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The map label for this gene is glyS

Identifier: 182681438

GI number: 182681438

Start: 1051806

End: 1053974

Strand: Direct

Name: glyS

Synonym: XfasM23_0889

Alternate gene names: 182681438

Gene position: 1051806-1053974 (Clockwise)

Preceding gene: 182681437

Following gene: 182681442

Centisome position: 41.48

GC content: 50.62

Gene sequence:

>2169_bases
ATGACTGAACTAAAACCACTGCTGATCGAACTTGGGACCGAGGAATTGCCGGTCAATGCTTTGCCGAACTTGTCGCGAGC
ATTTTTCGAGGGGGTGCTGGCTGGGTTGGAGAGGCGCGGCATTGTGGTCGATCATGGTGAGGCCAAGTCTCTGTCGACTC
CGCGTCGGCTTGCTGTGTTACTCACGGCTGTGGCAGTGGAGCAGCCAAAGCAGTATCGCGAATTGTTTGGCCCTTATCTG
AATACTGCGTTTGATACCGAAGGTAAGCCCACGAAGGCGTTGGAGGGGTTTGCTGCTAAATGTGGTGTCAATTGGAGGAC
ATTGGAGCGTATTCGTGATACCAAGGGCGAGCGTTTCGTACATCGGGTGGTGGTGCCTGGCGAGCGTACGGATGCGTTGT
TGCCAGGAATATTGACTGAAGCAATTGCGGGGATGCCTATTCCCAAGCCGATGCGCTGGGGCGCTCATGAATATCTATTT
GCACGTCCAGTCCATTGGTTGGTGATGCTTTTTGGGCAGGATGTGGTTGAGGCTGAGATTATGGGGGTGAAGGCTGGTCG
TATCAGTCGTGGTCATCGCTTTTTGTATGACAAGCCGGTGTCGCTGAGTGGTCCGCAGAATTATATTGATGTTCTTCAGG
CTGCTTATGTGTTGGTGGATCCTGCCGCACGTCGTGCTCGCATTGTTGCTGAGATTGAGGCCGTAGCGCGTCAGGTTGGT
GGAGTCGCGCGGATCACTGAAGATAATGTGGCGCAGGTGGTGAATTTGGTGGAATGGCCGTCGGCGGTGCTATGTAGCTT
TGAGCGTGTGTTTCTTGAGGTGCCGCAGGAGGCGTTGATCCAAACAATGGAGGTGAATCAGAAATTTTTTCCTGTACTCG
ATAGTTTGGGGAAGTTGACTGAGAAGTTCATCGGTATTGCCAATATTGAGTCCAATGATGTTGCTGAGGTCGCAAAGGGC
TATGAACGTGTGATCCGTCCGCGTTTTTCTGATGCTAAGTTTTTCTTCAATGAGGATCTCAAGCAAGGTTTGAAAGCAAT
GGGAGAGCGACTAAGGACGGTCACCTATCATGCGAAGTTAGGTACGCTGGCTGACAAGGTTGCGCGAGTTCTGGTACTGG
CTGAAGCGATTGCGCCACAGATTGGGGTAGATCCATTGCTTGCCAGGCGTGTTGCGTTGCTCAGTAAAAACGACTTGCAA
TCTCGCATGGTCAATGAGTTTCCGGAATTGCAGGGAGTTGCGGGTCATCACTATGCCTTGATTAGTGGTGAGTTACCCGA
GGTCGCAATGGCGATTGAGGATGCTTATCGGCCTCGCTTTTCTGGTGATGAGATCGCACGTTCGCCGTTGGGTAAGGTGC
TCGGACTCGCCGAGCGTCTGGATACGCTGGCGTGTGGTTTTGCTGTGGGAATGAAGCCAACTGGTAACAAGGATCCTTTT
GGGTTACGCCGCAATGCGTTGGGATTGGCAAGAACAATTATTGAGAGTCGGTTCGATCTGGATTTAAAAGTGCTACTGGA
TCAAGCGTCAGACTGGGTCGCGTTTGCGACGACGATTGAGCAGGAGCGTCATGCACAGGAATCAGTGAAACAGTCGAAAA
AAGAAGCGGCTGTTAAGCATTCGGTGCCCCAGGTCAGTGATGAGAAATCTGCAAGAATTGAAGAGCTTTACGATTTCATC
GTAGAGCGTTTGCGTGGTTACTATGCGGATAAGGGCATTCCAACAACGCACTTTAATGCAGTCGCTGAGCTTAAACCTGT
CTCTTTATACGATTTTCATCGCCGTCTTGATGCAATCGGCAGATTTGCTGCTCTTCCAGAGGCAGAGGCGCTGGCAGTTG
CCAACAAGCGTATCCGTAATATTTTGCGTAAGGCTGAAATTAAGATTCCAGCATCAGTCGATGCGACATTGTTCGATCAA
CCAGCAGAGAGTGGGTTACTCGTGGCGTTGGAAGGTGTGATCACCGATACCCGCAGTGCATTAGATTGCAAAAATTATGT
CAGTGTCCTTACATGTCTGGCACGGTTACGGCCACCCATTGATGAGTTCTTCGACAAGGTGATGGTTAACGATGAAAATC
TGATGCTGCGTGCTAACCGCTTAGCGTTACTGCAACGCTTAGGGGAGTATCTGTGTTGTGTTGCGGCCATTGAACACTTG
TCTAATTGA

Upstream 100 bases:

>100_bases
AGGCGCTAGTTGCCTCCTTTATCCGTTTTCCGTTTCGAATCACTGTGGTGCGCCGCTAGTTGCCATTCGCGGAAGAAACG
GAGACCAAGGTCCTATTGTC

Downstream 100 bases:

>100_bases
TTGAGAGTTGCAGTTTTTTATGGGGTCAAAAGTATGTATAGCTTGGGTCTCAAGATGAGGTCTATAAGCACTGTGATTCA
GTATTTCTAAGTGGATTGAT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MTELKPLLIELGTEELPVNALPNLSRAFFEGVLAGLERRGIVVDHGEAKSLSTPRRLAVLLTAVAVEQPKQYRELFGPYL
NTAFDTEGKPTKALEGFAAKCGVNWRTLERIRDTKGERFVHRVVVPGERTDALLPGILTEAIAGMPIPKPMRWGAHEYLF
ARPVHWLVMLFGQDVVEAEIMGVKAGRISRGHRFLYDKPVSLSGPQNYIDVLQAAYVLVDPAARRARIVAEIEAVARQVG
GVARITEDNVAQVVNLVEWPSAVLCSFERVFLEVPQEALIQTMEVNQKFFPVLDSLGKLTEKFIGIANIESNDVAEVAKG
YERVIRPRFSDAKFFFNEDLKQGLKAMGERLRTVTYHAKLGTLADKVARVLVLAEAIAPQIGVDPLLARRVALLSKNDLQ
SRMVNEFPELQGVAGHHYALISGELPEVAMAIEDAYRPRFSGDEIARSPLGKVLGLAERLDTLACGFAVGMKPTGNKDPF
GLRRNALGLARTIIESRFDLDLKVLLDQASDWVAFATTIEQERHAQESVKQSKKEAAVKHSVPQVSDEKSARIEELYDFI
VERLRGYYADKGIPTTHFNAVAELKPVSLYDFHRRLDAIGRFAALPEAEALAVANKRIRNILRKAEIKIPASVDATLFDQ
PAESGLLVALEGVITDTRSALDCKNYVSVLTCLARLRPPIDEFFDKVMVNDENLMLRANRLALLQRLGEYLCCVAAIEHL
SN

Sequences:

>Translated_722_residues
MTELKPLLIELGTEELPVNALPNLSRAFFEGVLAGLERRGIVVDHGEAKSLSTPRRLAVLLTAVAVEQPKQYRELFGPYL
NTAFDTEGKPTKALEGFAAKCGVNWRTLERIRDTKGERFVHRVVVPGERTDALLPGILTEAIAGMPIPKPMRWGAHEYLF
ARPVHWLVMLFGQDVVEAEIMGVKAGRISRGHRFLYDKPVSLSGPQNYIDVLQAAYVLVDPAARRARIVAEIEAVARQVG
GVARITEDNVAQVVNLVEWPSAVLCSFERVFLEVPQEALIQTMEVNQKFFPVLDSLGKLTEKFIGIANIESNDVAEVAKG
YERVIRPRFSDAKFFFNEDLKQGLKAMGERLRTVTYHAKLGTLADKVARVLVLAEAIAPQIGVDPLLARRVALLSKNDLQ
SRMVNEFPELQGVAGHHYALISGELPEVAMAIEDAYRPRFSGDEIARSPLGKVLGLAERLDTLACGFAVGMKPTGNKDPF
GLRRNALGLARTIIESRFDLDLKVLLDQASDWVAFATTIEQERHAQESVKQSKKEAAVKHSVPQVSDEKSARIEELYDFI
VERLRGYYADKGIPTTHFNAVAELKPVSLYDFHRRLDAIGRFAALPEAEALAVANKRIRNILRKAEIKIPASVDATLFDQ
PAESGLLVALEGVITDTRSALDCKNYVSVLTCLARLRPPIDEFFDKVMVNDENLMLRANRLALLQRLGEYLCCVAAIEHL
SN
>Mature_721_residues
TELKPLLIELGTEELPVNALPNLSRAFFEGVLAGLERRGIVVDHGEAKSLSTPRRLAVLLTAVAVEQPKQYRELFGPYLN
TAFDTEGKPTKALEGFAAKCGVNWRTLERIRDTKGERFVHRVVVPGERTDALLPGILTEAIAGMPIPKPMRWGAHEYLFA
RPVHWLVMLFGQDVVEAEIMGVKAGRISRGHRFLYDKPVSLSGPQNYIDVLQAAYVLVDPAARRARIVAEIEAVARQVGG
VARITEDNVAQVVNLVEWPSAVLCSFERVFLEVPQEALIQTMEVNQKFFPVLDSLGKLTEKFIGIANIESNDVAEVAKGY
ERVIRPRFSDAKFFFNEDLKQGLKAMGERLRTVTYHAKLGTLADKVARVLVLAEAIAPQIGVDPLLARRVALLSKNDLQS
RMVNEFPELQGVAGHHYALISGELPEVAMAIEDAYRPRFSGDEIARSPLGKVLGLAERLDTLACGFAVGMKPTGNKDPFG
LRRNALGLARTIIESRFDLDLKVLLDQASDWVAFATTIEQERHAQESVKQSKKEAAVKHSVPQVSDEKSARIEELYDFIV
ERLRGYYADKGIPTTHFNAVAELKPVSLYDFHRRLDAIGRFAALPEAEALAVANKRIRNILRKAEIKIPASVDATLFDQP
AESGLLVALEGVITDTRSALDCKNYVSVLTCLARLRPPIDEFFDKVMVNDENLMLRANRLALLQRLGEYLCCVAAIEHLS
N

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=729, Percent_Identity=40.0548696844993, Blast_Score=502, Evalue=1e-143,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_XYLF2 (B2I4M9)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829598.1
- ProteinModelPortal:   B2I4M9
- GeneID:   6203451
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0889
- HOGENOM:   HBG586599
- OMA:   SNITYGH
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 80123; Mature: 79991

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELKPLLIELGTEELPVNALPNLSRAFFEGVLAGLERRGIVVDHGEAKSLSTPRRLAVL
CCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHH
LTAVAVEQPKQYRELFGPYLNTAFDTEGKPTKALEGFAAKCGVNWRTLERIRDTKGERFV
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
HRVVVPGERTDALLPGILTEAIAGMPIPKPMRWGAHEYLFARPVHWLVMLFGQDVVEAEI
HHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
MGVKAGRISRGHRFLYDKPVSLSGPQNYIDVLQAAYVLVDPAARRARIVAEIEAVARQVG
HCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GVARITEDNVAQVVNLVEWPSAVLCSFERVFLEVPQEALIQTMEVNQKFFPVLDSLGKLT
CEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHH
EKFIGIANIESNDVAEVAKGYERVIRPRFSDAKFFFNEDLKQGLKAMGERLRTVTYHAKL
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTLADKVARVLVLAEAIAPQIGVDPLLARRVALLSKNDLQSRMVNEFPELQGVAGHHYAL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEE
ISGELPEVAMAIEDAYRPRFSGDEIARSPLGKVLGLAERLDTLACGFAVGMKPTGNKDPF
ECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GLRRNALGLARTIIESRFDLDLKVLLDQASDWVAFATTIEQERHAQESVKQSKKEAAVKH
CHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVPQVSDEKSARIEELYDFIVERLRGYYADKGIPTTHFNAVAELKPVSLYDFHRRLDAIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
RFAALPEAEALAVANKRIRNILRKAEIKIPASVDATLFDQPAESGLLVALEGVITDTRSA
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEHHHHHHHHH
LDCKNYVSVLTCLARLRPPIDEFFDKVMVNDENLMLRANRLALLQRLGEYLCCVAAIEHL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
SN
CC
>Mature Secondary Structure 
TELKPLLIELGTEELPVNALPNLSRAFFEGVLAGLERRGIVVDHGEAKSLSTPRRLAVL
CCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHH
LTAVAVEQPKQYRELFGPYLNTAFDTEGKPTKALEGFAAKCGVNWRTLERIRDTKGERFV
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
HRVVVPGERTDALLPGILTEAIAGMPIPKPMRWGAHEYLFARPVHWLVMLFGQDVVEAEI
HHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
MGVKAGRISRGHRFLYDKPVSLSGPQNYIDVLQAAYVLVDPAARRARIVAEIEAVARQVG
HCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GVARITEDNVAQVVNLVEWPSAVLCSFERVFLEVPQEALIQTMEVNQKFFPVLDSLGKLT
CEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHH
EKFIGIANIESNDVAEVAKGYERVIRPRFSDAKFFFNEDLKQGLKAMGERLRTVTYHAKL
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTLADKVARVLVLAEAIAPQIGVDPLLARRVALLSKNDLQSRMVNEFPELQGVAGHHYAL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEE
ISGELPEVAMAIEDAYRPRFSGDEIARSPLGKVLGLAERLDTLACGFAVGMKPTGNKDPF
ECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GLRRNALGLARTIIESRFDLDLKVLLDQASDWVAFATTIEQERHAQESVKQSKKEAAVKH
CHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVPQVSDEKSARIEELYDFIVERLRGYYADKGIPTTHFNAVAELKPVSLYDFHRRLDAIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
RFAALPEAEALAVANKRIRNILRKAEIKIPASVDATLFDQPAESGLLVALEGVITDTRSA
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEHHHHHHHHH
LDCKNYVSVLTCLARLRPPIDEFFDKVMVNDENLMLRANRLALLQRLGEYLCCVAAIEHL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
SN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA