATCAACAAGCAGCTGCTGCTACTGACATCACTCAAACGATGGGTGTA
ATTCGCCAAATTACTTCTCAGACCTCACAAGGTGCTGGGAAGACTGCTGAATCGATCGGTCGTTTAGCTGGGTTGGCTGC
TGAATTGCGTCGATCAGCAGCCAACTTCAAATTGCCAGCTTGA

Upstream 100 bases:

>100_bases
GTGCGTGTTCTCGCTTTCGCTGCTATCGCGGACACCTGAATTTAGACAGGCTGCTGCCTGATTGGCGGTCCCGTCTTGCA
TATTTAGATTGAGGTCGAAC

Downstream 100 bases:

>100_bases
ATGCTTCTCGGCTTGGAAAGGATGAGCAGATTGAAGTACAGCATCATATCAAAGCGAAATATGTGGTCACCTCATTCTTT
GAACGGTGGCGTGGCAGGGG

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MGSSPNVRNSQKFDGPSTNFWIGLFLLSMIVFSANIGVATWQGRRLAGASADVANLQVLSQQLVSQGREAVAGDSKAFSN
FKEIKSKIEDTVKKLEARYNNESSVSDALFQLKNTWEPLSKNAKQLIDSESATLVLASNADRFVSNVPQLQAQLNEVVRA
MTASGAPPSQFYNTLQQVVVAGTMARRVAEMRASGVGAAAAGDALARDSVMFTRMLEGLRNGNQELGISPVRNDATLSAL
TKSQSLWITMKKDVDNILATSHQLFAAQSSVDALVNGSGKLLEDSNKLFNAFSTFGVGSVRDKRIFPNFWLTVIFGLLSV
VSIVGLAWSTVISRSREQERRYQAQVEFNSRNQQAIMRLLEEIISIGEGDLTVKASVTEEMTGAIADAINCAVDELRQLV
ATINTTSAKVAVSAHETQTTAMRLAEAAGHQANQITTVSERISEIAASIEQVSRNSAESAEVAQRSVVIAAEGAGVVRET
IQGMDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAGRGFAIVADEVQRLAERTSSA
TRRIESLVRAIQVDTNEAVSSMEQTTAEVVSGARLAEDAGTALIEIEHVSNTLNHLIKNISIAAHQQAAAATDITQTMGV
IRQITSQTSQGAGKTAESIGRLAGLAAELRRSAANFKLPA

Sequences:

>Translated_680_residues
MGSSPNVRNSQKFDGPSTNFWIGLFLLSMIVFSANIGVATWQGRRLAGASADVANLQVLSQQLVSQGREAVAGDSKAFSN
FKEIKSKIEDTVKKLEARYNNESSVSDALFQLKNTWEPLSKNAKQLIDSESATLVLASNADRFVSNVPQLQAQLNEVVRA
MTASGAPPSQFYNTLQQVVVAGTMARRVAEMRASGVGAAAAGDALARDSVMFTRMLEGLRNGNQELGISPVRNDATLSAL
TKSQSLWITMKKDVDNILATSHQLFAAQSSVDALVNGSGKLLEDSNKLFNAFSTFGVGSVRDKRIFPNFWLTVIFGLLSV
VSIVGLAWSTVISRSREQERRYQAQVEFNSRNQQAIMRLLEEIISIGEGDLTVKASVTEEMTGAIADAINCAVDELRQLV
ATINTTSAKVAVSAHETQTTAMRLAEAAGHQANQITTVSERISEIAASIEQVSRNSAESAEVAQRSVVIAAEGAGVVRET
IQGMDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAGRGFAIVADEVQRLAERTSSA
TRRIESLVRAIQVDTNEAVSSMEQTTAEVVSGARLAEDAGTALIEIEHVSNTLNHLIKNISIAAHQQAAAATDITQTMGV
IRQITSQTSQGAGKTAESIGRLAGLAAELRRSAANFKLPA
>Mature_679_residues
GSSPNVRNSQKFDGPSTNFWIGLFLLSMIVFSANIGVATWQGRRLAGASADVANLQVLSQQLVSQGREAVAGDSKAFSNF
KEIKSKIEDTVKKLEARYNNESSVSDALFQLKNTWEPLSKNAKQLIDSESATLVLASNADRFVSNVPQLQAQLNEVVRAM
TASGAPPSQFYNTLQQVVVAGTMARRVAEMRASGVGAAAAGDALARDSVMFTRMLEGLRNGNQELGISPVRNDATLSALT
KSQSLWITMKKDVDNILATSHQLFAAQSSVDALVNGSGKLLEDSNKLFNAFSTFGVGSVRDKRIFPNFWLTVIFGLLSVV
SIVGLAWSTVISRSREQERRYQAQVEFNSRNQQAIMRLLEEIISIGEGDLTVKASVTEEMTGAIADAINCAVDELRQLVA
TINTTSAKVAVSAHETQTTAMRLAEAAGHQANQITTVSERISEIAASIEQVSRNSAESAEVAQRSVVIAAEGAGVVRETI
QGMDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAGRGFAIVADEVQRLAERTSSAT
RRIESLVRAIQVDTNEAVSSMEQTTAEVVSGARLAEDAGTALIEIEHVSNTLNHLIKNISIAAHQQAAAATDITQTMGVI
RQITSQTSQGAGKTAESIGRLAGLAAELRRSAANFKLPA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=292, Percent_Identity=29.4520547945205, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788194, Length=249, Percent_Identity=30.1204819277108, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1789453, Length=251, Percent_Identity=30.6772908366534, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787690, Length=217, Percent_Identity=30.8755760368664, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788195, Length=309, Percent_Identity=26.8608414239482, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 72793; Mature: 72662

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSPNVRNSQKFDGPSTNFWIGLFLLSMIVFSANIGVATWQGRRLAGASADVANLQVLS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEECCCCCHHHHHHHHH
QQLVSQGREAVAGDSKAFSNFKEIKSKIEDTVKKLEARYNNESSVSDALFQLKNTWEPLS
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KNAKQLIDSESATLVLASNADRFVSNVPQLQAQLNEVVRAMTASGAPPSQFYNTLQQVVV
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AGTMARRVAEMRASGVGAAAAGDALARDSVMFTRMLEGLRNGNQELGISPVRNDATLSAL
HHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
TKSQSLWITMKKDVDNILATSHQLFAAQSSVDALVNGSGKLLEDSNKLFNAFSTFGVGSV
HCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCC
RDKRIFPNFWLTVIFGLLSVVSIVGLAWSTVISRSREQERRYQAQVEFNSRNQQAIMRLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHH
EEIISIGEGDLTVKASVTEEMTGAIADAINCAVDELRQLVATINTTSAKVAVSAHETQTT
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHH
AMRLAEAAGHQANQITTVSERISEIAASIEQVSRNSAESAEVAQRSVVIAAEGAGVVRET
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCHHHHHH
IQGMDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCC
RGFAIVADEVQRLAERTSSATRRIESLVRAIQVDTNEAVSSMEQTTAEVVSGARLAEDAG
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
TALIEIEHVSNTLNHLIKNISIAAHQQAAAATDITQTMGVIRQITSQTSQGAGKTAESIG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RLAGLAAELRRSAANFKLPA
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GSSPNVRNSQKFDGPSTNFWIGLFLLSMIVFSANIGVATWQGRRLAGASADVANLQVLS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEECCCCCHHHHHHHHH
QQLVSQGREAVAGDSKAFSNFKEIKSKIEDTVKKLEARYNNESSVSDALFQLKNTWEPLS
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KNAKQLIDSESATLVLASNADRFVSNVPQLQAQLNEVVRAMTASGAPPSQFYNTLQQVVV
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AGTMARRVAEMRASGVGAAAAGDALARDSVMFTRMLEGLRNGNQELGISPVRNDATLSAL
HHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
TKSQSLWITMKKDVDNILATSHQLFAAQSSVDALVNGSGKLLEDSNKLFNAFSTFGVGSV
HCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCC
RDKRIFPNFWLTVIFGLLSVVSIVGLAWSTVISRSREQERRYQAQVEFNSRNQQAIMRLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHH
EEIISIGEGDLTVKASVTEEMTGAIADAINCAVDELRQLVATINTTSAKVAVSAHETQTT
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHH
AMRLAEAAGHQANQITTVSERISEIAASIEQVSRNSAESAEVAQRSVVIAAEGAGVVRET
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCHHHHHH
IQGMDQIRDQIQETSKRIKRLGESSQEIGSIVELINDISEQTNILALNAAVQAASAGEAG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCC
RGFAIVADEVQRLAERTSSATRRIESLVRAIQVDTNEAVSSMEQTTAEVVSGARLAEDAG
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
TALIEIEHVSNTLNHLIKNISIAAHQQAAAATDITQTMGVIRQITSQTSQGAGKTAESIG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RLAGLAAELRRSAANFKLPA
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]