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The map label for this gene is cysD [H]

Identifier: 182681306

GI number: 182681306

Start: 889690

End: 890673

Strand: Direct

Name: cysD [H]

Synonym: XfasM23_0753

Alternate gene names: 182681306

Gene position: 889690-890673 (Clockwise)

Preceding gene: 182681301

Following gene: 182681307

Centisome position: 35.09

GC content: 52.44

Gene sequence:

>984_bases
ATGTTTTCGGTTGGCTACGCTGTCCGCCGATGTGTACATCACTCCAATTCTCTAGAGTTGCTACCTCTGATGGATTCATC
CTCTTTCTCTTTTTCTCATCTTGATCGGCTTGAGGCTGAAAGTATCTACATCTTGCGTGAGGTCGTGGCGGAATTCCGTA
ATCCGGTGTTGCTTTACTCTGTGGGTAAGGATAGTTCAGTACTGTTGCACCTGTTACTTAAGGCGTTTGCGCCGGCACCG
CCACCGATTCCGCTACTGCATGTCGATACGCGTTGGAAGTTTCGCGAGATGATTGCCTTTCGTGATCGCCGTGTTGCTGA
GACGGGGGTACAGTTGCGTGTCCACATTAATCTTGAGGGTGTGGCGCAGGAGATTAATCCGATCACTCACGGTGCCGCTG
TGCATACGGACATTATGAAAACGCAGGGGTTGCGGCAGGCGTTGGAGCAGGGGCAGTTTGATGCGGCGATTGGTGGAGCA
CGCCGCGATGAAGAAAAGTCGCGTGCCAAGGAGCGTGTATTCTCGTTTCGTAACGCCCATCATCGATGGGATCCCAAAAA
CCAACGTCCTGAATTGTGGAATGTGTACAACGCGCGTATTCATCCTGGAGAGAGCGTGCGGGTATTTCCACTGTCTAATT
GGACTGAATTGGATGTGTGGTTGTACATCTATCGTGAGCAGATTCCGGTGGTGTCGTTATATTTTGCTGCACCGCGCCCA
GTGGTTGAGCGCGACGGTATGTTGATTCTGGTTGACGATGAGCGTTTGCCCCTGCATCCGGGCGAAGTGTCGAAGTTGCG
TTGGGTGCGCTTCCGTACCTTGGGTTGCTATCCCCTGACTGGGGCGGTTGAGTCTCGTGCTGCGACTTTAGAAGACATCA
TTGCTGAGATGCTGGTGACTCCCTTTTCGGAGCGTCAGGGGCGGCTGATCGATTATGCCCCTGGTGCCTCAATGGAAAGC
AAAAAGATAGAGGGGTATTTCTGA

Upstream 100 bases:

>100_bases
GATGTTAGGGAGGTGCGAAATGGTGCGGAAAGGAAGGAAGGTTATCCCCTTATGCTTCGTGCTTATTTCAGAACATGGTT
AATTTCGCTACACACTGCGC

Downstream 100 bases:

>100_bases
TGATTGCTCGGATGGTGAACGTGAAACGCGCGTTTGACTGGTCAGAGGCAAGCACAAGTGTGGATGCGTTGCCGGTTCCT
GAGACTGTCACCTATAGCGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MFSVGYAVRRCVHHSNSLELLPLMDSSSFSFSHLDRLEAESIYILREVVAEFRNPVLLYSVGKDSSVLLHLLLKAFAPAP
PPIPLLHVDTRWKFREMIAFRDRRVAETGVQLRVHINLEGVAQEINPITHGAAVHTDIMKTQGLRQALEQGQFDAAIGGA
RRDEEKSRAKERVFSFRNAHHRWDPKNQRPELWNVYNARIHPGESVRVFPLSNWTELDVWLYIYREQIPVVSLYFAAPRP
VVERDGMLILVDDERLPLHPGEVSKLRWVRFRTLGCYPLTGAVESRAATLEDIIAEMLVTPFSERQGRLIDYAPGASMES
KKIEGYF

Sequences:

>Translated_327_residues
MFSVGYAVRRCVHHSNSLELLPLMDSSSFSFSHLDRLEAESIYILREVVAEFRNPVLLYSVGKDSSVLLHLLLKAFAPAP
PPIPLLHVDTRWKFREMIAFRDRRVAETGVQLRVHINLEGVAQEINPITHGAAVHTDIMKTQGLRQALEQGQFDAAIGGA
RRDEEKSRAKERVFSFRNAHHRWDPKNQRPELWNVYNARIHPGESVRVFPLSNWTELDVWLYIYREQIPVVSLYFAAPRP
VVERDGMLILVDDERLPLHPGEVSKLRWVRFRTLGCYPLTGAVESRAATLEDIIAEMLVTPFSERQGRLIDYAPGASMES
KKIEGYF
>Mature_327_residues
MFSVGYAVRRCVHHSNSLELLPLMDSSSFSFSHLDRLEAESIYILREVVAEFRNPVLLYSVGKDSSVLLHLLLKAFAPAP
PPIPLLHVDTRWKFREMIAFRDRRVAETGVQLRVHINLEGVAQEINPITHGAAVHTDIMKTQGLRQALEQGQFDAAIGGA
RRDEEKSRAKERVFSFRNAHHRWDPKNQRPELWNVYNARIHPGESVRVFPLSNWTELDVWLYIYREQIPVVSLYFAAPRP
VVERDGMLILVDDERLPLHPGEVSKLRWVRFRTLGCYPLTGAVESRAATLEDIIAEMLVTPFSERQGRLIDYAPGASMES
KKIEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=67.6767676767677, Blast_Score=416, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 37480; Mature: 37480

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSVGYAVRRCVHHSNSLELLPLMDSSSFSFSHLDRLEAESIYILREVVAEFRNPVLLYS
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VGKDSSVLLHLLLKAFAPAPPPIPLLHVDTRWKFREMIAFRDRRVAETGVQLRVHINLEG
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEHHH
VAQEINPITHGAAVHTDIMKTQGLRQALEQGQFDAAIGGARRDEEKSRAKERVFSFRNAH
HHHHCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCC
HRWDPKNQRPELWNVYNARIHPGESVRVFPLSNWTELDVWLYIYREQIPVVSLYFAAPRP
CCCCCCCCCCCCEEEEECEECCCCCEEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCC
VVERDGMLILVDDERLPLHPGEVSKLRWVRFRTLGCYPLTGAVESRAATLEDIIAEMLVT
CCCCCCEEEEECCCCCCCCCCCHHHEEEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHC
PFSERQGRLIDYAPGASMESKKIEGYF
CCCCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MFSVGYAVRRCVHHSNSLELLPLMDSSSFSFSHLDRLEAESIYILREVVAEFRNPVLLYS
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VGKDSSVLLHLLLKAFAPAPPPIPLLHVDTRWKFREMIAFRDRRVAETGVQLRVHINLEG
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEHHH
VAQEINPITHGAAVHTDIMKTQGLRQALEQGQFDAAIGGARRDEEKSRAKERVFSFRNAH
HHHHCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCC
HRWDPKNQRPELWNVYNARIHPGESVRVFPLSNWTELDVWLYIYREQIPVVSLYFAAPRP
CCCCCCCCCCCCEEEEECEECCCCCEEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCC
VVERDGMLILVDDERLPLHPGEVSKLRWVRFRTLGCYPLTGAVESRAATLEDIIAEMLVT
CCCCCCEEEEECCCCCCCCCCCHHHEEEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHC
PFSERQGRLIDYAPGASMESKKIEGYF
CCCCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]